{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T23:17:35Z","timestamp":1767827855903,"version":"3.49.0"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,6,28]],"date-time":"2008-06-28T00:00:00Z","timestamp":1214611200000},"content-version":"vor","delay-in-days":2888,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2000,8]]},"abstract":"<jats:p>PROBLEM: Neonatal estradiol injections in mice lead to follicular cystic ovaries that are similar to ovaries in patients with polycystic ovarian syndrome (PCOS). The present study examined ovarian cytokine production following neonatal estradiol injection.\u2028 METHOD OF STUDY: Female (C3H\/HeJ\u00d7129\/HeJ)F<jats:sub>1<\/jats:sub> mice were injected daily with 20 \u03bcg 17\u03b2\u2010estradiol from 0\u20133 days postpartum. At intervals, animals were sacrificed to determine ovarian architecture, circulating levels of estradiol, ovarian and peritoneal machrophage cytokine production, and ovarian P450 aromatase enzyme mRNA levels.\u2028 RESULTS: Similar to PCOS, our results show that neonatally estradiol\u2010injected mice have lower levels of circulating estrogen that are correlated with decreased mRNA levels of P450 aromatase enzyme. Our data also show that follicular cystic ovaries have increased tumor necrosis factor (TNF)\u2010\u03b1 and interleukin (IL)\u20106 production. This increase in TNF\u2010\u03b1 and IL\u20106 production is also observed in peritoneal macrophages of estradiol\u2010injected mice.\u2028 CONCLUSION: The present study showed that neonatal estrogen injection in mice has an overall systemic effect on cytokine production. We speculate that increased cytokine production may alter certain important steps in follicular maturation, ultimately contributing to ovarian dysfunction.<\/jats:p>","DOI":"10.1111\/j.8755-8920.2000.440203.x","type":"journal-article","created":{"date-parts":[[2003,12,17]],"date-time":"2003-12-17T11:56:46Z","timestamp":1071662206000},"page":"80-88","source":"Crossref","is-referenced-by-count":31,"title":["Alteration of Cytokine Production in Follicular Cystic Ovaries Induced in Mice by Neonatal Estradiol Injection"],"prefix":"10.1111","volume":"44","author":[{"given":"ROHINI R.","family":"DESHPANDE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JOHN C.","family":"CHAPMAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SANDRA D.","family":"MICHAEL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ROHINI R.","family":"DESHPANDE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MING\u2010DER Y.","family":"CHANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,6,28]]},"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.8755-8920.2000.440203.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.8755-8920.2000.440203.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.8755-8920.2000.440203.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T17:15:17Z","timestamp":1698599717000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.8755-8920.2000.440203.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,8]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2000,8]]}},"alternative-id":["10.1111\/j.8755-8920.2000.440203.x"],"URL":"https:\/\/doi.org\/10.1111\/j.8755-8920.2000.440203.x","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,8]]}}}