{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T23:45:27Z","timestamp":1794354327611,"version":"4.9.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To evaluate the effect of \u03b2-sitosterol on 45Ca2+ uptake in activated murine neutrophils, and upon myeloperoxidase and adenosine deaminase activity, and interleukin-1\u03b2 (IL-1\u03b2) and tumour necrosis factor-\u03b1 (TNF-\u03b1) levels, in carrageenan-induced inflammation in the mouse air pouch model.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Dried Esenbeckia leiocarpa bark was macerated and extracted resulting in a crude hydroalcoholic extract (CHE) that was partitioned to obtain an alkaloid fraction. The alkaloid was then partitioned in polar and nonpolar subfractions. \u03b2-Sitosterol was isolated from the nonpolar subfraction and identified by comparison with the literature. The effect of \u03b2-sitosterol on 45Ca2+ uptake in activated murine neutrophils, and upon myeloperoxidase and adenosine deaminase activity, IL-1\u03b2 and TNF-\u03b1 levels in carrageenan-induced inflammation in mice were evaluated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>\u03b2-Sitosterol promoted a time- and dose-dependent increase of the calcium uptake in activated neutrophils that was promptly reversed by nifedipine, BAPTA-AM, LY294002, and colchicine. \u03b2-Sitosterol inhibited myeloperoxidase and adenosine deaminase activity, and IL-1\u03b2 and TNF-\u03b1 levels.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>\u03b2-Sitosterol inhibited either myeloperoxidase and adenosine deaminase activity or IL-1\u03b2 and TNF-\u03b1 levels. This effect seemed to be mediated by the calcium uptake in activated neutrophils in a time- and concentration-dependent manner through L-type voltage dependent calcium channels, intracellular calcium, phosphoinositide kinase-3, and microtubule modulation.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/j.2042-7158.2012.01568.x","type":"journal-article","created":{"date-parts":[[2012,10,11]],"date-time":"2012-10-11T13:29:52Z","timestamp":1349962192000},"page":"115-122","source":"Crossref","is-referenced-by-count":34,"title":["Acute effect of \u03b2-sitosterol on calcium uptake mediates anti-inflammatory effect in murine activated neutrophils"],"prefix":"10.1093","volume":"65","author":[{"given":"Rafael","family":"Liz","sequence":"first","affiliation":[{"name":"Department of Clinical Analysis, Centre of Health Sciences , Florian\u00f3polis,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Zanatta","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Centre of Biological Sciences , Florian\u00f3polis,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Oliveira","family":"dos Reis","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, Centre of Health Sciences , Florian\u00f3polis,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heros","family":"Horst","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Centre of Physical and Mathematical Sciences, Federal University of Santa Catarina , Florian\u00f3polis,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moacir Geraldo","family":"Pizzolatti","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Centre of Physical and Mathematical Sciences, Federal University of Santa Catarina , Florian\u00f3polis,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1tima Regina Mena Barreto","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Centre of Biological Sciences , Florian\u00f3polis,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00e2nia Silvia","family":"Fr\u00f6de","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, Centre of Health Sciences , Florian\u00f3polis,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,1,1]]},"reference":[{"key":"2024103109161343400_cit1","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1016\/j.intimp.2010.08.012","article-title":"Neutrophils: cinderella of innate immune 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