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Although animal models and human trials have shown mechanisms of motorprotein proteolysis, not much is known about intrinsic changes of muscle functionality in cancer patients suffering from muscle cachexia, and deeper insights into cachexia pathology in humans are needed. To address this question, <jats:italic>rectus abdominis<\/jats:italic> muscle samples were collected from several surgical control, non\u2010cachectic and cachectic cancer patients and processed for skinned fibre biomechanics, molecular <jats:italic>in vitro<\/jats:italic> motility assays, myosin isoform protein compositions and quantitative ubiquitin polymer protein analysis. In pre\u2010cachectic and cachectic cancer patient samples, maximum force was significantly compromised compared with controls, but showed an unexpected increase in myofibrillar <jats:styled-content style=\"fixed-case\"><jats:roman>Ca<\/jats:roman><\/jats:styled-content><jats:sup>2+<\/jats:sup> sensitivity consistent with a shift from slow to fast myosin isoform expression seen in <jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content> analysis and <jats:italic>in vitro<\/jats:italic> motility assays. Force deficit was specific for \u2018cancer\u2019, but not linked to presence of cachexia. Interestingly, quantitative ubiquitin immunoassays revealed no major changes in static ubiquitin polymer protein profiles, whether cachexia was present or not and were shown to mirror profiles in control patients. Our study on muscle function in cachectic patients shows that abdominal wall skeletal muscle in cancer cachexia shows signs of weakness that can be partially attributed to intrinsic changes to contractile motorprotein function. On protein levels, static ubiquitin polymeric distributions were unaltered, pointing towards evenly up\u2010regulated ubiquitin protein turnover with respect to ubiquitin conjugation, proteasome degradation and de\u2010ubiquitination.<\/jats:p>","DOI":"10.1111\/jcmm.12165","type":"journal-article","created":{"date-parts":[[2013,11,20]],"date-time":"2013-11-20T04:39:45Z","timestamp":1384922385000},"page":"69-79","source":"Crossref","is-referenced-by-count":21,"title":["Motor protein function in skeletal abdominal muscle of cachectic cancer patients"],"prefix":"10.1111","volume":"18","author":[{"given":"Sultan","family":"Taskin","sequence":"first","affiliation":[{"name":"Institute of Physiology and Pathophysiology Ruprecht\u2010Karls\u2010University Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera Isabell","family":"Stumpf","sequence":"additional","affiliation":[{"name":"Institute of Physiology and Pathophysiology 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