{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:16:21Z","timestamp":1758125781556},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,6,28]],"date-time":"2008-06-28T00:00:00Z","timestamp":1214611200000},"content-version":"vor","delay-in-days":2980,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:p>PROBLEM: To determine whether leptin exhibits cytokine\u2010like properties in gestational tissues in light of its homologies with the class I family of cytokines.\u2028 METHOD OF STUDY: WISH and JEG3 cells, and amnion and choriodecidua explants, were treated inflammatory modulators (interleukin\u20101\u03b2 [IL\u20101\u03b2], tumor necrosis factor\u2010\u03b1 [TNF\u2010\u03b1] and bacterial lipopolysaccharide [LPS]) and leptin production was measured by immunoassay. Other agents known to regulate adipocyte leptin production were also tested for comparative purposes. In addition, WISH cells, JAR cells and placental explants were treated with leptin to assess its effects on production of IL\u20108, IL\u20106 and prostaglandin E<jats:sub>2<\/jats:sub> \n(PGE<jats:sub>2<\/jats:sub>).\u2028\nRESULTS: Leptin production by all cells and tissues studied was \nunaffected by treatment with IL\u20101\u03b2 (2.5 ng\/mL), TNF\u2010\u03b1 (25 \nng\/mL) and LPS (2.5 \u03bcg\/mL). Dexamethasone stimulated leptin production \nover two\u2010fold by WISH and JEG3 cells, whereas insulin also stimulated a \ntwo\u2010fold increase in leptin production in JEG3 cells. IL\u20106 production by \nJAR cells and placental explants was stimulated (two\u2010 to three\u2010fold) by \nleptin (300 ng\/mL). PGE<jats:sub>2<\/jats:sub> production was unaffected.\u2028 CONCLUSIONS: Leptin derived from gestational tissues is unlikely to play a role in inflammatory reactions within the placenta, but may regulate placental cytokine production. The physiological significance of amnion\u2010derived leptin remains to be established.<\/jats:p>","DOI":"10.1111\/j.8755-8920.2000.430508.x","type":"journal-article","created":{"date-parts":[[2003,12,17]],"date-time":"2003-12-17T11:50:24Z","timestamp":1071661824000},"page":"292-298","source":"Crossref","is-referenced-by-count":12,"title":["Does Leptin Exhibit Cytokine\u2010Like Properties in Tissues of Pregnancy?"],"prefix":"10.1111","volume":"43","author":[{"given":"E.B.E.","family":"SOH","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.D.","family":"MITCHELL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.A.","family":"KEELAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,6,28]]},"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.8755-8920.2000.430508.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.8755-8920.2000.430508.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.8755-8920.2000.430508.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T09:37:41Z","timestamp":1698572261000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.8755-8920.2000.430508.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,5]]}},"alternative-id":["10.1111\/j.8755-8920.2000.430508.x"],"URL":"https:\/\/doi.org\/10.1111\/j.8755-8920.2000.430508.x","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,5]]}}}