{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,14]],"date-time":"2026-11-14T11:33:59Z","timestamp":1794656039608,"version":"4.9.1"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>We investigated the effect of Cistanche deserticola Ma. (CD) on bone formation by cultured osteoblasts.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The mineralized nodule formation assay was used to examine the in-vitro effects of CD on bone formation. Alkaline phosphatase (ALP), bone morphogenetic proteins (BMP)-2 and osteopontin (OPN) mRNA expression was analysed by quantitative real-time polymerase chain reaction. The mechanism of action of CD extract was investigated using Western blotting. The in-vivo anti-osteoporotic effect of CD extract was assessed in ovariectomized mice.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>CD extract had no effect on the proliferation, migration or wound healing of cultured osteoblasts, but increased ALP, BMP-2 and OPN mRNA and bone mineralization. Mitogen-activated protein kinase (MAPK) or nuclear factor (NF)-\u03baB inhibitors reduced CD extract-induced bone formation and ALP, BMP-2 and OPN expression. However, CD extract did not affect osteoclastogenesis. In addition, CD extract prevented the bone loss induced by ovariectomy in vivo.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>CD may be a novel bone formation agent for the treatment of osteoporosis.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/j.2042-7158.2012.01483.x","type":"journal-article","created":{"date-parts":[[2012,3,8]],"date-time":"2012-03-08T09:13:42Z","timestamp":1331198022000},"page":"897-907","source":"Crossref","is-referenced-by-count":37,"title":["<i>Cistanche deserticola<\/i> extract increases bone formation in osteoblasts"],"prefix":"10.1093","volume":"64","author":[{"given":"Te-Mao","family":"Li","sequence":"first","affiliation":[{"name":"School of Chinese Medicine , Tainan,","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin-Chih","family":"Huang","sequence":"additional","affiliation":[{"name":"Chi-Mei Medical Center , Tainan,","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen-Ming","family":"Su","sequence":"additional","affiliation":[{"name":"Graduate Institute of Basic Medical Science, China Medical University Hospital , Taichung"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tin-Yun","family":"Ho","sequence":"additional","affiliation":[{"name":"School of Chinese Medicine , Tainan,","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Ming","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Chinese Medicine , Tainan,","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Chi","family":"Chen","sequence":"additional","affiliation":[{"name":"Graduate Institute of Integrated Medicine, China Medical University Hospital , Taichung"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Chin","family":"Fong","sequence":"additional","affiliation":[{"name":"School of Chinese Medicine , Tainan,","place":["Taiwan"]},{"name":"Department of Orthopaedics, China Medical University Hospital , Taichung"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Hsin","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, China Medical University"},{"name":"Graduate Institute of Basic Medical Science, China Medical University Hospital , Taichung"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,6,1]]},"reference":[{"key":"2024103013323040900_cit1","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1046\/j.1365-2567.2001.01261.x","article-title":"Nitric oxide and bone","volume":"3","author":"van't 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