{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T08:29:40Z","timestamp":1777537780631,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,6,28]],"date-time":"2008-06-28T00:00:00Z","timestamp":1214611200000},"content-version":"vor","delay-in-days":2766,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2000,12]]},"abstract":"<jats:p>PROBLEM: Chemokine receptors of placental trophoblasts possibly act as co\u2010receptors or alternative receptors of maternal\u2013fetal infection by HIV. To clarify their possible expression and the physiological roles of chemokines on human placentae, we studied chemokine\/chemokine receptor expression and the effects of exogenous chemokines on choriocarcinoma cell lines.\u2028 MATERIALS AND METHODS: Placental samples were obtained from 13 placentae of various gestational ages. Villous tissue was mechanically dissected from samples. Trophoblasts were enriched by anti\u2010human chorionic gonadotropin (hCG)\u2010coated magnetic beads. Human choriocarcinoma cell lines (JAR, BeWo, JEG\u20103) were maintained in RPMI 1640 media supplemented with 10% FCS. Expression of chemokine receptors was studied by RT\u2010PCR. The effects of MIP\u20101\u03b1, RANTES, MCP\u20101 on hCG production were estimated by EIA. Effects of chemokines on proliferation of choriocarcinoma cell lines were examined by MTT assay.\u2028 RESULTS: We observed mRNA expression of CCR\u20101, 2, 3, 4, 5 and CXCR\u20101, 2, 4 in 1st trimester placental villi, CCR\u20101, 2, 4 and CXCR\u20101, 2, 4 in 2nd trimester placental villi, CCR\u20101, 2, 4 and CXCR\u20104 in 3rd trimester placental villi. Using MACS enriched trophoblasts, we observed identical results. A choriocarcinoma cell line BeWo expressed CCR\u20101, 3, 4 and CXCR\u20101, 2, 4 while JEG\u20103 and JAR expressed CCR\u20101, 3, 4, 5 and CXCR\u20101, 2, 4. Expression of the CCR\u20105 and CXCR\u20104 protein in choriocarcinoma cell lines and MACS\u2010enriched trophoblats were confirmed by flow cytometry. Chemokine MCP\u20103, MIP\u20101\u03b1, RANTES mRNA were expressed by the 1st, 2nd and 3rd trimester placental samples and the three choriocarcinoma cell lines examined. MCP\u20101 was expressed by 1st and 2nd trimester placental villi. Administration of chemokines up\u2010regulated proliferation (10\u22121\u201310 ng\/mL) and hCG production (10\u22121\u201310\u22122ng\/mL) of the three choriocarcinoma cell lines examined.\u2028 CONCLUSIONS: Our results suggest possible roles of chemokines\/chemokine receptors on placental physiology and their involvement in HIV transmission as alternative receptors.<\/jats:p>","DOI":"10.1111\/j.8755-8920.2000.440608.x","type":"journal-article","created":{"date-parts":[[2003,12,17]],"date-time":"2003-12-17T11:55:09Z","timestamp":1071662109000},"page":"365-373","source":"Crossref","is-referenced-by-count":34,"title":["Expression of Functional Chemokine Receptors of Human Placental Cells"],"prefix":"10.1111","volume":"44","author":[{"given":"MAKI","family":"ISHII","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SATOSHI","family":"HAYAKAWA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MIKI\u2010KARASAKI","family":"\u2010SUZUKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"FUMIHISA","family":"CHISHIMA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MASAJI","family":"NAGAISHI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KAZUO","family":"SATOH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SATOSHI","family":"HAYAKAWA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"NAOTO","family":"YOSHINO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MITSUO","family":"HONDA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SUSUMU","family":"NISHINARITA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,6,28]]},"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.8755-8920.2000.440608.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.8755-8920.2000.440608.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.8755-8920.2000.440608.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T08:44:22Z","timestamp":1698569062000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.8755-8920.2000.440608.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2000,12]]}},"alternative-id":["10.1111\/j.8755-8920.2000.440608.x"],"URL":"https:\/\/doi.org\/10.1111\/j.8755-8920.2000.440608.x","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,12]]}}}