{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:01:29Z","timestamp":1760781689600},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,11,15]],"date-time":"2011-11-15T00:00:00Z","timestamp":1321315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Periodontal diseases result from the interaction of bacterial pathogens with the host\u2019s gingival tissue. Gingival epithelial cells are constantly challenged by microbial cells and respond by altering their transcription profiles, inducing the production of inflammatory mediators. Different transcription profiles are induced by oral bacteria and little is known about how the gingival epithelium responds after interaction with the periodontopathogenic organism <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic>. In the present study, we examined the transcription of genes involved in signaling transduction pathways in gingival epithelial cells exposed to viable <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic>. Immortalized gingival epithelial cells (OBA\u20109) were infected with <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic> JP2 for 24\u2003h and the transcription profile of genes encoding human signal transduction pathways was determined. Functional analysis of inflammatory mediators positively transcribed was performed by ELISA in culture supernatant and in gingival tissues. Fifteen of 84 genes on the array were over\u2010expressed (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01) after 24\u2003h of infection with viable <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic>. Over\u2010expressed genes included those implicated in tissue remodeling and bone resorption, such as CSF2, genes encoding components of the LDL pathway, nuclear factor\u2010\u03baB\u2010dependent genes and other cytokines. The ELISA data confirmed that granulocyte\u2013macrophage colony\u2010stimulating factor\/colony\u2010stimulating factor 2, tumor necrosis factor\u2010\u03b1 and intercellular adhesion molecule\u20101 were highly expressed by infected gingival cells when compared with control non\u2010infected cells, and presented higher concentrations in tissues from patients with aggressive and chronic periodontitis than in tissues from healthy controls. The induction in epithelial cells of factors such as the pro\u2010inflammatory cytokine CSF2, which is involved in osteoclastogenesis, may help to explain the outcomes of <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic> infection.<\/jats:p>","DOI":"10.1111\/j.2041-1014.2011.00629.x","type":"journal-article","created":{"date-parts":[[2011,11,16]],"date-time":"2011-11-16T07:46:34Z","timestamp":1321429594000},"page":"23-33","source":"Crossref","is-referenced-by-count":22,"title":["Signaling transduction analysis in gingival epithelial cells after infection with <i>Aggregatibacter 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