{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:08:14Z","timestamp":1787015294323,"version":"build-2736575974"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007062","name":"Yangzhou University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31572429"],"award-info":[{"award-number":["31572429"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872988"],"award-info":[{"award-number":["31872988"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To explore the impact of ambient pH on lactate catabolism by Megasphaera elsdenii BE2-2083 and Selenomonas ruminantium HD4 in both pure culture and in binary mixed culture.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>The growth rate, substrate consumption, product formation, enzymatic activity and gene expression of M. elsdenii and S. ruminantium at various pHs were examined. Furthermore, the metabolism of lactate catabolism pathways for M. elsdenii and S. ruminantium in the co-culture system was investigated by chasing the conversion of sodium L-[3-13C]-lactate in nuclear magnetic resonance. In the pure culture systems, ambient pH had significant effects on the growth of M. elsdenii, whereas S. ruminantium was less sensitive to pH changes. In addition, lactate metabolic genes and activities of key enzymes were affected by ambient pH in M. elsdenii and S. ruminantium. In the co-culture system, low ambient pH reduced the contribution lactate catabolism by M. elsdenii.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>M. elsdenii BE2-2083 and S. ruminantium HD4 lactate degradation affected by ambient pH.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Significance and impact of the study<\/jats:title>\n                    <jats:p>This study demonstrates the regulatory mechanisms of lactate decomposing bacteria in lactate catabolism under the condition of subacute ruminal acidosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jam.15464","type":"journal-article","created":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T11:56:22Z","timestamp":1643716582000},"page":"2661-2672","source":"Crossref","is-referenced-by-count":14,"title":["Ambient pH regulates lactate catabolism pathway of the ruminal Megasphaera elsdenii BE2-2083 and Selenomonas ruminantium HD4"],"prefix":"10.1093","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3199-6579","authenticated-orcid":false,"given":"Yaotian","family":"Fan","sequence":"first","affiliation":[{"name":"Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition College of Animal Science and Technology Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangliang","family":"Xia","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition College of Animal Science and Technology Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaqian","family":"Jin","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition College of Animal Science and Technology Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongrong","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition College of Animal Science and Technology Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,4,1]]},"reference":[{"key":"2023011712033080300_jam15464-bib-0001","doi-asserted-by":"crossref","first-page":"2840","DOI":"10.3168\/jds.2010-3783","article-title":"Rumen pH and fermentation characteristics in dairy cows supplemented with Megasphaera elsdenii NCIMB 41125 in early lactation","volume":"94","author":"Aikman","year":"2011","journal-title":"Journal of Dairy Science"},{"key":"2023011712033080300_jam15464-bib-0002","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1111\/jpn.13034","article-title":"Efficacy of Megasphaera elsdenii inoculation in subacute ruminal acidosis in 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