{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T04:48:13Z","timestamp":1769834893672,"version":"3.49.0"},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,12,17]],"date-time":"2003-12-17T00:00:00Z","timestamp":1071619200000},"content-version":"vor","delay-in-days":990,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2001,4]]},"abstract":"<jats:p>PROBLEM: The dynamics of calpain and involvement of apoptosis in sperm formation disorder of the unaffected testis in rats with experimental testicular torsion were investigated.\u2028 METHODS OF STUDY: Using 6\u2010week\u2010old Wistar rats, an experimental unilateral testicular torsion model was prepared. The bilateral testes were excised 1, 3, 5, 7, 14, 35, and 70 days after the left testis was twisted, and the unaffected testes were subjected to immunohistological staining, sodium dodecyl sulfate polyacrilamide gel electrophoresis and Western blotting using anti\u2010calpain antibodies. Apoptosis was detected by the TdT\u2010mediated dUTP\u2013biotin nick end\u2010labeling (TUNEL) method.\u2028 RESULTS: By immunohistological staining, positive immunostaining by anti\u2010pro \u03bc\u2010calpain antibody was observed in the spermatocyte nucleus, but not with anti\u2010pro m\u2010calpain antibody. The staining was increased until 7 days after testicular torsion, then decreased with progression of sperm formation disorder. By Western blotting, the intensity of staining with anti\u2010pro \u03bc\u2010calpain antibody was increased until 7 days after torsion of the testis. Apoptosis expression in the unaffected testis was significantly inhibited by addition of a calpain inhibitor.\u2028 CONCLUSIONS: It was suggested that \u03bc\u2010calpain may be involved in apoptosis expression in sperm formation disorder of the unaffected testis in unilateral testicular torsion.<\/jats:p>","DOI":"10.1111\/j.8755-8920.2001.450407.x","type":"journal-article","created":{"date-parts":[[2003,12,17]],"date-time":"2003-12-17T11:58:13Z","timestamp":1071662293000},"page":"239-245","source":"Crossref","is-referenced-by-count":7,"title":["Involvement of Calpain for Apoptosis in Dysfunction of the Unaffected Testis in Rats with Experimental Testicular Torsion"],"prefix":"10.1111","volume":"45","author":[{"given":"YUKIHIRO","family":"UMEMOTO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SHOICHI","family":"SASAKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HIROYUKI","family":"TATSURA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HIROKI","family":"KUBOTA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YASUE","family":"KUBOTA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KENJIRO","family":"KOHRI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YASUHIKO","family":"OZAKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MAKOTO","family":"SASAKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YASUHIKO","family":"OZAKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,12,17]]},"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.8755-8920.2001.450407.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.8755-8920.2001.450407.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T17:30:31Z","timestamp":1700415031000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.8755-8920.2001.450407.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,4]]}},"alternative-id":["10.1111\/j.8755-8920.2001.450407.x"],"URL":"https:\/\/doi.org\/10.1111\/j.8755-8920.2001.450407.x","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,4]]}}}