{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T12:05:05Z","timestamp":1768651505655,"version":"3.49.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Introduced Talents of Shenyang Agricultural University","award":["20153040"],"award-info":[{"award-number":["20153040"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31601586"],"award-info":[{"award-number":["31601586"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Scientific Research Foundation of the Educational Department of Liaoning Province","award":["LSNQN202015"],"award-info":[{"award-number":["LSNQN202015"]}]},{"name":"Starting Grants of Shenyang Agricultural University","award":["880416060"],"award-info":[{"award-number":["880416060"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>Lovastatin has been indicated to impair growth and development of Phytophthora sojae. Therefore, this study was performed to understand the inhibitory mechanism of lovastatin and investigate the metabolic pathway potentially served as a new control target for this plant pathogen.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and Results<\/jats:title>\n                  <jats:p>Whole transcriptome analysis of lovastatin-treated P. sojae was performed by RNA-sequencing. The results revealed that 84 genes were upregulated and 58 were downregulated with more than fourfold changes under treatment. Kyoto Encyclopaedia of Genes and Genomes analysis indicated that the branched-chain amino acids (BCAAs) biosynthesis pathway was abundantly enriched. All enzymes in the BCAAs biosynthesis pathway were identified in the P. sojae genome. Moreover, the study found that the herbicide flumetsulam targeting acetohydroxyacid synthase (AHAS) of the BCAAs biosynthesis pathway could effectively inhibit mycelial growth of P. sojae.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Lovastatin treatment significantly influences the BCAAs biosynthesis pathway in P. sojae. Moreover, the herbicide flumetsulam targets AHAS and inhibits growth of P. sojae.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Significance and Impact of the Study<\/jats:title>\n                  <jats:p>The present study revealed that BCAAs biosynthesis pathway was influenced by lovastatin treatment and its key enzyme AHAS was identified as a potential new control target, which provides clues for exploring more oomycetes to control plant diseases caused by P. sojae.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/jam.15792","type":"journal-article","created":{"date-parts":[[2022,8,24]],"date-time":"2022-08-24T07:05:23Z","timestamp":1661324723000},"page":"3585-3595","source":"Crossref","is-referenced-by-count":4,"title":["Transcriptome reveals BCAAs biosynthesis pathway is influenced by lovastatin and can act as a potential control target in Phytophthora sojae"],"prefix":"10.1093","volume":"133","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8927-0565","authenticated-orcid":false,"given":"Xinyu","family":"Yang","sequence":"first","affiliation":[{"name":"College of Plant Protection Shenyang Agricultural University Shenyang Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qifeng","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Shenyang Agricultural University Shenyang Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jitao","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Shenyang Agricultural University Shenyang Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Plant Protection Shenyang Agricultural University Shenyang Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Shenyang Agricultural University Shenyang Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanhua","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Shenyang Agricultural University Shenyang Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenwu","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Shenyang Agricultural University Shenyang Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,12,1]]},"reference":[{"key":"2023011711281836000_jam15792-bib-0001","doi-asserted-by":"crossref","first-page":"5849","DOI":"10.1021\/acs.biochem.7b00849","article-title":"Bacterial branched-chain amino acid biosynthesis: structures, mechanisms, and drugability","volume":"56","author":"Amorim Franco","year":"2017","journal-title":"Biochemistry"},{"key":"2023011711281836000_jam15792-bib-0002","doi-asserted-by":"crossref","first-page":"R106","DOI":"10.1186\/gb-2010-11-10-r106","article-title":"Differential expression analysis for sequence count data","volume":"11","author":"Anders","year":"2010","journal-title":"Genome Biology"},{"key":"2023011711281836000_jam15792-bib-0003","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s00709-011-0269-2","article-title":"The evolutionary phylogeny of the oomycete \"fungi\"","volume":"249","author":"Beakes","year":"2012","journal-title":"Protoplasma"},{"key":"2023011711281836000_jam15792-bib-0004","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1093\/infdis\/jiw090","article-title":"Statin concentrations below the minimum inhibitory concentration attenuate the virulence of Rhizopus oryzae","volume":"214","author":"Bellanger","year":"2016","journal-title":"The Journal of Infectious Diseases"},{"key":"2023011711281836000_jam15792-bib-0005","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"2023011711281836000_jam15792-bib-0006","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1128\/AAC.50.1.96-103.2006","article-title":"Lovastatin has significant activity against Zygomycetes and interacts synergistically with voriconazole","volume":"50","author":"Chamilos","year":"2006","journal-title":"Antimicrobial Agents and Chemotherapy"},{"key":"2023011711281836000_jam15792-bib-0007","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1094\/PDIS-94-7-0881","article-title":"Distribution, pathotypes, and metalaxyl sensitivity of Phytophthora sojae from Heilongjiang and Fujian provinces in China","volume":"94","author":"Cui","year":"2010","journal-title":"Plant Disease"},{"key":"2023011711281836000_jam15792-bib-0008","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0170873","article-title":"Comparative analysis of sterol acquisition in the oomycetes Saprolegnia parasitica and Phytophthora infestans","volume":"12","author":"Dahlin","year":"2017","journal-title":"PLoS One"},{"key":"2023011711281836000_jam15792-bib-0009","doi-asserted-by":"crossref","first-page":"5463","DOI":"10.1021\/pr200718p","article-title":"Quantitative proteomic analysis revealed lovastatin-induced perturbation of cellular pathways in HL-60 cells","volume":"10","author":"Dong","year":"2011","journal-title":"Journal of Proteome Research"},{"key":"2023011711281836000_jam15792-bib-0010","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1080\/07060661.2018.1445127","article-title":"Management of Phytophthora sojae of soybean: a review and future perspectives","volume":"40","author":"Dorrance","year":"2018","journal-title":"Canadian Journal of Plant Pathology"},{"key":"2023011711281836000_jam15792-bib-0011","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1111\/mpp.12053","article-title":"Acetolactate synthases MoIlv2 and MoIlv6 are required for infection-related morphogenesis in Magnaporthe oryzae","volume":"14","author":"Du","year":"2013","journal-title":"Molecular Plant Pathology"},{"key":"2023011711281836000_jam15792-bib-0012","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1094\/PDIS-03-10-0221","article-title":"Population structure and resistance to mefenoxam of Phytophthora capsici in New York state","volume":"94","author":"Dunn","year":"2010","journal-title":"Plant Disease"},{"key":"2023011711281836000_jam15792-bib-0013","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.7164\/antibiotics.29.1346","article-title":"ML-236A, ML-236B, and ML-236C, new inhibitors of cholesterogenesis produced by Penicillium citrinium","volume":"29","author":"Endo","year":"1976","journal-title":"The Journal of Antibiotics"},{"key":"2023011711281836000_jam15792-bib-0014","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1111\/j.1364-3703.2007.00465.x","article-title":"Phytophthora infestans: the plant (and R gene) destroyer","volume":"9","author":"Fry","year":"2008","journal-title":"Molecular Plant Pathology"},{"key":"2023011711281836000_jam15792-bib-0015","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1094\/PHYTO-01-15-0005-FI","article-title":"Five reasons to consider Phytophthora infestans a reemerging pathogen","volume":"105","author":"Fry","year":"2015","journal-title":"Phytopathology"},{"key":"2023011711281836000_jam15792-bib-0016","first-page":"E9649","article-title":"Commercial AHAS-inhibiting herbicides are promising drug leads for the treatment of human fungal pathogenic infections","volume":"115","author":"Garcia","year":"2018","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"2023011711281836000_jam15792-bib-0017","first-page":"E1091","article-title":"Comprehensive understanding of acetohydroxyacid synthase inhibition by different herbicide families","volume":"114","author":"Garcia","year":"2017","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"2023011711281836000_jam15792-bib-0018","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1111\/j.1742-4658.2012.08505.x","article-title":"Bacterial acetohydroxyacid synthase and its inhibitors--a summary of their structure, biological activity and current status","volume":"279","author":"Gedi","year":"2012","journal-title":"The FEBS Journal"},{"key":"2023011711281836000_jam15792-bib-0019","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1094\/MPMI-19-1295","article-title":"Phytophthora genomics: the plant destroyers' genome decoded","volume":"19","author":"Govers","year":"2006","journal-title":"Molecular Plant-Microbe Interactions"},{"key":"2023011711281836000_jam15792-bib-0020","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1094\/PDIS-02-12-0211-FE","article-title":"Advances in research on phytophthora capsici on vegetable crops in the United States","volume":"96","author":"Granke","year":"2012","journal-title":"Plant Disease"},{"key":"2023011711281836000_jam15792-bib-0021","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1111\/j.1567-1364.2006.00097.x","article-title":"Lovastatin possesses a fungistatic effect against Candida albicans, but does not trigger apoptosis in this opportunistic human pathogen","volume":"6","author":"Gyetvai","year":"2006","journal-title":"FEMS Yeast Research"},{"key":"2023011711281836000_jam15792-bib-0022","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1126\/science.1059344","article-title":"Structural mechanism for statin inhibition of HMG-CoA reductase","volume":"292","author":"Istvan","year":"2001","journal-title":"Science"},{"key":"2023011711281836000_jam15792-bib-0023","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1111\/mpp.12190","article-title":"The top 10 oomycete pathogens in molecular plant pathology","volume":"16","author":"Kamoun","year":"2015","journal-title":"Molecular Plant Pathology"},{"key":"2023011711281836000_jam15792-bib-0024","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: a fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nature Methods"},{"key":"2023011711281836000_jam15792-bib-0025","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1099\/mic.0.034348-0","article-title":"Cytocidal amino acid starvation of Saccharomyces cerevisiae and Candida albicans acetolactate synthase (ilv2\u0394) mutants is influenced by the carbon source and rapamycin","volume":"156","author":"Kingsbury","year":"2010","journal-title":"Microbiology"},{"key":"2023011711281836000_jam15792-bib-0026","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1099\/mic.0.26928-0","article-title":"Cryptococcus neoformans Ilv2p confers resistance to sulfometuron methyl and is required for survival at 37\u00b0C and in vivo","volume":"150","author":"Kingsbury","year":"2004","journal-title":"Microbiology"},{"key":"2023011711281836000_jam15792-bib-0027","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nature Methods"},{"key":"2023011711281836000_jam15792-bib-0028","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1021\/jm301501k","article-title":"Sulfonylureas have antifungal activity and are potent inhibitors of Candida albicans acetohydroxyacid synthase","volume":"56","author":"Lee","year":"2013","journal-title":"Journal of Medicinal Chemistry"},{"key":"2023011711281836000_jam15792-bib-0029","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1186\/1471-2105-12-323","article-title":"RSEM: accurate transcript quantification from RNA-seq data with or without a reference genome","volume":"12","author":"Li","year":"2011","journal-title":"BMC Bioinformatics"},{"key":"2023011711281836000_jam15792-bib-0030","doi-asserted-by":"crossref","first-page":"16315","DOI":"10.1038\/srep16315","article-title":"Acetohydroxyacid synthase FgIlv2 and FgIlv6 are involved in BCAA biosynthesis, mycelial and conidial morphogenesis, and full virulence in Fusarium graminearum","volume":"5","author":"Liu","year":"2015","journal-title":"Scientific Reports"},{"key":"2023011711281836000_jam15792-bib-0031","doi-asserted-by":"crossref","first-page":"1660","DOI":"10.1128\/AAC.34.9.1660","article-title":"Effects of lovastatin (mevinolin) on sterol levels and on activity of azoles in Saccharomyces cerevisiae","volume":"34","author":"Lorenz","year":"1990","journal-title":"Antimicrobial Agents and Chemotherapy"},{"key":"2023011711281836000_jam15792-bib-0032","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1574-6968.2006.00370.x","article-title":"Growth inhibition of Candida species and Aspergillus fumigatus by statins","volume":"262","author":"Macreadie","year":"2006","journal-title":"FEMS Microbiology Letters"},{"key":"2023011711281836000_jam15792-bib-0033","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/s00726-005-0297-3","article-title":"Acetohydroxyacid synthase and its role in the biosynthetic pathway for branched-chain amino acids","volume":"31","author":"McCourt","year":"2006","journal-title":"Amino Acids"},{"key":"2023011711281836000_jam15792-bib-0034","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.abb.2010.09.028","article-title":"Enzymes of the mevalonate pathway of isoprenoid biosynthesis","volume":"505","author":"Miziorko","year":"2011","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"2023011711281836000_jam15792-bib-0035","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1146\/annurev-physiol-020518-114455","article-title":"Branched chain amino acids","volume":"81","author":"Neinast","year":"2019","journal-title":"Annual Review of Physiology"},{"key":"2023011711281836000_jam15792-bib-0036","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1080\/13693780701397673","article-title":"Antifungal activity of statins against Aspergillus species","volume":"45","author":"Qiao","year":"2007","journal-title":"Medical Mycology"},{"key":"2023011711281836000_jam15792-bib-0037","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1111\/mpp.12623","article-title":"Genome-wide characterization of Phytophthora infestans metabolism: a systems biology approach","volume":"19","author":"Rodenburg","year":"2018","journal-title":"Molecular Plant Pathology"},{"key":"2023011711281836000_jam15792-bib-0038","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1186\/s12864-018-4852-1","article-title":"Transcriptome alteration in Phytophthora infestans in response to phenazine-1-carboxylic acid production by Pseudomonas fluorescens strain LBUM223","volume":"19","author":"Roquigny","year":"2018","journal-title":"BMC Genomics"},{"key":"2023011711281836000_jam15792-bib-0039","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1094\/PD-90-1135","article-title":"Diversity of Phytophthora capsici in Northwest Spain: analysis of virulence, metalaxyl response, and molecular characterization","volume":"90","author":"Silvar","year":"2006","journal-title":"Plant Disease"},{"key":"2023011711281836000_jam15792-bib-0040","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1111\/j.1582-4934.2001.tb00172.x","article-title":"Statins: mechanism of action and effects","volume":"5","author":"Stancu","year":"2001","journal-title":"Journal of Cellular and Molecular Medicine"},{"key":"2023011711281836000_jam15792-bib-0041","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1002\/1522-2683(20000601)21:11<2129::AID-ELPS2129>3.0.CO;2-V","article-title":"Proteomics to display lovastatin-induced protein and pathway regulation in rat liver","volume":"21","author":"Steiner","year":"2000","journal-title":"Electrophoresis"},{"key":"2023011711281836000_jam15792-bib-0042","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1364-3703.2006.00373.x","article-title":"Phytophthora sojae: root rot pathogen of soybean and model oomycete","volume":"8","author":"Tyler","year":"2007","journal-title":"Molecular Plant Pathology"},{"key":"2023011711281836000_jam15792-bib-0043","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1126\/science.1128796","article-title":"Phytophthora genome sequences uncover evolutionary origins and mechanisms of pathogenesis","volume":"313","author":"Tyler","year":"2006","journal-title":"Science"},{"key":"2023011711281836000_jam15792-bib-0044","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1111\/j.1567-1364.2006.00194.x","article-title":"Simvastatin reduces ergosterol levels, inhibits growth and causes loss of mtDNA in Candida glabrata","volume":"7","author":"Westermeyer","year":"2007","journal-title":"FEMS Yeast Research"},{"key":"2023011711281836000_jam15792-bib-0045","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.ejmech.2018.11.005","article-title":"Chemical preparation, biological evaluation and 3D-QSAR of ethoxysulfuron derivatives as novel antifungal agents targeting acetohydroxyacid synthase","volume":"162","author":"Wu","year":"2019","journal-title":"European Journal of Medicinal Chemistry"},{"key":"2023011711281836000_jam15792-bib-0046","doi-asserted-by":"crossref","first-page":"9575","DOI":"10.1021\/jacs.1c03988","article-title":"Harzianic acid from Trichoderma afroharzianum is a natural product inhibitor of acetohydroxyacid synthase","volume":"143","author":"Xie","year":"2021","journal-title":"Journal of the American Chemical Society"},{"key":"2023011711281836000_jam15792-bib-0047","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.772994","article-title":"The mevalonate pathway is important for growth, spore production, and the virulence of Phytophthora sojae","volume":"12","author":"Yang","year":"2021","journal-title":"Frontiers in Microbiology"},{"key":"2023011711281836000_jam15792-bib-0048","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1094\/MPMI-05-11-0106","article-title":"Digital gene expression profiling of the Phytophthora sojae transcriptome","volume":"24","author":"Ye","year":"2011","journal-title":"Molecular Plant-Microbe Interactions"},{"key":"2023011711281836000_jam15792-bib-0049","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/omi.2011.0118","article-title":"clusterProfiler: an R package for comparing biological themes among gene clusters","volume":"16","author":"Yu","year":"2012","journal-title":"OMICS"}],"container-title":["Journal of Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jam.15792","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jam.15792","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/133\/6\/3585\/48733541\/jambio3585.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/133\/6\/3585\/48733541\/jambio3585.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T15:29:07Z","timestamp":1673969347000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jambio\/article\/133\/6\/3585\/6989077"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,1]]},"references-count":49,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,12,1]]},"published-print":{"date-parts":[[2022,12,1]]}},"URL":"https:\/\/doi.org\/10.1111\/jam.15792","relation":{},"ISSN":["1365-2672","1364-5072"],"issn-type":[{"value":"1365-2672","type":"electronic"},{"value":"1364-5072","type":"print"}],"subject":[],"published-other":{"date-parts":[[2022,12,1]]},"published":{"date-parts":[[2022,12,1]]}}}