{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:39:16Z","timestamp":1762004356474},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,12,7]],"date-time":"2011-12-07T00:00:00Z","timestamp":1323216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We previously reported that lipopolysaccharide (LPS) \u2010related sugars are associated with the glycosylation of the collagen adhesin EmaA, a virulence determinant of <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic>. In this study, the role of LPS in the secretion of other virulence factors was investigated. The secretion of the epithelial adhesin Aae, the immunoglobulin Fc receptor Omp34 and leukotoxin were examined in a mutant strain with inactivated TDP\u20104\u2010keto\u20106\u2010deoxy\u2010<jats:sc>d\u2010<\/jats:sc>glucose 3,5\u2010epimerase (<jats:italic>rmlC<\/jats:italic>), which resulted in altered O\u2010antigen polysaccharides (O\u2010PS) of LPS. The secretion of Aae and Omp34 was not affected. However, the leukotoxin secretion, which is mediated by the TolC\u2010dependent type I secretion system, was altered in the <jats:italic>rmlC<\/jats:italic> mutant. The amount of secreted leukotoxin in the bacterial growth medium was reduced nine\u2010fold, with a concurrent four\u2010fold increase of the membrane\u2010bound toxin in the mutant compared with the wild\u2010type strain. The altered leukotoxin secretion pattern was restored to the wild\u2010type by complementation of the <jats:italic>rmlC<\/jats:italic> gene in <jats:italic>trans<\/jats:italic>. Examination of the <jats:italic>ltxA<\/jats:italic> mRNA levels indicated that the leukotoxin secretion was post\u2010transcriptionally regulated in the modified O\u2010PS containing strain. The mutant strain also showed increased resistance to vancomycin, an antibiotic dependent on TolC for internalization, indicating that TolC was affected. Overexpression of TolC in the <jats:italic>rmlC<\/jats:italic> mutant resulted in an increased TolC level in the outer membrane but did not restore the leukotoxin secretion profile to the wild\u2010type phenotype. The data suggest that O\u2010PS mediate leukotoxin secretion in <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.2041-1014.2011.00632.x","type":"journal-article","created":{"date-parts":[[2011,12,7]],"date-time":"2011-12-07T07:48:33Z","timestamp":1323244113000},"page":"70-82","source":"Crossref","is-referenced-by-count":17,"title":["Lipopolysaccharides mediate leukotoxin secretion in <i>Aggregatibacter actinomycetemcomitans<\/i>"],"prefix":"10.1111","volume":"27","author":[{"given":"G.","family":"Tang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Kawai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Komatsuzawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.P.","family":"Mintz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,12,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.03987.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.7.4681-4685.2001"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211177200"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02051-07"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2006.10.004"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00116-5"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1128\/mr.57.4.995-1017.1993","article-title":"ABC transporter: bacterial exporters","volume":"57","author":"Fath M.J.","year":"1993","journal-title":"Microbiol Rev"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1128\/jb.115.3.717-722.1973","article-title":"Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium\u2010laurayl sarcosinate","volume":"115","author":"Filip C.","year":"1973","journal-title":"J Bacteriol"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2011.00617.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.013383-0"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00548-08"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-200X.2009.02899.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60135-X"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.68.4.692-744.2004"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1998.tb01110.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"crossref","first-page":"4841","DOI":"10.1128\/JB.00326-06","article-title":"Polar localization of the autotransporter family of large bacterial virulence proteins","volume":"188","author":"Jain S.","year":"2006","journal-title":"J Bacteriol"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.2001.0474"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2006.02.004"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1128\/IAI.67.2.942-945.1999","article-title":"Cloning of the gene encoding the Actinobacillus actinomycetemcomitans serotype b OmpA\u2010like outer membrane protein","volume":"67","author":"Komatsuzawa H.","year":"1999","journal-title":"Infect Immun"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(02)00500-0"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01661.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/35016007"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.73.011303.074104"},{"key":"e_1_2_6_25_1","doi-asserted-by":"crossref","first-page":"15451","DOI":"10.1016\/S0021-9258(19)84850-0","article-title":"Analysis of the Actinobacillus actinomycetemcomitans leukotoxin gene. 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