{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T03:13:49Z","timestamp":1784862829666,"version":"3.55.0"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study investigated whether spinosin potentiates pentobarbital-induced loss of righting reflex (LORR) in mice via 5-HT1A receptors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Our primary endpoint for sedation was LORR. In addition, the basal rectal temperature was measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key findings<\/jats:title><jats:p>The results demonstrated that the 5-HT1A agonist 8-OH-DPAT (s.c.) induced reductions in duration of LORR at 0.1, 0.5 and 1.0 mg\/kg (P &amp;lt; 0.01), and prolongation of LORR latency at 0.5 and 1.0 mg\/kg (s.c., P &amp;lt; 0.01) in pentobarbital (45 mg\/kg, i.p.)-treated mice. This effect of 8-OH-DPAT was antagonized either by 5-HT1A antagonist p-MPPI (5 mg\/kg, i.p.) or by spinosin (15 mg\/kg, i.g.) with significance, respectively. Co-administration of spinosin and p-MPPI both at ineffective doses (spinosin at 5.0 mg\/kg, i.g. and p-MPPI at 1.0 mg\/kg, i.p.) showed significant augmentative effects in reducing latency to LORR, and increasing LORR duration (P &amp;lt; 0.01) in pentobarbital-treated mice. On the other hand, spinosin inhibited 8-OH-DPAT-induced hypothermia, which has been generally attributed to the activation of somatodendritic 5-HT1A autoreceptors in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Based on our previous results and the present data, it should be presumed that presynaptic 5-HT1A autoreceptor mechanisms may be involved in the inhibitory effect of spinosin on 8-OH-DPAT-induced hypothermia and also in the potentiating effect of spinosin on pentobarbital-induced LORR in mice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.2042-7158.2011.01400.x","type":"journal-article","created":{"date-parts":[[2011,11,18]],"date-time":"2011-11-18T10:02:32Z","timestamp":1321610552000},"page":"277-282","source":"Crossref","is-referenced-by-count":26,"title":["Augmentative effect of spinosin on pentobarbital-induced loss of righting reflex in mice associated with presynaptic 5-HT1A receptor"],"prefix":"10.1093","volume":"64","author":[{"given":"Li-En","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Science, Peking University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Qiong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Science, Peking University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Qi","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Science, Peking University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-He","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Science, Peking University , 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