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Our study aimed to identify genetic markers linked to hyperketonemia (HYK) patterns in Holstein cows during early lactation and compare these to HYK\u2010negative cows. We screened 964 cows for HYK using a threshold of BHB \u22651.2\u2009mmol\/L during the first 2\u2009weeks postpartum (screening period, SP). Cows that tested negative initially were retested the following week. Cows were deemed HYK\u2010negative (CON group) if BHB levels were below 1.2\u2009mmol\/L in both tests, while those with BHB levels exceeding this threshold at any test were treated and classified as HYK\u2010positive (HYK+). Post\u2010treatment, HYK+ cows were monitored for two\u2010week follow\u2010up period (FP) and classified based on their recovery: cured (CUR; consistently low BHB), recurrent (REC; fluctuating BHB levels), severe (SEV; high initial BHB that decreased), or chronic (CHR; persistently high BHB). Using 489 cows that were genotyped, a GWAS was conducted using GCTA software, revealing significant associations of several SNPs across different HYK patterns when compared to the CON group. These SNPs were primarily linked to genes affecting milk traits and were enriched in biological pathways relevant to protein glycosylation, inflammatory response, glucose homeostasis, and fatty acid synthesis. Our findings highlight genomic regions, potential candidate genes, and biological pathways related to ketosis, underscoring potential targets for improving health management in dairy cattle. These insights could lead to better strategies for managing ketosis through genetic selection, ultimately enhancing dairy cattle welfare and productivity. Further research with a larger number of cows is recommended to validate these findings and help confirm the implicated SNPs and genes.<\/jats:p>","DOI":"10.1111\/jbg.12875","type":"journal-article","created":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T05:59:36Z","timestamp":1716530376000},"page":"702-721","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Identification of genetic markers associated with hyperketonemia patterns in early lactation Holstein cows"],"prefix":"10.1111","volume":"141","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0975-7926","authenticated-orcid":false,"given":"Maria Malane M.","family":"Muniz","sequence":"first","affiliation":[{"name":"Department of Animal Biosciences, Centre for Genetic Improvement of Livestock University of Guelph  Guelph Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita Couto","family":"Serrenho","sequence":"additional","affiliation":[{"name":"Department of Population Medicine, Ontario Veterinary College University of Guelph  Guelph Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todd","family":"Duffield","sequence":"additional","affiliation":[{"name":"Department of Population Medicine, Ontario Veterinary College University of Guelph  Guelph Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerson A.","family":"de Oliveira Junior","sequence":"additional","affiliation":[{"name":"Department of Animal Biosciences, Centre for Genetic Improvement of Livestock University of Guelph  Guelph Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica A. 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