{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:05:24Z","timestamp":1791198324096,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,8,15]],"date-time":"2013-08-15T00:00:00Z","timestamp":1376524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The enhancement of endogenous angiogenesis after stroke will be critical in neurorepair therapies where endothelial progenitor cells (<jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content>s) might be key players. Our aim was to determine the influence of cerebral ischaemia and the role of matrix metalloproteinase\u20109 (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109) on the angiogenic function of <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content>s. Permanent focal cerebral ischaemia was induced by middle cerebral artery (<jats:styled-content style=\"fixed-case\">MCA<\/jats:styled-content>) occlusion in <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109\/knockout (MMP\u20109\/KO) and wild\u2010type (<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>) mice. <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content>s were obtained for cell counting after ischaemia (6 and 24\u00a0hrs) and in control animals. Matrigel<jats:sup>\u2122<\/jats:sup> assays and time\u2010lapse imaging were conducted to monitor angiogenic function of <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>9\u2010deficient <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content>s or after treatment with <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 inhibitors. Focal cerebral ischaemia increased the number of early <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content>s, while <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 deficiency decreased their number in non\u2010ischaemic mice and delayed their release after ischaemia. Late outgrowth endothelial cells (<jats:styled-content style=\"fixed-case\">OEC<\/jats:styled-content>s) from ischaemic mice shaped more vessel structures than controls, while <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 deficiency reduced the angiogenic abilities of <jats:styled-content style=\"fixed-case\">OEC<\/jats:styled-content>s to form vascular networks, <jats:italic>in vitro<\/jats:italic>. Treatment with the <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content> inhibitor GM6001 and the specific <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 inhibitor I also decreased the number of vessel structures shaped by both human and mouse <jats:styled-content style=\"fixed-case\">WT OEC<\/jats:styled-content>s, while exogenous <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 could not revert the impaired angiogenic function in <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109\/<jats:styled-content style=\"fixed-case\">KO OEC<\/jats:styled-content>s. Finally, time\u2010lapse imaging showed that the extension of vascular networks was influenced by cerebral ischaemia and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 deficiency early during the vascular network formation followed by a dynamic vessel remodelling. We conclude that focal cerebral ischaemia triggers the angiogenic responses of <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content>s, while <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 plays a key role in the formation of vascular networks by <jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content>s.<\/jats:p>","DOI":"10.1111\/jcmm.12116","type":"journal-article","created":{"date-parts":[[2013,8,15]],"date-time":"2013-08-15T14:33:34Z","timestamp":1376577214000},"page":"1543-1553","source":"Crossref","is-referenced-by-count":33,"title":["Cerebral ischaemia and matrix metalloproteinase\u20109 modulate the angiogenic function of early and late outgrowth endothelial progenitor cells"],"prefix":"10.1111","volume":"17","author":[{"given":"Anna","family":"Morancho","sequence":"first","affiliation":[{"name":"Neurovascular Research Laboratory Vall d'Hebron Research Institute Universitat Aut\u00f2noma de Barcelona Barcelona 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