{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:42:17Z","timestamp":1789486937646,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cardiovasc electrophysiol"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Atrial fibrillation (AF) is one of the most common cardiac arrhythmias. It reduces quality of life and increases the risk of complications such as stroke. Although progress has been made in understanding its pathogenesis, the key cellular subtypes and therapeutic targets remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We applied single\u2010cell transcriptomics to identify critical cellular subtypes in AF. High\u2010dimensional weighted gene co\u2010expression network analysis (hdWGCNA) and machine learning were used to screen AF\u2010related genes. Mendelian randomization (MR) and colocalization analyses were performed to assess causal relationships between these genes and AF.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Single\u2010cell analysis showed a significant increase in macrophages in AF, especially SPP1\u2010expressing macrophages, which may drive AF onset and progression. HdWGCNA identified AF\u2010related gene modules. Three genes, LRCH1, RSRC2 and VAMP2, were found to be causally associated with AF. MR analysis confirmed their significant causal effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The accumulation of SPP1\u2010expressing macrophages may drive the onset and progression of AF. Furthermore, LRCH1, RSRC2, and VAMP2 were identified as key causal genes for AF, providing novel insights into its molecular mechanisms and potential therapeutic targets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jce.70432","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T22:07:33Z","timestamp":1782857253000},"page":"1956-1963","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Cell Transcriptomics and Mendelian Randomization Analysis Reveal Key Genes in Atrial Fibrillation"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4531-332X","authenticated-orcid":false,"given":"Xiangpeng","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery General Hospital of Northern Theater Command Shenyang City China"},{"name":"Graduate Training Base of General Hospital of Northern Theater Command of Dalian Medical University Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5341-9508","authenticated-orcid":false,"given":"Hongquan","family":"Chen","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases (NITFID), Chinese Center for Disease Control and Prevention National Institute for Communicable Disease Control and Prevention Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiguang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery General Hospital of Northern Theater Command Shenyang City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery General Hospital of Northern Theater Command Shenyang City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6523-7488","authenticated-orcid":false,"given":"Huishan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery General Hospital of Northern Theater Command Shenyang City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery General Hospital of Northern Theater Command Shenyang City 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