{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T16:30:39Z","timestamp":1777566639039,"version":"3.51.4"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100007421","name":"2020 Li Ka Shing Foundation (LKSF) Cross-Disciplinary Research Grant","doi-asserted-by":"publisher","award":["2020LKSFG07A"],"award-info":[{"award-number":["2020LKSFG07A"]}],"id":[{"id":"10.13039\/100007421","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"publisher","award":["2021A1515110426"],"award-info":[{"award-number":["2021A1515110426"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory","award":["GML2019ZD0606"],"award-info":[{"award-number":["GML2019ZD0606"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["92051118"],"award-info":[{"award-number":["92051118"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070113"],"award-info":[{"award-number":["32070113"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Scientific Research Foundation of Shantou University","award":["NTF21030"],"award-info":[{"award-number":["NTF21030"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>This study examined and characterized the extract for metabolites of Halobacillus marinus HMALI004 to understand their antibacterial activities against opportunistic marine pathogens, that is, Vibrio parahaemolyticus and Vibrio cholerae.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and Results<\/jats:title>\n                  <jats:p>The bacterial strain HMALI004 was characterized as H. marinus, and an antibacterial spectral test revealed its inhibition against two opportunistic marine pathogens (V. parahaemolyticus and V. cholera). Fermentation broth of strain HMALI004 was subjected to column chromatography and high-performance liquid chromatography to separate antibacterial substances. Two compounds were successfully isolated and identified as 1H-pyrrole-2-carboxylic acid and 4-chloro-1H-pyrrole-2-carboxylic acid by mass spectrometry (MS) and nuclear magnetic resonance. The minimal inhibition concentration (MIC) values of 1H-pyrrole-2-carboxylic acid and 4-chloro-1H-pyrrole-2-carboxylic acid for V. parahaemolyticus were 25 \u03bcg\/ml, while their MIC values for V. cholerae were 50 and 100 \u03bcg\/ml, respectively. The reactive oxygen species (ROS) production of two pathogen strains treated with 1H-pyrrole-2-carboxylic acid and 4-chloro-1H-pyrrole-2-carboxylic acid were detected to investigate the antimicrobial mechanism. The results suggested that 4-chloro-1H-pyrrole-2-carboxylic acid exerted enhanced ROS production in V. parahaemolyticus, whereas 1H-pyrrole-2-carboxylic acid had a weaker effect. Both compounds caused a significant rise in ROS production in V. cholerae, causing severe damage to the cell wall and cytoplasm, leading to cell death.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The bacterium H. marinus HMALI004 was isolated from a shrimp pond and was found to produce antimicrobial compounds, which could inhibit the growth of opportunistic marine pathogens V. parahaemolyticus and V. cholerae by increasing ROS.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Significance and Impact of the Study<\/jats:title>\n                  <jats:p>Successfully isolated antibacterial-producing strain, H. marinus HMALI004, and its antimicrobial compounds could be used as biological control agents for marine pathogens.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/jam.15764","type":"journal-article","created":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T07:58:30Z","timestamp":1659686310000},"page":"3030-3040","source":"Crossref","is-referenced-by-count":6,"title":["Identification of antibacterial metabolites produced by a marine bacterium Halobacillus marinus HMALI004"],"prefix":"10.1093","volume":"133","author":[{"given":"Sardar","family":"Ali","sequence":"first","affiliation":[{"name":"Biology Department and Institute of Marine Sciences College of Science, and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7388-8096","authenticated-orcid":false,"given":"Runlin","family":"Cai","sequence":"additional","affiliation":[{"name":"Biology Department and Institute of Marine Sciences College of Science, and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Feng","sequence":"additional","affiliation":[{"name":"Biology Department and Institute of Marine Sciences College of Science, and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianmin","family":"Xie","sequence":"additional","affiliation":[{"name":"Biology Department and Institute of Marine Sciences College of Science, and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yueling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Biology Department and Institute of Marine Sciences College of Science, and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University Shantou China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Biology Department and Institute of Marine Sciences College of Science, and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University Shantou China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,11,1]]},"reference":[{"key":"2023011711242737400_jam15764-bib-0001","doi-asserted-by":"crossref","DOI":"10.1099\/ijsem.0.005047","article-title":"Penaeicolahalotolerans gen. Nov., sp. nov., a novel bacterium of the family Cyclobacteriaceae isolated from a shrimp pond","volume":"71","author":"Ali","year":"2021","journal-title":"International Journal of Systematic and Evolutionary Microbiology"},{"key":"2023011711242737400_jam15764-bib-0002","doi-asserted-by":"crossref","first-page":"2002962","DOI":"10.1002\/adma.202002962","article-title":"Combating implant infections: shifting focus from bacteria to host","volume":"32","author":"Amin Yavari","year":"2020","journal-title":"Advanced Materials"},{"key":"2023011711242737400_jam15764-bib-0003","doi-asserted-by":"crossref","first-page":"4042","DOI":"10.3390\/ijms22084042","article-title":"Prophylaxis and treatment against Klebsiella pneumoniae: current insights on this emerging anti-microbial resistant global threat","volume":"22","author":"Arato","year":"2021","journal-title":"International Journal of Molecular Sciences"},{"key":"2023011711242737400_jam15764-bib-0004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41572-018-0005-8","article-title":"Vibrio spp. infections","volume":"4","author":"Baker-Austin","year":"2018","journal-title":"Nature Reviews. Disease Primers"},{"key":"2023011711242737400_jam15764-bib-0005","doi-asserted-by":"crossref","first-page":"15233","DOI":"10.1039\/C4RA15710A","article-title":"Pyrrole: a resourceful small molecule in key medicinal hetero-aromatics","volume":"5","author":"Bhardwaj","year":"2015","journal-title":"RSC Advances"},{"key":"2023011711242737400_jam15764-bib-0006","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1007\/s00248-013-0190-7","article-title":"Special issue oceans and humans health: the ecology of marine opportunists","volume":"65","author":"Burge","year":"2013","journal-title":"Microbial Ecology"},{"key":"2023011711242737400_jam15764-bib-0007","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1146\/annurev-marine-031920-100429","article-title":"Marine parasites and disease in the era of global climate change","volume":"13","author":"Byers","year":"2021","journal-title":"Annual Review of Marine Science"},{"key":"2023011711242737400_jam15764-bib-0008","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jns.2016.08.007","article-title":"NS1619 regulates the expression of caveolin-1 protein in a time-dependent manner via ROS\/PI3K\/PKB\/FoxO1 signaling pathway in brain tumor microvascular endothelial cells","volume":"369","author":"Cai","year":"2016","journal-title":"Journal of the Neurological Sciences"},{"key":"2023011711242737400_jam15764-bib-0009","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.pt.2019.12.013","article-title":"Modeling pathogen dispersal in marine fish and shellfish","volume":"36","author":"Cantrell","year":"2020","journal-title":"Trends in Parasitology"},{"key":"2023011711242737400_jam15764-bib-0010","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/j.foodcont.2008.08.003","article-title":"Antimicrobial activity and partial characterization of bacteriocin-like inhibitory substances (BLIS) produced by Bifidobacterium infantis BCRC 14602","volume":"20","author":"Cheikhyoussef","year":"2009","journal-title":"Food Control"},{"key":"2023011711242737400_jam15764-bib-0011","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s10482-009-9340-9","article-title":"Halobacillus naozhouensis sp. nov., a moderately halophilic bacterium isolated from a sea anemone","volume":"96","author":"Chen","year":"2009","journal-title":"Antonie Van Leeuwenhoek"},{"key":"2023011711242737400_jam15764-bib-0012","doi-asserted-by":"crossref","DOI":"10.1002\/cbdv.201600446","article-title":"Pyrrole derivatives and diterpene alkaloids from the South China Sea sponge Agelas nakamurai","volume":"14","author":"Chu","year":"2017","journal-title":"Chemistry & Biodiversity"},{"key":"2023011711242737400_jam15764-bib-0013","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1039\/B813236D","article-title":"Biosynthesis of pyrrolylpolyenes in Auxarthron umbrinum","volume":"7","author":"Clark","year":"2009","journal-title":"Organic & Biomolecular Chemistry"},{"key":"2023011711242737400_jam15764-bib-0014","doi-asserted-by":"crossref","first-page":"A83","DOI":"10.1016\/j.vaccine.2019.06.031","article-title":"Antibiotic resistance in Vibrio cholerae: understanding the ecology of resistance genes and mechanisms","volume":"38","author":"Das","year":"2020","journal-title":"Vaccine"},{"key":"2023011711242737400_jam15764-bib-0015","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.3389\/fmicb.2017.01334","article-title":"Isolation and characterization of \u201cTerrein\u201d an antimicrobial and antitumor compound from endophytic fungus aspergillus terreus (JAS\u20132) associated from Achyranthus aspera Varanasi, India","volume":"8","author":"Goutam","year":"2017","journal-title":"Frontiers in Microbiology"},{"key":"2023011711242737400_jam15764-bib-0016","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1111\/jam.14548","article-title":"Identification of antimicrobial metabolites produced by a potential biocontrol actinomycete strain A217","volume":"128","author":"He","year":"2020","journal-title":"Journal of Applied Microbiology"},{"key":"2023011711242737400_jam15764-bib-0017","doi-asserted-by":"crossref","first-page":"313","DOI":"10.3390\/antiox10020313","article-title":"Functions of ROS in macrophages and antimicrobial immunity","volume":"10","author":"Herb","year":"2021","journal-title":"Antioxidants"},{"key":"2023011711242737400_jam15764-bib-0018","doi-asserted-by":"crossref","DOI":"10.1016\/j.phymed.2020.153234","article-title":"Diaporisoindole E inhibits RANKL-induced osteoclastogenesis via suppression of PI3K\/AKT and MAPK signal pathways","volume":"75","author":"Hua","year":"2020","journal-title":"Phytomedicine"},{"key":"2023011711242737400_jam15764-bib-0019","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1021\/acs.jnatprod.5b01091","article-title":"Sclerotiamide: the first non-peptide-based natural product activator of bacterial caseinolytic protease P","volume":"79","author":"Lavey","year":"2016","journal-title":"Journal of Natural Products"},{"key":"2023011711242737400_jam15764-bib-0020","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1007\/s00253-017-8096-9","article-title":"Ecological fitness and virulence features of Vibrio parahaemolyticus in estuarine environments","volume":"101","author":"Lovell","year":"2017","journal-title":"Applied Microbiology and Biotechnology"},{"key":"2023011711242737400_jam15764-bib-0021","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1007\/s00253-011-3567-x","article-title":"Biosynthesis of the nargenicin A1 pyrrole moiety from Nocardia sp. CS682","volume":"93","author":"Maharjan","year":"2012","journal-title":"Applied Microbiology and Biotechnology"},{"key":"2023011711242737400_jam15764-bib-0022","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-63491-w","article-title":"In-vitro analysis of free radical scavenging activities and suppression of LPS-induced ROS production in macrophage cells by Solanum sisymbriifolium extracts","volume":"10","author":"More","year":"2020","journal-title":"Scientific Reports"},{"key":"2023011711242737400_jam15764-bib-0023","doi-asserted-by":"crossref","first-page":"5355","DOI":"10.1007\/s00253-019-09848-2","article-title":"The effect of reactive oxygen species (ROS) and ROS-scavenging enzymes, superoxide dismutase and catalase, on the thermotolerant ability of Corynebacterium glutamicum","volume":"103","author":"Nantapong","year":"2019","journal-title":"Applied Microbiology and Biotechnology"},{"key":"2023011711242737400_jam15764-bib-0024","doi-asserted-by":"crossref","first-page":"1","DOI":"10.20884\/1.oa.2016.12.1.24","article-title":"Vibriosis in aquaculture","volume":"12","author":"Novriadi","year":"2016","journal-title":"Omni-Akuatika"},{"key":"2023011711242737400_jam15764-bib-0025","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s12275-018-7387-x","article-title":"Taxonomic description and genome sequence of Halobacillus marinus sp. nov., a novel strain isolated from Chilika Lake, India","volume":"56","author":"Panda","year":"2018","journal-title":"Journal of Microbiology"},{"key":"2023011711242737400_jam15764-bib-0026","first-page":"659","article-title":"Quorum sensing inhibiting activity of Streptomyces coelicoflavus isolated from soil","volume":"7","author":"Ramadan","year":"2016","journal-title":"Frontiers in Microbiology"},{"key":"2023011711242737400_jam15764-bib-0027","doi-asserted-by":"crossref","first-page":"2019","DOI":"10.1021\/np400119n","article-title":"Antifungal cyclic lipopeptides from Bacillus amyloliquefaciens strain BO5A","volume":"76","author":"Romano","year":"2013","journal-title":"Journal of Natural Products"},{"key":"2023011711242737400_jam15764-bib-0028","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3201\/eid1701.P11101","article-title":"Foodborne illness acquired in the United States\u2014major pathogens","volume":"17","author":"Scallan","year":"2011","journal-title":"Emerging Infectious Diseases"},{"key":"2023011711242737400_jam15764-bib-0029","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1099\/ijs.0.65008-0","article-title":"Halobacillus mangrovei sp. nov., a moderately halophilic bacterium isolated from the black mangrove Avicennia germinans","volume":"58","author":"Soto-Ram\u00edrez","year":"2008","journal-title":"International Journal of Systematic and Evolutionary Microbiology"},{"key":"2023011711242737400_jam15764-bib-0030","first-page":"492","article-title":"Halobacillus gen. Nov., with descriptions of Halobacillus litoralis sp. nov., and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov","volume":"46","author":"Spring","year":"1996","journal-title":"International Journal of Systematic and Evolutionary Microbiology"},{"key":"2023011711242737400_jam15764-bib-0031","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1007\/s00248-017-1051-6","article-title":"Comparative 16SrDNA gene-based microbiota profiles of the Pacific oyster (Crassostrea gigas) and the Mediterranean mussel (Mytilus galloprovincialis) from a shellfish farm (Ligurian Sea, Italy)","volume":"75","author":"Vezzulli","year":"2018","journal-title":"Microbial Ecology"},{"key":"2023011711242737400_jam15764-bib-0032","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1099\/ijs.0.000198","article-title":"Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil","volume":"65","author":"Wang","year":"2015","journal-title":"International Journal of Systematic and Evolutionary Microbiology"},{"key":"2023011711242737400_jam15764-bib-0033","doi-asserted-by":"crossref","first-page":"199","DOI":"10.3390\/md17040199","article-title":"Characterization of antifungal lipopeptide biosurfactants produced by marine bacterium Bacillus sp. CS30","volume":"17","author":"Wu","year":"2019","journal-title":"Marine Drugs"},{"key":"2023011711242737400_jam15764-bib-0034","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1099\/ijs.0.65341-0","article-title":"Halobacillus seohaensis sp. nov., isolated from a marine solar saltern in Korea","volume":"58","author":"Yoon","year":"2008","journal-title":"International Journal of Systematic and Evolutionary Microbiology"},{"key":"2023011711242737400_jam15764-bib-0035","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1021\/acs.iecr.1c04164","article-title":"Purification, fermentation optimization, and antibacterial activity of pyrrole-2-carboxylic acid produced by an endophytic bacterium, Bacillus cereus ZBE, isolated from Zanthoxylum bungeanum","volume":"61","author":"Yue","year":"2022","journal-title":"Industrial & Engineering Chemistry Research"},{"key":"2023011711242737400_jam15764-bib-0036","doi-asserted-by":"crossref","first-page":"3432","DOI":"10.1021\/acs.jnatprod.9b00808","article-title":"Phallusialides A\u2013E, pyrrole-derived alkaloids discovered from a marine-derived Micromonospora sp. bacterium using MS-based metabolomics approaches","volume":"82","author":"Zhang","year":"2019","journal-title":"Journal of Natural Products"},{"key":"2023011711242737400_jam15764-bib-0037","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1007\/s11430-017-9229-x","article-title":"Significance of Vibrio species in the marine organic carbon cycle\u2013a review","volume":"61","author":"Zhang","year":"2018","journal-title":"Science China Earth Sciences"},{"key":"2023011711242737400_jam15764-bib-0038","doi-asserted-by":"crossref","first-page":"5951","DOI":"10.1007\/s00253-017-8396-0","article-title":"Biological activity of lipopeptides from bacillus","volume":"101","author":"Zhao","year":"2017","journal-title":"Applied Microbiology and Biotechnology"},{"key":"2023011711242737400_jam15764-bib-0039","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.fitote.2019.04.011","article-title":"A new phthalazinone derivative and a new isoflavonoid glycoside from lichen-associated Amycolatopsis sp","volume":"135","author":"Zheng","year":"2019","journal-title":"Fitoterapia"}],"container-title":["Journal of Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jam.15764","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jam.15764","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/133\/5\/3030\/48732727\/jambio3030.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/133\/5\/3030\/48732727\/jambio3030.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T15:08:56Z","timestamp":1673968136000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jambio\/article\/133\/5\/3030\/6988972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,1]]},"references-count":39,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,11,1]]},"published-print":{"date-parts":[[2022,11,1]]}},"URL":"https:\/\/doi.org\/10.1111\/jam.15764","relation":{},"ISSN":["1365-2672","1364-5072"],"issn-type":[{"value":"1365-2672","type":"electronic"},{"value":"1364-5072","type":"print"}],"subject":[],"published-other":{"date-parts":[[2022,11,1]]},"published":{"date-parts":[[2022,11,1]]}}}