{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:54:18Z","timestamp":1791194058864,"version":"4.1.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2014,4,29]],"date-time":"2014-04-29T00:00:00Z","timestamp":1398729600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2014,4,29]],"date-time":"2014-04-29T00:00:00Z","timestamp":1398729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"MCT\/CNPq","award":["15\/2007"],"award-info":[{"award-number":["15\/2007"]}]},{"DOI":"10.13039\/501100006162","name":"FACEPE","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006162","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Appl Microbiol"],"published-print":{"date-parts":[[2014,7]]},"DOI":"10.1111\/jam.12508","type":"journal-article","created":{"date-parts":[[2014,3,29]],"date-time":"2014-03-29T02:42:47Z","timestamp":1396060967000},"page":"266-272","source":"Crossref","is-referenced-by-count":11,"title":["Assessment of messenger RNA (mRNA) of\n                    <i>Mycobacterium tuberculosis<\/i>\n                    as a marker of cure in patients with pulmonary tuberculosis"],"prefix":"10.1093","volume":"117","author":[{"given":"R.A.","family":"Montenegro","sequence":"first","affiliation":[{"name":"Department of Immunology; Aggeu Magalh\u00e3es Research Center - Fiocruz; Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.M.","family":"Guarines","sequence":"additional","affiliation":[{"name":"Department of Immunology; Aggeu Magalh\u00e3es Research Center - Fiocruz; Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.M.L.","family":"Montenegro","sequence":"additional","affiliation":[{"name":"Department of Immunology; Aggeu Magalh\u00e3es Research Center - Fiocruz; Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.A.S.","family":"Lira","sequence":"additional","affiliation":[{"name":"Department of Immunology; Aggeu Magalh\u00e3es Research Center - Fiocruz; Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Falc\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Immunology; Aggeu Magalh\u00e3es Research Center - Fiocruz; Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.L.","family":"Melo","sequence":"additional","affiliation":[{"name":"Department of Immunology; Aggeu Magalh\u00e3es Research Center - Fiocruz; Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.C.F.","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Immunology; Aggeu Magalh\u00e3es Research Center - Fiocruz; Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.L.A.","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Department of Immunology; Aggeu Magalh\u00e3es Research Center - Fiocruz; Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.S.","family":"Zuzarte","sequence":"additional","affiliation":[{"name":"Department of Immunology; Aggeu Magalh\u00e3es Research Center - Fiocruz; Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.C.","family":"Leite","sequence":"additional","affiliation":[{"name":"Department of Immunology; Aggeu Magalh\u00e3es Research Center - Fiocruz; Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.C.","family":"Schindler","sequence":"additional","affiliation":[{"name":"Department of Immunology; Aggeu Magalh\u00e3es Research Center - Fiocruz; Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,4,29]]},"reference":[{"key":"10.1111\/jam.12508-BIB0001|jam12508-cit-0001","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1590\/S1806-37132010000500016","article-title":"Antituberculosis drugs: drug interactions, adverse effects, and use in special situations - part 1: first-line drugs","volume":"36","author":"Arbex","year":"2010","journal-title":"J Bras Pneumol"},{"key":"10.1111\/jam.12508-BIB0002|jam12508-cit-0002","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1097\/PDM.0b013e31814ceac3","article-title":"Real-time quantitative PCR in the diagnosis of tuberculosis in formalin-fixed paraffin-embedded pleural tissue in patients from a high HIV endemic area","volume":"17","author":"Baba","year":"2008","journal-title":"Diagn Mol Pathol"},{"key":"10.1111\/jam.12508-BIB0005|jam12508-cit-0005","doi-asserted-by":"crossref","first-page":"2644","DOI":"10.1128\/JCM.42.6.2644-2650.2004","article-title":"Real-time PCR assay using fine-needle aspirates and tissue biopsy specimens for rapid diagnosis of mycobacterial lymphadenitis in children","volume":"42","author":"Bruijnesteijn Van Coppenraet","year":"2004","journal-title":"J Clin Microbiol"},{"key":"10.1111\/jam.12508-BIB0501|jam12508-cit-0501","doi-asserted-by":"crossref","first-page":"S57","DOI":"10.1590\/S1806-37132004000700002","article-title":"II Consenso Brasileiro de Tuberculose: Diretrizes Brasileiras para Tuberculose","volume":"30","author":"Castelo Filho","year":"2004","journal-title":"J Bras Pneumol"},{"key":"10.1111\/jam.12508-BIB0008|jam12508-cit-0008","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1164\/ajrccm.160.1.9811006","article-title":"Measurement of sputum Mycobacterium tuberculosis mRNA as a surrogate for response to chemotherapy","volume":"160","author":"Desjardin","year":"1999","journal-title":"Am J Respir Crit Care Med"},{"key":"10.1111\/jam.12508-BIB0010|jam12508-cit-0010","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1590\/S1415-790X2005000200006","article-title":"Os fatores associados \u00e0 tuberculose pulmonar e a baciloscopia: uma contribui\u00e7\u00e3o ao diagn\u00f3stico nos servi\u00e7os de sa\u00fade p\u00fablica","volume":"8","author":"Ferreira","year":"2005","journal-title":"Rev Bras Epidemiol"},{"key":"10.1111\/jam.12508-BIB0011|jam12508-cit-0011","volume-title":"Desenvolvimento e Valida\u00e7\u00e3o da Detec\u00e7\u00e3o Molecular da Infec\u00e7\u00e3o por Schistosoma Mansoni em lotes de Moluscos Vetores para Identifica\u00e7\u00e3o de Focos de Transmiss\u00e3o [Dissertation]","author":"Gomes","year":"2008"},{"key":"10.1111\/jam.12508-BIB0012|jam12508-cit-0012","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1128\/JCM.37.2.290-295.1999","article-title":"Quantitative analysis of mRNA as a marker for viability of Mycobacterium tuberculosis","volume":"37","author":"Hellyer","year":"1999a","journal-title":"J Clin Microbiol"},{"key":"10.1111\/jam.12508-BIB0013|jam12508-cit-0013","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1128\/JCM.37.3.518-523.1999","article-title":"Detection of viable Mycobacterium tuberculosis by reverse transcriptase-strand displacement amplification of mRNA","volume":"37","author":"Hellyer","year":"1999b","journal-title":"J Clin Microbiol"},{"key":"10.1111\/jam.12508-BIB0014|jam12508-cit-0014","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1128\/JCM.35.5.1161-1165.1997","article-title":"Single-tube, nested, reverse transcriptase PCR for detection of viable Mycobacterium tuberculosis","volume":"35","author":"Jou","year":"1997","journal-title":"J Clin Microbiol"},{"key":"10.1111\/jam.12508-BIB0015|jam12508-cit-0015","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1128\/JCM.01526-09","article-title":"Sputum Mycobacterium tuberculosis as a marker of bacteriologic clearance in respose to anti-tuberculosis therapy","volume":"48","author":"Li","year":"2010","journal-title":"J Clin Microbiol"},{"key":"10.1111\/jam.12508-BIB0016|jam12508-cit-0016","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1590\/S1806-37132009000700011","article-title":"Performance of nested PCR in the specific detection of Mycobacterium tuberculosis complex in blood samples of pediatric patients","volume":"35","author":"Lima","year":"2009","journal-title":"J Bras Pneumol"},{"key":"10.1111\/jam.12508-BIB0017|jam12508-cit-0017","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.1373\/clinchem.2009.124396","article-title":"Monitoring therapeutic efficacy by real-time detection of Mycobacterium tuberculosis mRNA in sputum","volume":"55","author":"Mdivani","year":"2009","journal-title":"Clin Chem"},{"key":"10.1111\/jam.12508-BIB0018|jam12508-cit-0018","doi-asserted-by":"crossref","first-page":"4143","DOI":"10.1128\/JCM.40.11.4143-4147.2002","article-title":"Rapid and specific detection of Mycobacterium tuberculosis from acid-fast bacillus smear-positive respiratory specimens and BacT\/ALERT MP culture bottles by using fluorogenic probes and real-time PCR","volume":"40","author":"Miller","year":"2002","journal-title":"J Clin Microbiol"},{"key":"10.1111\/jam.12508-BIB0019|jam12508-cit-0019","volume-title":"Secretariat of Health Surveillance in Health. Manual National Laboratory Surveillance of Tuberculosis and other Mycobacteria","author":"Ministry of Health","year":"2008"},{"key":"10.1111\/jam.12508-BIB0020|jam12508-cit-0020","volume-title":"Secretariat of Health Surveillance in Health. Retreatment Cases of Tuberculosis in Brazil","author":"Ministry of Health","year":"2010"},{"key":"10.1111\/jam.12508-BIB0022|jam12508-cit-0022","first-page":"10","article-title":"PCR em tempo real: uma inova\u00e7\u00e3o tecnol\u00f3gica da rea\u00e7\u00e3o em cadeia da polimerase","volume":"33","author":"Novais","year":"2004","journal-title":"Biotecnologia Cienc Desenvolv"},{"key":"10.1111\/jam.12508-BIB0023|jam12508-cit-0023","first-page":"385","article-title":"Applications of real-time PCR technology to mycobacterial research","volume":"124","author":"Parashar","year":"2006","journal-title":"Indian J Med Res"},{"key":"10.1111\/jam.12508-BIB0024|jam12508-cit-0024","first-page":"361","article-title":"Rapid and efficient detection of extrapulmonary Mycobacterium tuberculosis by PCR analysis","volume":"4","author":"Portillo-Gomez","year":"2000","journal-title":"Int J Tuberc Lung Dis"},{"key":"10.1111\/jam.12508-BIB0502|jam12508-cit-0502","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"10.1111\/jam.12508-BIB0027|jam12508-cit-0027","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1590\/S1806-37132008000500010","article-title":"Methodology for characterizing proficiency in interpreting sputum smear microscopy results in the diagnosis of tuberculosis","volume":"34","author":"Vieira","year":"2008","journal-title":"J Bras Pneumol"},{"key":"10.1111\/jam.12508-BIB0028|jam12508-cit-0028","first-page":"287","article-title":"The role of surrogate markers in the clinical evaluation of antituberculous chemotherapy","volume":"4","author":"Wallis","year":"2005","journal-title":"Curr Med Chem"},{"key":"10.1111\/jam.12508-BIB0029|jam12508-cit-0029","volume-title":"Tuberculosis Facts 2006","author":"World Health Organization","year":"2006"},{"key":"10.1111\/jam.12508-BIB0030|jam12508-cit-0030","volume-title":"Global Tuberculosis Control","author":"World Health Organization","year":"2012"}],"container-title":["Journal of Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjam.12508","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjam.12508","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/onlinelibrary.wiley.com\/wol1\/doi\/10.1111\/jam.12508\/fullpdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T09:18:22Z","timestamp":1676366302000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jambio\/article\/117\/1\/266\/6716873"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,29]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,7]]}},"URL":"https:\/\/doi.org\/10.1111\/jam.12508","archive":["Portico"],"relation":{},"ISSN":["1364-5072"],"issn-type":[{"value":"1364-5072","type":"print"}],"subject":[],"published":{"date-parts":[[2014,4,29]]}}}