{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T09:14:13Z","timestamp":1698830053064},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,3,31]],"date-time":"2011-03-31T00:00:00Z","timestamp":1301529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Intragastric (i.g.) immunization with recombinant chimeric proteins constructed from the saliva\u2010binding region (SBR) of <jats:italic>Streptococcus mutans<\/jats:italic> surface antigen AgI\/II and the A2\/B subunits of enterobacterial heat\u2010labile enterotoxins has been successfully used to induce salivary and circulating antibodies against <jats:italic>S.\u00a0mutans<\/jats:italic> that have protective potential against dental caries. To investigate the mode of action of these vaccine constructs, mice were immunized i.g. with chimeric proteins constructed from SBR and cholera toxin (CT) or the type II enterotoxins of <jats:italic>Escherichia coli<\/jats:italic>, LT\u2010IIa and LT\u2010IIb. Antigen\u2010presenting cells (APC) in Peyer\u2019s patches (PP) and mesenteric lymph nodes (MLN) were characterized by flow cytometry. Compared with immunization with SBR alone, chimeric proteins SBR\u2010LTIIaA2\/B and SBR\u2010LTIIbA2\/B increased the number of B cells and macrophages in PP and diminished B cell numbers in MLN, whereas SBR\u2010CTA2\/B diminished the numbers of B cells and macrophages in PP and MLN. Immunization with all three chimeric proteins led to upregulation of MHC class II molecules and co\u2010stimulatory receptors CD40, CD80, and CD86 especially on dendritic cells in PP and also on APC in MLN. The results provide a molecular basis for the enhanced immune responses induced by chimeric proteins compared with uncoupled antigen, and for differential responses to chimeric proteins based on CT or type II enterotoxins.<\/jats:p>","DOI":"10.1111\/j.2041-1014.2011.00608.x","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T11:26:16Z","timestamp":1301657176000},"page":"200-209","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of antigen\u2010presenting cells induced by intragastric immunization with recombinant chimeric immunogens constructed from <i>Streptococcus mutans<\/i> AgI\/II and type I or type II heat\u2010labile 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