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Prior investigations have proposed that integrins play a central role in <jats:styled-content style=\"fixed-case\">PCO<\/jats:styled-content> and we found that, in a mouse fibre cell removal model of cataract surgery, expression of \u03b1<jats:sub>V<\/jats:sub> integrin and its interacting \u03b2\u2010subunits \u03b21, \u03b25, \u03b26, \u03b28 are up\u2010regulated concomitant with \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>s following surgery. To test the hypothesis that \u03b1<jats:sub>V<\/jats:sub> integrins are functionally important in <jats:styled-content style=\"fixed-case\">PCO<\/jats:styled-content> pathogenesis, we created mice lacking the \u03b1<jats:sub>V<\/jats:sub> integrin subunit in all lens cells. Adult lenses lacking \u03b1<jats:sub>V<\/jats:sub> integrins are transparent and show no apparent morphological abnormalities when compared with control lenses. However, following surgical fibre cell removal, the <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>s in control eyes increased cell proliferation, and up\u2010regulated the expression of \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>, \u03b21\u2010integrin, fibronectin, tenascin\u2010C and transforming growth factor beta (<jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2)\u2013induced protein within 48\u00a0hrs, while <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>s lacking \u03b1<jats:sub>V<\/jats:sub> integrins exhibited much less cell proliferation and little to no up\u2010regulation of any of the fibrotic markers tested. This effect appears to result from the known roles of \u03b1<jats:sub>V<\/jats:sub> integrins in latent <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 activation as \u03b1<jats:sub>V<\/jats:sub> integrin null lenses do not exhibit detectable <jats:styled-content style=\"fixed-case\">SMAD<\/jats:styled-content>\u20103 phosphorylation after surgery, while this occurs robustly in control lenses, consistent with the known roles for <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 in fibrotic <jats:styled-content style=\"fixed-case\">PCO<\/jats:styled-content>. These data suggest that therapeutics antagonizing \u03b1<jats:sub>V<\/jats:sub> integrin function could be used to prevent fibrotic <jats:styled-content style=\"fixed-case\">PCO<\/jats:styled-content> following cataract surgery.<\/jats:p>","DOI":"10.1111\/jcmm.12213","type":"journal-article","created":{"date-parts":[[2014,2,4]],"date-time":"2014-02-04T22:19:18Z","timestamp":1391552358000},"page":"656-670","source":"Crossref","is-referenced-by-count":81,"title":["The roles of \u03b1<sub>V<\/sub> integrins in lens <scp>EMT<\/scp> and posterior capsular opacification"],"prefix":"10.1111","volume":"18","author":[{"given":"Fahmy A.","family":"Mamuya","sequence":"first","affiliation":[{"name":"Department of Biological Sciences University of Delaware Newark DE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Delaware Newark DE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria H.","family":"Roop","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Delaware Newark DE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Scheiblin","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Delaware Newark DE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jocelyn C.","family":"Zajac","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Delaware Newark DE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melinda K.","family":"Duncan","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Delaware Newark DE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)70803-5"},{"key":"e_1_2_9_3_1","article-title":"Global initiative for the elimination of blindness","author":"World Health Organization","year":"2008","journal-title":"WHO\/PBL"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1993.01090080037016"},{"key":"e_1_2_9_5_1","first-page":"43","article-title":"actuality in cataract treatment","volume":"63","author":"Laroche L","year":"2013","journal-title":"Rev Prat"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.85.6.635"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2011.10.001"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000084508"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2008.10.016"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2009.06.031"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/archophthalmol.2009.3"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.icu.0000193074.24746.e6"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713689409015091"},{"key":"e_1_2_9_14_1","first-page":"2201","article-title":"Identification of transforming growth factor\u2010beta as an immunosuppressive factor in aqueous humor","volume":"32","author":"Cousins SW","year":"1991","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90115-S"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00971-6"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.04.004"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2008.06.020"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2004.05.058"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002010409"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/02713680490513137"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/07357900701522638"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01551"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03-0856"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.12.5860"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0947"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(01)00645-1"},{"key":"e_1_2_9_28_1","doi-asserted-by":"crossref","unstructured":"ScheiblinaDA GaobJ CaplanaJL et\u00a0al.Beta\u20101 integrin is important for the structural maintenance and homeostasis of differentiating fiber cells 2014. 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