{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:25:07Z","timestamp":1792182307000,"version":"4.4.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Fatty acid metabolism is controlled not only by the acyl-coenzyme A (CoA) synthetases but by some enzymes in the \u03b2-oxidation cycle. Medium-chain and long-chain acyl-CoA esters are key metabolites in fatty acid metabolism. We have developed an enzymatic assay method for determining chain shortening of the acyl-CoAs via \u03b2-oxidation from palmitic and octanoic acids in liver mitochondria. We have evaluated the assay method for detecting whether drugs influence the activation or the \u03b2-oxidation of fatty acids.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Liver mitochondria were used for investigating the effect of drugs on fatty acid metabolism. The drugs selected were salicylic acid, diclofenac, valproic acid and paracetamol. Each acyl-CoA formed was analysed by liquid chromatography\u2013tandem mass spectrometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key findings<\/jats:title><jats:p>After less than 5 min of incubation, the levels of acyl-CoAs reflected the acyl-CoA synthetase activity, whereas after 60-min incubation they reflected the activity of some enzymes in the \u03b2-oxidation cycle. Salicylic acid, diclofenac and valproic acid inhibited the medium-chain acyl-CoA synthetases, whereas valproic acid only exhibited a weak inhibitory activity toward the \u03b2-oxidation of the medium-chain fatty acids. In the case of long-chain fatty acid metabolism, salicylic acid and diclofenac inhibited both the activation and \u03b2-oxidation, whereas valproic acid was a weak inhibitor for only the \u03b2-oxidation activity. Paracetamol showed hardly any influence on the metabolism of medium-chain and long-chain fatty acids.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings suggest that salicylic acid, diclofenac, valproic acid and paracetamol exert a different influence on fatty acid metabolism depending on the length of the acyl chain. This assay allows sensitive and selective analysis for predicting the pathways by which drugs exert a greater influence over fatty acid metabolism.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.2042-7158.2010.01136.x","type":"journal-article","created":{"date-parts":[[2010,10,4]],"date-time":"2010-10-04T21:01:39Z","timestamp":1286226099000},"page":"1697-1703","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of a screening method by liquid chromatography-tandem mass spectrometry for estimating effect of drugs on the activation and<i>\u03b2<\/i>-oxidation of fatty acids in mitochondria"],"prefix":"10.1093","volume":"62","author":[{"given":"Fumiyo","family":"Kasuya","sequence":"first","affiliation":[{"name":"Biochemical Toxicology Laboratory, Faculty of Pharmaceutical Sciences, Kobegakuin University , Kobe,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryota","family":"Nishizawa","sequence":"additional","affiliation":[{"name":"Biochemical Toxicology Laboratory, Faculty of Pharmaceutical Sciences, Kobegakuin University , Kobe,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teiichi","family":"Masuyama","sequence":"additional","affiliation":[{"name":"Biochemical Toxicology Laboratory, Faculty of Pharmaceutical Sciences, Kobegakuin University , Kobe,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya","family":"Kazumi","sequence":"additional","affiliation":[{"name":"Biochemical Toxicology Laboratory, Faculty of Pharmaceutical Sciences, Kobegakuin University , Kobe,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2010,12,1]]},"reference":[{"key":"2024103013095933100_cit1","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0074-7742(08)60306-2","article-title":"Excitotoxicity and neurological disorders: involvement of membrane 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