{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:02:59Z","timestamp":1792000979007,"version":"4.4.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>This study reports on the rapid isolation of verbascoside from Lippia citriodora H.B.K. (Verbenaceae), an inexpensive and widespread source, and the evaluation of its antihyperalgesic activity.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Isolation of verbascoside was achieved by size exclusion chromatography with Sephadex LH-20 eluting with 50% EtOH, which is proposed as a fast and efficient method of separation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>The antihyperalgesic activity of verbascoside was tested by in-vivo assay using the paw-pressure test in two animal models of neuropathic pain: a peripheral mononeuropathy produced either by a chronic constriction injury of the sciatic nerve (CCI) or by an intra-articular injection of sodium monoiodoacetate (MIA).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Verbascoside administered intraperitoneally at a dose of 100 mg\/kg reverted the mechanical hyperalgesia in both CCI and MIA treated rats, as evaluated in the paw-pressure test. Verbascoside was also effective against mechanical hyperalgesia after oral administration at doses of 300 and 600 mg\/kg.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/j.2042-7158.2011.01264.x","type":"journal-article","created":{"date-parts":[[2011,3,8]],"date-time":"2011-03-08T16:49:28Z","timestamp":1299602968000},"page":"594-601","source":"Crossref","is-referenced-by-count":41,"title":["Antihyperalgesic activity of verbascoside in two models of neuropathic pain"],"prefix":"10.1093","volume":"63","author":[{"given":"Benedetta","family":"Isacchi","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Florence , Sesto Fiorentino,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romina","family":"Iacopi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Florence , Sesto Fiorentino,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Camilla","family":"Bergonzi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Florence , Sesto Fiorentino,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Ghelardini","sequence":"additional","affiliation":[{"name":"Department of Preclinical and Clinical Pharmacology, University of Florence , Florence,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicoletta","family":"Galeotti","sequence":"additional","affiliation":[{"name":"Department of Preclinical and Clinical Pharmacology, University of Florence , Florence,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Norcini","sequence":"additional","affiliation":[{"name":"Department of Preclinical and Clinical Pharmacology, University of Florence , Florence,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Vivoli","sequence":"additional","affiliation":[{"name":"Department of Preclinical and Clinical Pharmacology, University of Florence , Florence,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco Francesco","family":"Vincieri","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Florence , Sesto Fiorentino,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Rita","family":"Bilia","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Florence , Sesto Fiorentino,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,4,1]]},"reference":[{"key":"2024103013292919100_cit1","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/0305-1978(92)90090-Z","article-title":"The distribution of acteoside among angiosperms","volume":"20","author":"Scogin","year":"1997","journal-title":"Biochem Syst Ecol"},{"key":"2024103013292919100_cit2","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1515\/znc-2007-7-810","article-title":"In vivo anti-inflammatory and antinociceptive activity evaluation of phenolic compounds from Sideritis stricta","volume":"62","author":"K\u00fcpeli","year":"2007","journal-title":"Naturforsch [C]"},{"key":"2024103013292919100_cit3","volume-title":"Xinhua Bencao Gangyao II","author":"Jiangshu Botanic Institute, Institute of Materia Medica CAMS, Kunming Botanic Institute","year":"1991"},{"key":"2024103013292919100_cit4","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1016\/j.lfs.2003.12.031","article-title":"Attenuated endothelium-mediated relaxation by acteoside in rat aorta: role of endothelial [Ca2+] and nitric oxide\/cyclic GMP pathway","volume":"75","author":"Lau","year":"2004","journal-title":"Life Sci"},{"key":"2024103013292919100_cit5","first-page":"84","article-title":"Molecular mechanisms underlying wound healing and anti-inflammatory properties of naturally occurring biotecnologically produced phenylpropanoid glycosides","volume":"30","author":"Korkina","year":"2007","journal-title":"Cell Mol Biol"},{"key":"2024103013292919100_cit6","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1111\/j.1365-2249.2007.03350.x","article-title":"In vivo treatment with herbal phenylethanoid acteoside ameliorates intestinal inflammation in dextran sulphate sodium-induced colitis","volume":"148","author":"Hausmann","year":"2007","journal-title":"Clin Exp Immunol"},{"key":"2024103013292919100_cit7","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.phymed.2006.05.006","article-title":"Antispasmodic activity of an extract from Plantago lanceolata L. and some isolated compounds","volume":"14","author":"Fleer","year":"2007","journal-title":"Phytomedicine"},{"key":"2024103013292919100_cit8","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.jep.2005.09.006","article-title":"Anti-inflammatory and anti-ulcerogenic properties of Stachytarpheta cayennensis (L.C. Rich) Valh","volume":"104","author":"Penido","year":"2006","journal-title":"J Ethnopharmacol"},{"key":"2024103013292919100_cit9","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.molmed.2007.07.001","article-title":"From traditional Chinese medicine to rational cancer therapy","volume":"13","author":"Efferth","year":"2007","journal-title":"Trends Mol Med"},{"key":"2024103013292919100_cit10","doi-asserted-by":"crossref","first-page":"1928","DOI":"10.1093\/carcin\/bgm126","article-title":"Acteoside inhibits human promyelocytic HL-60 leukemia cell proliferation via inducing cell cycle arret at G0\/G1 phase and differentiation into monocyte","volume":"28","author":"Lee","year":"2007","journal-title":"Carcinogenesis"},{"key":"2024103013292919100_cit11","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1002\/ptr.990","article-title":"In vitro immunomodulatory activity of verbascoside from Nepetya ucrainica L","volume":"16","author":"Akbay","year":"2002","journal-title":"Phytoter Res"},{"key":"2024103013292919100_cit12","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.jpba.2007.11.007","article-title":"HPLC\/DAD\/ESI-MS analysis of the constituents of aqueous preparations of verbena and lemon verbena and evaluation of the antioxidant activity","volume":"46","author":"Bilia","year":"2008","journal-title":"J Pharm Biomed Anal"},{"key":"2024103013292919100_cit13","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.jep.2005.01.005","article-title":"Inhibition of lipopolysaccharide-inducible nitric oxide synthase expression by acteoside trough blocking of AP-1 activation","volume":"97","author":"Lee","year":"2005","journal-title":"J Ethnopharmacol"},{"key":"2024103013292919100_cit14","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.fitote.2005.03.009","article-title":"Photoprotective activity of Buddleja scordioides","volume":"76","author":"Avila Acevedo","year":"2005","journal-title":"Fitoterapia"},{"key":"2024103013292919100_cit15","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.jep.2008.06.022","article-title":"Antinociceptive activity of Buddleja globosa (matico) in several models of pain","volume":"119","author":"Backhouse","year":"2008","journal-title":"J Ethnopharmacol"},{"key":"2024103013292919100_cit16","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.jep.2006.03.037","article-title":"Anti-inflammatory and analgesic activity of the topical preparation of Verbena officinalis L","volume":"107","author":"Calvo","year":"2006","journal-title":"J Ethnopharmacol"},{"key":"2024103013292919100_cit17","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1248\/cpb.45.499","article-title":"Acteoside as analgesic principle of Cedron (Lippia triphylla), a Peruvian medicinal plant","volume":"45","author":"Nakamura","year":"1997","journal-title":"Chem Pharm Bull"},{"key":"2024103013292919100_cit18","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1016\/S0140-6736(99)01307-0","article-title":"Neuropathic pain: aetiology, symptoms, mechanisms, and management","volume":"353","author":"Woolf","year":"1999","journal-title":"Lancet"},{"key":"2024103013292919100_cit19","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/S0304-3959(99)00154-2","article-title":"Efficacy of pharmacological treatments of neuropathic pain: an update and effect related to mechanism of drug action","volume":"83","author":"Syndrup","year":"1999","journal-title":"Pain"},{"key":"2024103013292919100_cit20","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/0014-2999(88)90390-1","article-title":"Intrathecal injection of a opioid agonist produces hyperalgesia in the guinea pig","volume":"157","author":"Leighton","year":"1988","journal-title":"Eur J Pharmacol"},{"key":"2024103013292919100_cit21","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/0304-3959(88)90209-6","article-title":"A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man","volume":"33","author":"Bennett","year":"1988","journal-title":"Pain"},{"key":"2024103013292919100_cit22","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.1002\/art.1780400917","article-title":"Mono-iodoacetate-induced experimental osteoarthritis: a dose-response study of loss of mobility, morphology, and biochemistry","volume":"40","author":"Guingamp","year":"1997","journal-title":"Arthritis Rheum"},{"key":"2024103013292919100_cit23","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1211\/jpp\/61.05.0012","article-title":"Pharmacological and metabolic characterisation of the potent sigma(1) receptor ligand 1\u2032-benzyl-3-methoxy-3H-spiro[[2]benzofuran-1,4\u2032-piperidine]","volume":"61","author":"Wiese","year":"2009","journal-title":"J Pharm Pharmacol"},{"key":"2024103013292919100_cit24","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1097\/00019052-199810000-00014","article-title":"Painful neuropathies","volume":"11","author":"Koltzenburg","year":"1998","journal-title":"Curr Opin Neurol"}],"container-title":["Journal of Pharmacy and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.2042-7158.2011.01264.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jpp\/article-pdf\/63\/4\/594\/60235480\/j.2042-7158.2011.01264.x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jpp\/article-pdf\/63\/4\/594\/60235480\/j.2042-7158.2011.01264.x.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T18:57:20Z","timestamp":1730314640000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jpp\/article\/63\/4\/594\/6135539"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,4,1]]},"references-count":24,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2011,4,1]]},"published-print":{"date-parts":[[2011,4,1]]}},"URL":"https:\/\/doi.org\/10.1111\/j.2042-7158.2011.01264.x","relation":{},"ISSN":["2042-7158","0022-3573"],"issn-type":[{"value":"2042-7158","type":"electronic"},{"value":"0022-3573","type":"print"}],"subject":[],"published-other":{"date-parts":[[2011,4]]},"published":{"date-parts":[[2011,4,1]]}}}