{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T14:13:29Z","timestamp":1769350409255,"version":"3.49.0"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,11,12]],"date-time":"2012-11-12T00:00:00Z","timestamp":1352678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2013,5]]},"abstract":"Summary<\/jats:title>\n \n Reasons for performing study<\/jats:title>\n Equine joint infection is a life\u2010threatening disorder, and confirmation of the diagnosis can be difficult. Synovial fluid biomarkers may assist the discrimination between infectious and noninfectious joint disease.<\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n This study investigates whether the immunological detection of total and enzymatically active myeloperoxidase (\n MPO<\/jats:styled-content>\n ) assists the diagnosis of joint infection in horses.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The following 4 sample groups were included: healthy; osteochondritis dissecans (\n OCD<\/jats:styled-content>\n ); traumatic synovitis; and culture\u2010confirmed infected joints. Synovial fluid was analysed for total\n MPO<\/jats:styled-content>\n by a horse\u2010specific sandwich enzyme\u2010linked immunosorbent assay (\n ELISA<\/jats:styled-content>\n ) and for active\n MPO<\/jats:styled-content>\n using the specific immunological extraction followed by enzymatic detection (\n SIEFED<\/jats:styled-content>\n ) technique. Western blot analysis was performed to confirm the antibody specificity.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Synovial fluid from infected joints contained significantly more total and active\n MPO<\/jats:styled-content>\n than samples from healthy joints, joints affected by\n OCD<\/jats:styled-content>\n and joints with traumatic synovitis. Cut\u2010off values were set at 5000 and 350\u2009ng\/ml for total and active\n MPO<\/jats:styled-content>\n , respectively, with fair sensitivity, specificity, positive and negative predictive values and likelihood ratios for infection. Correlation coefficients were reported between the total as well as the active\n MPO<\/jats:styled-content>\n levels and the routine synovial fluid parameters, i.e. the white blood cell count, the neutrophil count and the total protein level. No correlation was observed between\n MPO<\/jats:styled-content>\n and either the age of the horse or the joint affected. Western blotting confirmed the antibody specificity for equine\n MPO<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions and potential relevance<\/jats:title>\n \n Synovial fluid\n MPO<\/jats:styled-content>\n was identified as a very promising biomarker to augment the discrimination of infectious vs. noninfectious joint disease in horses. Both\n ELISA<\/jats:styled-content>\n and\n SIEFED<\/jats:styled-content>\n techniques can be used for its specific and rapid detection. The analysis of synovial fluid\n MPO<\/jats:styled-content>\n can be used as a complementary test to aid in the discrimination between infectious and noninfectious joint disease, especially when the white blood cell counts and the total protein level are inconclusive.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/j.2042-3306.2012.00682.x","type":"journal-article","created":{"date-parts":[[2012,9,24]],"date-time":"2012-09-24T00:29:04Z","timestamp":1348446544000},"page":"278-283","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Equine myeloperoxidase: A novel biomarker in synovial fluid for the diagnosis of infection"],"prefix":"10.1111","volume":"45","author":[{"given":"J.","family":"Wauters","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Toxicology and Biochemistry Faculty of Veterinary Medicine Ghent University Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Pille","sequence":"additional","affiliation":[{"name":"Department of Surgery and Anaesthesiology of Domestic Animals Faculty of Veterinary Medicine Ghent University Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Martens","sequence":"additional","affiliation":[{"name":"Department of Surgery and Anaesthesiology of Domestic Animals Faculty of Veterinary Medicine Ghent University Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Franck","sequence":"additional","affiliation":[{"name":"Department of Large Animal Surgery Centre for Oxygen Research and Development University of Li\u00e8ge Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Serteyn","sequence":"additional","affiliation":[{"name":"Department of Large Animal Surgery Centre for Oxygen Research and Development University of Li\u00e8ge Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Gasthuys","sequence":"additional","affiliation":[{"name":"Department of Surgery and Anaesthesiology of Domestic Animals Faculty of Veterinary Medicine Ghent University Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Toxicology and Biochemistry Faculty of Veterinary Medicine Ghent University Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,11,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cveq.2005.08.001"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2005.07.019"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.2460\/javma.1991.198.09.1655","article-title":"Relations among synovial\u2010membrane histopathologic findings, synovial\u2010fluid cytologic findings, and bacterial culture results in horses with suspected infectious arthritis: 64 cases (1979\u20131987)","volume":"198","author":"Madison J.B.","year":"1991","journal-title":"J. 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