{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T17:36:19Z","timestamp":1774546579836,"version":"3.50.1"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Scientific and Research Council of Turkey","award":["TBAG-104T406"],"award-info":[{"award-number":["TBAG-104T406"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Two new metal complexes, diaquabis(4-benzoyl-1,5-diphenyl-N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)-1H-pyrazole-3-carboxamide)cobalt(II) dihydrate (2) and diaquabis(ethyl-1-(3-nitrophenyl)-5-phenyl-3-(5-sulfamoyl-1,3,4-thiadiazol-2-ylcarbamoyl)-1H-pyrazole-4-carboxylate)cobalt(II) monohydrate (4), containing sulfonamide have been synthesized by the reaction of Co(II) with 4-benzoyl-1,5-diphenyl-N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)-1H-pyrazole-3-carboxamide (1) and ethyl-1-(3-nitrophenyl)-5-phenyl-3-(5-sulfamoyl-1,3,4-thiadiazol-2-ylcarbamoyl)-1H-pyrazole-4-carboxylate (3), respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The structures of Co(II) complexes 2 and 4 have been characterised by spectroscopic methods and elemental analyses. Human carbonic anhydrase isoenzymes (hCA-I and hCA-II) were purified from erythrocyte cells by affinity chromatography. The inhibitory effects of ligands 3 and 4, acetazolamide as a control compound and the newly synthesized complexes on the activity of hydratase and esterase of these isoenzymes have been studied in vitro.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>The concentration of compounds 2 and 4 producing a 50% inhibition of hydratase activity (IC50 values) were 0.473 \u00b1 0.025 and 0.065 \u00b1 0.002 \u03bcm for hCA-I and 0.213 \u00b1 0.015 and 0.833 \u00b1 0.021 \u03bcm for hCA-II, respectively. The IC50 values of synthesized compounds 2 and 4 for esterase activity were, 0.058 \u00b1 0.006 and 0.297 \u00b1 0.015 \u03bcm for hCA-I and 0.110 \u00b1 0.010 and 0.052 \u00b1 0.002 \u03bcm for hCA-II, respectively. In relation to esterase activity, the inhibition equilibrium constants (Ki) were determined as 0.039 \u00b1 0.004 and 0.247 \u00b1 0.035 \u03bcm on hCA-I and 0.078 \u00b1 0.002 and 0.363 \u00b1 0.015 \u03bcm on hCA-II for 2 and 4, respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The synthesized compounds 2 and 4 had effective inhibitory activity (P &amp;lt; 0.0001) on hCA-I and hCA-II than the corresponding free ligands, 1 and 3, and acetazolamide. Compounds 2 and 4 might be considered as potential inhibitors.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/j.2042-7158.2012.01609.x","type":"journal-article","created":{"date-parts":[[2012,11,16]],"date-time":"2012-11-16T13:19:39Z","timestamp":1353071979000},"page":"363-369","source":"Crossref","is-referenced-by-count":6,"title":["Synthesis and characterisation of novel Co(II) complexes of pyrazole carboxylate derivated of sulfonamide as carbonic anhydrase inhibitors"],"prefix":"10.1093","volume":"65","author":[{"given":"Nurg\u00fcn","family":"B\u00fcy\u00fckkidan","sequence":"first","affiliation":[{"name":"Department of Chemistry, Arts and Science Faculty, Dumlupinar University , K\u00fctahya,","place":["Turkey"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00fclent","family":"B\u00fcy\u00fckkidan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Arts and Science Faculty, Dumlupinar University , K\u00fctahya,","place":["Turkey"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Metin","family":"B\u00fclb\u00fcl","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Arts and Science Faculty, Dumlupinar University , K\u00fctahya,","place":["Turkey"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahmi","family":"Kasimo\u011fullari","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Arts and Science Faculty, Dumlupinar University , K\u00fctahya,","place":["Turkey"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murat","family":"Serdar","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Arts and Science Faculty, Dumlupinar University , K\u00fctahya,","place":["Turkey"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samet","family":"Mert","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Arts and Science Faculty, Dumlupinar University , K\u00fctahya,","place":["Turkey"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,3,1]]},"reference":[{"key":"2024103109145919300_jphp1609-cit-0001","doi-asserted-by":"crossref","first-page":"625","DOI":"10.2174\/1389557043403792","article-title":"COX-2 selective inhibitors, carbonic anhydrase inhibition and anticancer properties of sulfonamides belonging to this class of pharmacological agents","volume":"4","author":"Supuran","year":"2004","journal-title":"Mini Rev Med Chem"},{"key":"2024103109145919300_jphp1609-cit-0002","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1002\/med.10025","article-title":"Carbonic anhydrase inhibitors","volume":"23","author":"Supuran","year":"2003","journal-title":"Med Res Rev"},{"key":"2024103109145919300_jphp1609-cit-0003","first-page":"243","article-title":"Clinical applications of the carbonic anhydrase inhibitors in ophthalmology","author":"Mincione","year":"2004"},{"key":"2024103109145919300_jphp1609-cit-0004","first-page":"443","article-title":"Carbonic anhydrase inhibitors: inhibition of isozymes I, II and IV by sulfamide and sulfamic acid derivatives","volume":"15","author":"Scozzafava","year":"2000","journal-title":"J Enzyme Inhib Med Chem"},{"key":"2024103109145919300_jphp1609-cit-0005","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0149-2918(00)88478-7","article-title":"Antiglaucoma medications: a review of safety and tolerability issues related to their use","volume":"22","author":"Schuman","year":"2000","journal-title":"Clin Ther"},{"key":"2024103109145919300_jphp1609-cit-0006","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1016\/j.bmcl.2006.11.046","article-title":"Inhibition of membrane-associated carbonic anhydrase isozymes IX, XII and XIV with a library of glycoconjugate benzenesulfonamides","volume":"17","author":"Wilkinson","year":"2007","journal-title":"Bioorg Med Chem Lett"},{"key":"2024103109145919300_jphp1609-cit-0007","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.1016\/j.bmcl.2006.12.107","article-title":"Carbonic anhydrase inhibitors: inhibition of cytosolic\/tumor associated isoforms I, II, and IX with iminodiacetic carboxylates\/hydroxamates also incorporating benzene sulfonamide moieties","volume":"17","author":"Santos","year":"2007","journal-title":"Bioorg Med Chem Lett"},{"key":"2024103109145919300_jphp1609-cit-0008","first-page":"739","article-title":"I.Carbonic anhydrase inhibitors. 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