{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:58:30Z","timestamp":1791907110568,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,6,9]],"date-time":"2014-06-09T00:00:00Z","timestamp":1402272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In eukaryotic cells, mitochondrial dysfunction is associated with a variety of human diseases. Delivery of exogenous functional mitochondria into damaged cells has been proposed as a mechanism of cell transplant and physiological repair for damaged tissue. We here demonstrated that isolated mitochondria can be transferred into homogeneic and xenogeneic cells by simple co\u2010incubation using genetically labelled mitochondria, and elucidated the mechanism and the effect of direct mitochondrial transfer. Intracellular localization of exogenous mitochondria was confirmed by\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    , real\u2010time\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    , live fluorescence imaging, three\u2010dimensional reconstruction imaging, continuous time\u2010lapse microscopic observation, flow cytometric analysis and immunoelectron microscopy. Isolated homogeneic mitochondria were transferred into human uterine endometrial gland\u2010derived mesenchymal cells in a dose\u2010dependent manner. Moreover, mitochondrial transfer rescued the mitochondrial respiratory function and improved the cellular viability in mitochondrial\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    \u2010depleted cells and these effects lasted several days. Finally, we discovered that mitochondrial internalization involves macropinocytosis. In conclusion, these data support direct transfer of exogenous mitochondria as a promising approach for the treatment of various diseases.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.12316","type":"journal-article","created":{"date-parts":[[2014,6,9]],"date-time":"2014-06-09T22:40:41Z","timestamp":1402353641000},"page":"1694-1703","source":"Crossref","is-referenced-by-count":242,"title":["Internalization of isolated functional mitochondria: involvement of macropinocytosis"],"prefix":"10.1111","volume":"18","author":[{"given":"Tomoya","family":"Kitani","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Medicine Graduate School of Medical Science Kyoto Prefectural University School of Medicine Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Kami","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine Graduate School of Medical Science Kyoto Prefectural University of Medicine Kyoto 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