{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T09:37:38Z","timestamp":1790329058520,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,6,3]],"date-time":"2014-06-03T00:00:00Z","timestamp":1401753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Octamer binding trascription factor 4 (Oct4) is a transcription factor of <jats:styled-content style=\"fixed-case\">POU<\/jats:styled-content> family specifically expressed in embryonic stem cells (<jats:styled-content style=\"fixed-case\">ESC<\/jats:styled-content>s). A role for maintaining pluripotency and self\u2010renewal of <jats:styled-content style=\"fixed-case\">ESC<\/jats:styled-content>s is assigned to Oct4 as a pluripotency marker. Oct4 can also be detected in adult stem cells such as bone marrow\u2010derived mesenchymal stem cells. Several studies suggest a role for Oct4 in sustaining self\u2010renewal capacity of adult stem cells. However, Oct4 gene ablation in adult stem cells revealed no abnormalities in tissue turnover or regenerative capacity. In the present study we have conspicuously found pulmonary Oct4\u2010positive cells closely resembling the morphology of telocytes (<jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content>s). These cells were found in the perivascular and peribronchial areas and their presence and location were confirmed by electron microscopy. Moreover, we have used Oct4\u2010<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content> transgenic mice which revealed a similar localization of the Oct4\u2010<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content> signal. We also found that Oct4 co\u2010localized with several described <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content> markers such as vimentin, Sca\u20101, platelet\u2010derived growth factor receptor\u2010beta C\u2010kit and <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>. By flow cytometry analyses carried out with Oct4\u2010<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content> reporter mice, we described a population of Ep<jats:styled-content style=\"fixed-case\">CAM<\/jats:styled-content><jats:sup>neg<\/jats:sup>\/<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45<jats:sup>neg<\/jats:sup>\/Oct4\u2010<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content><jats:sup>pos<\/jats:sup> that in culture displayed <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content> features. These results were supported by q<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> with m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> isolated from lungs by using laser capture microdissection. In addition, Oct4\u2010positive cells were found to express Nanog and Klf4 m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>. It is concluded for the first time that <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content>s in adult lung mouse tissue comprise Oct4\u2010positive cells, which express pluripotency\u2010related genes and represent therefore a population of adult stem cells which might contribute to lung regeneration.<\/jats:p>","DOI":"10.1111\/jcmm.12295","type":"journal-article","created":{"date-parts":[[2014,6,3]],"date-time":"2014-06-03T06:35:48Z","timestamp":1401777348000},"page":"1321-1333","source":"Crossref","is-referenced-by-count":35,"title":["Phenotypical and ultrastructural features of Oct4\u2010positive cells in the adult mouse lung"],"prefix":"10.1111","volume":"18","author":[{"given":"Celimene","family":"Galiger","sequence":"first","affiliation":[{"name":"Department of Lung Development and Remodeling Max\u2010Planck\u2010Institute for Heart and Lung Research Bad Nauheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sawa","family":"Kostin","sequence":"additional","affiliation":[{"name":"Core Lab for Molecular and Structural Biology Max\u2010Planck\u2010Institute for Heart and Lung Research Bad Nauheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Golec","sequence":"additional","affiliation":[{"name":"Department of Lung Development and Remodeling Max\u2010Planck\u2010Institute for Heart and Lung Research Bad Nauheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Ahlbrecht","sequence":"additional","affiliation":[{"name":"Department of Lung Development and Remodeling Max\u2010Planck\u2010Institute for Heart and Lung Research Bad Nauheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven","family":"Becker","sequence":"additional","affiliation":[{"name":"Department of Lung Development and Remodeling Max\u2010Planck\u2010Institute for Heart and Lung Research Bad Nauheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihaela","family":"Gherghiceanu","sequence":"additional","affiliation":[{"name":"\u201cVictor Babes\u201d National Institute for Pathology Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurentiu M.","family":"Popescu","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Medicine \u201cCarol Davila\u201d University of Medicine and Pharmacy Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rory E.","family":"Morty","sequence":"additional","affiliation":[{"name":"Department of Lung Development and Remodeling Max\u2010Planck\u2010Institute for Heart and Lung Research Bad Nauheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Seeger","sequence":"additional","affiliation":[{"name":"Department of Lung 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