{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T01:43:52Z","timestamp":1789177432248,"version":"build-2803163510"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The aim of this study was to determine whether diacylglycerol kinase (DGK) is involved in transplasmalemmal Ca2+ influx of platelets.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Effects of R59949, an inhibitor of diacylglycerol kinase, on intracellular Ca2+ concentration ([Ca2+]i) and mRNA expression of DGK isozymes were investigated using washed human platelet suspensions.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>Thrombin-induced increase in [Ca2+]i was significantly inhibited by pretreatment of platelets with R59949, while thapsigargin-induced increase in [Ca2+]i was comparable in platelets with and without R59949 pretreatment. Thapsigargin-induced increase in [Ca2+]i was markedly attenuated in the presence of SKF-96365. In the presence of SKF-96365, thrombin-induced increase in [Ca2+]i was significantly attenuated, and additional treatment with R59949 caused a further decrease in [Ca2+]i. Pretreatment of platelets with 1-butanol significantly attenuated thrombin-induced increase in [Ca2+]i, while thrombin-induced increase in [Ca2+]i was augmented in the presence of propranolol. mRNA expression of DGK-\u03b1 and DGK-\u03b3, which are known to be inhibited by R59949, in platelets was confirmed by RT-PCR analysis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>R59949 inhibited a store-depletion-insensitive component of transplasmalemmal Ca2+ entry induced by thrombin, while store-operated Ca2+ entry was not affected by R59949. The results of this study suggest that phosphatidic acid is involved in thrombin-induced Ca2+ influx of platelets.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/j.2042-7158.2012.01485.x","type":"journal-article","created":{"date-parts":[[2012,3,12]],"date-time":"2012-03-12T11:42:33Z","timestamp":1331552553000},"page":"855-861","source":"Crossref","is-referenced-by-count":12,"title":["Inhibition of thrombin-induced Ca2+ influx in platelets by R59949, an inhibitor of diacylglycerol kinase"],"prefix":"10.1093","volume":"64","author":[{"given":"Mikio","family":"Marumo","sequence":"first","affiliation":[{"name":"Department of Environmental and Preventive Medicine, Hyogo College of Medicine , Mukogawa-cho, Nishinomiya, Hyogo,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyuki","family":"Nakano","sequence":"additional","affiliation":[{"name":"Department of Environmental and Preventive Medicine, Hyogo College of Medicine , Mukogawa-cho, Nishinomiya, Hyogo,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Takeda","sequence":"additional","affiliation":[{"name":"Department of Environmental and Preventive Medicine, Hyogo College of Medicine , Mukogawa-cho, Nishinomiya, Hyogo,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaoru","family":"Goto","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Yamagata University School of Medicine , Iida-Nishi, Yamagata,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Wakabayashi","sequence":"additional","affiliation":[{"name":"Department of Environmental and Preventive Medicine, Hyogo College of Medicine , Mukogawa-cho, Nishinomiya, Hyogo,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,6,1]]},"reference":[{"key":"2024103013322919500_cit1","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1016\/j.bbalip.2007.04.006","article-title":"Diacylglycerol kinases: why so many of them?","volume":"1771","author":"Sakane","year":"2007","journal-title":"Biochim Biophys Acta"},{"key":"2024103013322919500_cit2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0074-7696(07)64002-9","article-title":"Cell biology and pathophysiology of the diacylglycerol kinase family: morphological aspects in tissues and organs","volume":"264","author":"Goto","year":"2007","journal-title":"Int Rev Cytol"},{"key":"2024103013322919500_cit3","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.advenzreg.2003.11.007","article-title":"Functional implications of the diacylglycerol kinase family","volume":"44","author":"Goto","year":"2004","journal-title":"Adv Enzyme Regul"},{"key":"2024103013322919500_cit4","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1152\/physrev.1989.69.1.58","article-title":"Molecular mechanisms of platelet activation","volume":"69","author":"Siess","year":"1989","journal-title":"Physiol Rev"},{"key":"2024103013322919500_cit5","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/978-3-540-34891-7_25","article-title":"TRP channels in platelet function","volume":"179","author":"Authi","year":"2007","journal-title":"Handb Exp Pharmacol"},{"key":"2024103013322919500_cit6","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/S0006-2952(99)00395-0","article-title":"Selectivity of the diacylglycerol kinase inhibitor 3-[2-(4-[bis-(4-fluorophenyl)methylene]-1-piperidinyl)ethyl]-2, 3-dihydro-2-thioxo-4(1H)quinazolinone (R59949) among diacylglycerol kinase subtypes","volume":"59","author":"Jiang","year":"2000","journal-title":"Biochem Pharmacol"},{"key":"2024103013322919500_cit7","doi-asserted-by":"crossref","first-page":"3274","DOI":"10.1016\/S0021-9258(18)94063-9","article-title":"The role of endogenously formed diacylglycerol in the propagation and termination of platelet activation. 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