{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T10:40:41Z","timestamp":1791974441593,"version":"4.4.0"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,12,25]],"date-time":"2013-12-25T00:00:00Z","timestamp":1387929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mechanisms of latent tuberculosis (<jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>) infection remain elusive. Roles of micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>) have been highlighted in pathogen\u2013host interactions recently. To identify mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s involved in the immune response to <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>, expression profiles of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells from patients with latent <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>, active <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> and healthy controls were investigated by microarray assay and validated by RT\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. Gene ontology (<jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content>) and Kyoto Encyclopedia of Genes and Genomes (<jats:styled-content style=\"fixed-case\">KEGG<\/jats:styled-content>) pathway analysis were used to analyse the significant functions and involvement in signalling pathways of the differentially expressed mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s. To identify potential target genes for miR\u201029, interferon\u2010\u03b3 (<jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3) m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression was measured by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Our results showed that 27 mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were deregulated among the three groups. <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> results were generally consistent with the microarray data. We observed an inverse correlation between miR\u201029 level and <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression in <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells. <jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">KEGG<\/jats:styled-content> pathway analysis showed that the possible target genes of deregulated mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were significantly enriched in mitogen\u2010activated protein kinase signalling pathway, focal adhesion and <jats:styled-content style=\"fixed-case\">extracellular matrix<\/jats:styled-content> receptor interaction, which might be involved in the transition from latent to active <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>. In all, for the first time, our study revealed that some mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells were altered in latent and active <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>. Function and pathway analysis highlighted the possible involvement of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010deregulated m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>. The study might help to improve understanding of the relationship between mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells and <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>, and laid an important foundation for further identification of the underlying mechanisms of latent <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> infection and its reactivation.<\/jats:p>","DOI":"10.1111\/jcmm.12205","type":"journal-article","created":{"date-parts":[[2013,12,26]],"date-time":"2013-12-26T01:04:53Z","timestamp":1388019893000},"page":"503-513","source":"Crossref","is-referenced-by-count":37,"title":["Deregulated micro<scp>RNA<\/scp>s in <scp>CD<\/scp>4<sup>+<\/sup> T cells from individuals with latent tuberculosis <i>versus<\/i> active tuberculosis"],"prefix":"10.1111","volume":"18","author":[{"given":"Yurong","family":"Fu","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Weifang Medical University Weifang China"},{"name":"Department of Laboratory Medicine of Affiliated Hospital of Weifang Medical University Key Laboratory of Clinical Laboratory Diagnostics in Universities of Shandong Weifang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengjun","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine of Affiliated Hospital of Weifang Medical University Key Laboratory of Clinical Laboratory Diagnostics in Universities of Shandong Weifang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine of Affiliated Hospital of Weifang Medical University Key Laboratory of Clinical Laboratory Diagnostics in Universities of Shandong Weifang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruifang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Weifang Medical University Weifang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,12,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903856"},{"key":"e_1_2_7_3_1","volume-title":"Global tuberculosis control: surveillance, planning and financing","author":"World Health Organization","year":"2009"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.33073\/pjm-2012-001","article-title":"Latent M.\u00a0tuberculosis infection\u2010pathogenesis, diagnosis, treatment and prevention strategies","volume":"61","author":"Druszczy\u0144ska M","year":"2012","journal-title":"Pol J 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