{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T06:56:26Z","timestamp":1792133786319,"version":"4.4.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T00:00:00Z","timestamp":1673913600000},"content-version":"vor","delay-in-days":261,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007687","name":"Svenska L\u00e4kares\u00e4llskapet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007687","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>Spread of carbapenem-resistant Enterobacterales have become a global problem. We characterized extended-spectrum \u03b2-lactamase (ESBL)-producing Enterobacterales from urinary tract infections cases from Allied Hospital Faisalabad, Pakistan.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and Results<\/jats:title>\n                  <jats:p>Eleven (22%, 11\/50) ESBL-producing Enterobacterales (Escherichia coli; n = 10 and Enterobacter hormaechei; n = 1) were recovered and processed through VITEK-2, PCR, rep-PCR followed by whole-genome sequencing (WGS) of ESBL-producing Ent. hormaechei and carbapenem-resistant E. coli isolates. Plasmid transferability of blaNDM-1-producers was assayed by conjugation experiments. All ESBL strains carried the blaCTX-M-15 gene. Of these blaCTX-M-15 producing E. coli, four also carried blaNDM-1 located on transferable plasmids. All E. coli strains belonged to ST448 and displayed similar genetic features including genes for antimicrobial resistance, heavy metal, biocides and virulence. Genomic features of a multidrug-resistant (MDR) Ent. hormaechei were also reported for the first time in Pakistan.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Our findings indicate that blaNDM-1 producing E. coli ST448 is a multidrug, heavy metals and biocides-resistant strain. Therefore, the screening of these isolates may be effective in limiting the MDR bacteria spread in hospitalized patients and within the community.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Significance and Impact of this Study<\/jats:title>\n                  <jats:p>Spread of multi-drug-resistant ESBL-producing bacteria in the clinical settings of Pakistan is a serious challenge and further limiting treatment options in the country. WGS could be used as a tool in the nationwide antibiotic surveillance programme to explore insights of spread and outbreak.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/jam.15482","type":"journal-article","created":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T07:47:43Z","timestamp":1644392863000},"page":"3907-3914","source":"Crossref","is-referenced-by-count":11,"title":["Genomic characterization of high-risk Escherichia coli and Enterobacter hormaechei clones recovered from a single tertiary-care hospital in Pakistan"],"prefix":"10.1093","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5460-5780","authenticated-orcid":false,"given":"Mashkoor","family":"Mohsin","sequence":"first","affiliation":[{"name":"Institute of Microbiology University of Agriculture Faisalabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brekhna","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Medicine Institute of Infection and Immunity, Cardiff University Cardiff UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Usman","family":"Khan","sequence":"additional","affiliation":[{"name":"District Headquarter Hospital Faisalabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arslan","family":"Ali","sequence":"additional","affiliation":[{"name":"Institute of Microbiology University of Agriculture Faisalabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00f6te","family":"Swedberg","sequence":"additional","affiliation":[{"name":"Section for Infectious Diseases, Department of Medical Sciences Uppsala University Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8766-6355","authenticated-orcid":false,"given":"Badrul","family":"Hasan","sequence":"additional","affiliation":[{"name":"Section for Infectious Diseases, Department of Medical Sciences Uppsala University Uppsala Sweden"},{"name":"Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center (BMC) Uppsala University Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,5,1]]},"reference":[{"key":"2023011711214024300_jam15482-bib-0001","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0201613","article-title":"Genomic characterization of NDM-1 and 5, and OXA-181 carbapenemases in uropathogenic Escherichia coli isolates from Riyadh, Saudi Arabia","volume":"13","author":"Abd El Ghany","year":"2018","journal-title":"PLoS One"},{"key":"2023011711214024300_jam15482-bib-0002","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s13756-018-0309-1","article-title":"Prevalence of extended-spectrum-\u03b2-lactamase-producing Enterobacteriaceae: first systematic meta-analysis report from Pakistan","volume":"7","author":"Abrar","year":"2018","journal-title":"Antimicrobial Resistance and Infection Control"},{"key":"2023011711214024300_jam15482-bib-0003","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1080\/19440049.2017.1300686","article-title":"Cadmium, lead, mercury and arsenic in animal feed and feed materials - trend analysis of monitoring results","volume":"34","author":"Adamse","year":"2017","journal-title":"Food Additives & Contaminants. 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