{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T02:25:09Z","timestamp":1772504709119,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,10]],"date-time":"2025-05-10T00:00:00Z","timestamp":1746835200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Clinical Hypertension"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Given the largely unexplored application of liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS\/MS) steroid analysis in primary aldosteronism (PA), we aimed to investigate its diagnostic utility in PA classification and to characterize steroid secretion patterns across PA subtypes. We retrospectively enrolled 67 patients with PA and collected samples from both peripheral and adrenal veins. We performed a steroid analysis to compare the steroid panel differences between aldosterone\u2010producing adenoma (APA), bilateral adrenal hyperplasia (BAH), and unilateral adrenal hyperplasia (UAH). Analyses included steroid concentrations and secretion ratios, with the latter calculated as individual steroid concentrations divided by total steroid content. The concentrations of 18\u2010hydroxycortisol (18\u2010OHF) were higher in the peripheral veins of patients with APA than in those with BAH and UAH (<jats:italic>p<\/jats:italic> &lt; 0.01). A threshold of 4.83\u00a0ng\/mL for peripheral 18\u2010OHF specifically identified APA cases. In APA cases, adrenal vein secretion ratios of aldosterone, 18\u2010hydroxycorticosterone (18\u2010OHB), and 18\u2010OHF were significantly higher in dominant versus non\u2010dominant adrenal veins (<jats:italic>p<\/jats:italic> &lt; 0.001). A secretion ratio of 18\u2010OHF \u2265 14.6\u2030 and 18\u2010OHB \u2265 4.03\u2030 from the adrenal vein achieved 100% specificity for identifying the dominant secretory side in cases of APA. Collectively, our findings demonstrate that LC\u2010MS\/MS steroid profiling effectively differentiates APA from other PA subtypes. The biochemical criteria for the secretion ratios of 18\u2010OHF and 18\u2010OHB from the adrenal vein\u00a0provide objective criteria for lateralization diagnosis in APA. These findings could refine diagnostic strategies for PA subtyping.<\/jats:p>","DOI":"10.1111\/jch.70062","type":"journal-article","created":{"date-parts":[[2025,5,10]],"date-time":"2025-05-10T07:47:58Z","timestamp":1746863278000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["LC\u2010MS\/MS\u2010Based Assay for Steroid Profiling in Peripheral and Adrenal Venous Samples for the Subtyping of Primary Aldosteronism"],"prefix":"10.1111","volume":"27","author":[{"given":"Xiuqing","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Endocrinology the First Affiliated Hospital Fujian Medical University  Fuzhou China"},{"name":"Department of Endocrinology National Regional Medical Center Binhai Campus of the First Affiliated Hospital Fujian Medical University  Fuzhou China"},{"name":"Clinical Research Center for Metabolic Diseases of Fujian Province the First Affiliated Hospital Fujian Medical University  Fuzhou China"},{"name":"Fujian Key Laboratory of Glycolipid and Bone Mineral Metabolism the First Affiliated Hospital Fujian Medical University  Fuzhou China"},{"name":"Diabetes Research Institute of Fujian Province the First Affiliated Hospital Fujian Medical University  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinyi","family":"Li","sequence":"additional","affiliation":[{"name":"Fujian Medical University  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linjing","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology the First Affiliated Hospital Fujian Medical University  Fuzhou China"},{"name":"Department of Endocrinology National Regional Medical Center Binhai Campus of the First Affiliated Hospital Fujian Medical University  Fuzhou China"},{"name":"Clinical Research Center for Metabolic Diseases of Fujian Province the First Affiliated Hospital Fujian Medical 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