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Proteolytic enzymes such as matrix metalloproteinase (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>) and closely related a disintegrin and metalloproteinase with thrombospondin motifs (<jats:styled-content style=\"fixed-case\">ADAMTS<\/jats:styled-content>) have been assigned key roles in the highly regulated cyclic remodelling of the endometrial <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>. We have previously shown that <jats:styled-content style=\"fixed-case\">ADAMTS<\/jats:styled-content>\u20101 undergoes spatiotemporal changes in human endometrial stromal cells under the regulation of gonadal steroids. This suggests that other <jats:styled-content style=\"fixed-case\">ADAMTS<\/jats:styled-content> subtypes, known as aggrecanases, may contribute to the <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content> remodelling events that occur in female physiological cycles and in preparation for pregnancy. To determine whether progesterone (P4), 17\u03b2\u2010estradiol (E2), or dihydrotestosterone (<jats:styled-content style=\"fixed-case\">DHT<\/jats:styled-content>), alone or in combination, are capable of regulating <jats:styled-content style=\"fixed-case\">ADAMTS<\/jats:styled-content>\u20104, \u20105, \u20108 or \u20109 expression in human endometrial stromal cells <jats:italic>in vitro<\/jats:italic>. Real\u2010time quantitative <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and Western blot analysis were used to measure <jats:styled-content style=\"fixed-case\">ADAMTSs mRNA<\/jats:styled-content> and protein levels in primary cultures of human endometrial stromal cells (<jats:italic>n<\/jats:italic>\u00a0=\u00a012). P4, <jats:styled-content style=\"fixed-case\">DHT<\/jats:styled-content> but not E2 have regulatory effects on <jats:styled-content style=\"fixed-case\">ADAMTS<\/jats:styled-content>\u20108, \u20109 and \u20105 expression. Combined treatment with gonadal steroids did not show any synergistic or antagonistic effects. However, the synthetic steroid antagonists RU486 and hydroxyflutamide specifically inhibited the P4\u2010 or <jats:styled-content style=\"fixed-case\">DHT<\/jats:styled-content>\u2010mediated regulatory effects on <jats:styled-content style=\"fixed-case\">ADAMTS<\/jats:styled-content> expression. These studies provide evidence that the regulation of aggrecanases by gonadal steroids in human endometrial stromal cells may play an important role during decidualization.<\/jats:p>","DOI":"10.1111\/jcmm.12110","type":"journal-article","created":{"date-parts":[[2013,8,16]],"date-time":"2013-08-16T03:29:30Z","timestamp":1376623770000},"page":"1325-1334","source":"Crossref","is-referenced-by-count":16,"title":["Gonadal steroids regulate the expression of aggrecanases in human endometrial stromal cells <i>in vitro<\/i>"],"prefix":"10.1111","volume":"17","author":[{"given":"Jiadi","family":"Wen","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology University of British Columbia Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University of British Columbia Vancouver BC 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