{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T05:00:07Z","timestamp":1771650007486,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,1,3]],"date-time":"2014-01-03T00:00:00Z","timestamp":1388707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Clinically reported reparative benefits of mesenchymal stromal cells (<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s) are majorly attributed to strong immune\u2010modulatory abilities not exactly shared by fibroblasts. However, <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s remain heterogeneous populations, with unique tissue\u2010specific subsets, and lack of clear\u2010cut assays defining therapeutic stromal subsets adds further ambiguity to the field. In this context, in\u2010depth evaluation of cellular characteristics of <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s from proximal oro\u2010facial tissues: dental pulp (<jats:styled-content style=\"fixed-case\">DPSC<\/jats:styled-content>s) and periodontal ligament (<jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s) from identical donors provides an opportunity to evaluate exclusive niche\u2010specific influences on multipotency and immune\u2010modulation. Exhaustive cell surface profiling of <jats:styled-content style=\"fixed-case\">DPSC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s indicated key differences in expression of mesenchymal (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>105) and pluripotent\/multipotent stem cell\u2013associated cell surface antigens: <jats:styled-content style=\"fixed-case\">SSEA<\/jats:styled-content>4, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>117, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>123 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>29. <jats:styled-content style=\"fixed-case\">DPSC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s exhibited strong chondrogenic potential, but only <jats:styled-content style=\"fixed-case\">DPSC<\/jats:styled-content>s exhibited adipogenic and osteogenic propensities. <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s expressed immuno\u2010stimulatory\/immune\u2010adhesive ligands like <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>50, upon priming with <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u03b3, unlike <jats:styled-content style=\"fixed-case\">DPSC<\/jats:styled-content>s, indicating differential response patterns to pro\u2010inflammatory cytokines. Both <jats:styled-content style=\"fixed-case\">DPSC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s were hypo\u2010immunogenic and did not elicit robust allogeneic responses despite exposure to <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u03b3 or <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u03b1. Interestingly, only <jats:styled-content style=\"fixed-case\">DPSC<\/jats:styled-content>s attenuated mitogen\u2010induced lympho\u2010proliferative responses and priming with either <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u03b3 or <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u03b1 enhanced immuno\u2010modulation capacity. In contrast, primed or unprimed <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s lacked the ability to suppress polyclonal T cell blast responses. This study indicates that stromal cells from even topographically related tissues do not necessarily share identical <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content> properties and emphasizes the need for a thorough functional testing of <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s from diverse sources with respect to multipotency, immune parameters and response to pro\u2010inflammatory cytokines before translational usage.<\/jats:p>","DOI":"10.1111\/jcmm.12192","type":"journal-article","created":{"date-parts":[[2014,1,3]],"date-time":"2014-01-03T10:09:23Z","timestamp":1388743763000},"page":"344-354","source":"Crossref","is-referenced-by-count":52,"title":["Functional differences in mesenchymal stromal cells from human dental pulp and periodontal ligament"],"prefix":"10.1111","volume":"18","author":[{"given":"Anoop Babu","family":"Vasandan","sequence":"first","affiliation":[{"name":"School of Regenerative Medicine (SORM) Manipal University Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shilpa Rani","family":"Shankar","sequence":"additional","affiliation":[{"name":"School of Regenerative Medicine (SORM) Manipal University Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priya","family":"Prasad","sequence":"additional","affiliation":[{"name":"School of Regenerative Medicine (SORM) Manipal University Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vulugundam","family":"Sowmya Jahnavi","sequence":"additional","affiliation":[{"name":"School of Regenerative Medicine (SORM) Manipal University Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramesh Ramachandra","family":"Bhonde","sequence":"additional","affiliation":[{"name":"School of Regenerative Medicine (SORM) Manipal University Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susarla","family":"Jyothi Prasanna","sequence":"additional","affiliation":[{"name":"School of Regenerative Medicine (SORM) Manipal University Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240600855905"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2004-0308"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2009.0485"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2011.0722"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-012-9365-8"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509340867"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4317\/medoral.17925"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.459"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2012.56"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.11.014"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009016"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368912X663533"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e31827a93dd"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-01-0433"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874325001105010253"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e31829853ce"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1559"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2006.03.019"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.03.007"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.10.3838"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2004.12.013"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2010.090729"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16627-0"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.12.2813"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.11.021"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-013-1630-x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0937635100"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2012.0430"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/SCS.0b013e31816aabc8"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200738129"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006498"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/2045-824X-3-5"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21704"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0491"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.12192","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.12192","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.12192","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.12192","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T10:26:11Z","timestamp":1696155971000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.12192"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,3]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1111\/jcmm.12192"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.12192","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,3]]}}}