{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T18:24:45Z","timestamp":1770834285739,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,1,20]],"date-time":"2014-01-20T00:00:00Z","timestamp":1390176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Activation of poly(<jats:styled-content style=\"fixed-case\">ADP<\/jats:styled-content>\u2010ribose) polymerases (<jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>s) is considered a key event in the molecular and cellular processes leading from acute asthma attacks to bronchial hyper\u2010reactivity, leucocyte recruitment, chronic inflammation, airway remodelling and lung damage. The present investigation has been carried out to investigate the action of hydroxyl\u2010dimethylaminomethyl\u2010thieno[2,3\u2010c]isoquinolin\u20105(4<jats:italic>H<\/jats:italic>)\u2010one (<jats:styled-content style=\"fixed-case\">HYDAMTIQ<\/jats:styled-content>), a new potent <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content> inhibitor, in the process leading from asthma\u2010like events to airway damage. Ovalbumin\u2010sensitized guinea pigs exposed two times to allergen inhalation were treated for 8\u00a0days with vehicle or <jats:styled-content style=\"fixed-case\">HYDAMTIQ<\/jats:styled-content>. Asthma\u2010like signs, bronchial hyper\u2010reactivity to methacholine, cytokine production, histamine release from mast cells, airway remodelling, collagen deposition and lung damage were evaluated. Repeated <jats:styled-content style=\"fixed-case\">HYDAMTIQ<\/jats:styled-content> administration (1\u201010\u00a0mg\/kg\/day i.p.) reduced lung <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content> activity, delayed the appearance and reduced the severity of allergen\u2010induced cough and dyspnoea and dampened the increased bronchial responses to methacholine. <jats:styled-content style=\"fixed-case\">HYDAMTIQ<\/jats:styled-content>\u2010treated animals presented reduced bronchial or alveolar abnormalities, lower number of eosinophils and other leucocytes in the lung and decreased smooth muscle or goblet cell hyperplasia. The treatment also reduced lung oxidative stress markers, such as malondialdehyde or 8\u2010hydroxy\u20102\u2032\u2010deoxyguanosine and the lung content of pro\u2010inflammatory cytokines (<jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1, interleukin (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>)\u20101\u03b2, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20105, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201018). Finally, mast cells isolated from the peritoneal or pleural cavities of sensitized, <jats:styled-content style=\"fixed-case\">HYDAMTIQ<\/jats:styled-content>\u2010treated animals had a reduced ability to release histamine when exposed to ovalbumin <jats:italic>in vitro<\/jats:italic>. Our findings support the proposal that <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content> inhibitors could have a therapeutic potential to reduce chronic lung inflammation, airway damage and remodelling in severe unresponsive asthmatic patients.<\/jats:p>","DOI":"10.1111\/jcmm.12197","type":"journal-article","created":{"date-parts":[[2014,1,21]],"date-time":"2014-01-21T03:02:53Z","timestamp":1390273373000},"page":"468-479","source":"Crossref","is-referenced-by-count":29,"title":["Poly(<scp>ADP<\/scp>\u2010ribose) polymerase inhibition with <scp>HYDAMTIQ<\/scp> reduces allergen\u2010induced asthma\u2010like reaction, bronchial hyper\u2010reactivity and airway remodelling"],"prefix":"10.1111","volume":"18","author":[{"given":"Laura","family":"Lucarini","sequence":"first","affiliation":[{"name":"Department of NEUROFARBA Section of Pharmacology and Toxicology University of Florence Florence 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