{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:28:49Z","timestamp":1791300529433,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,8,5]],"date-time":"2014-08-05T00:00:00Z","timestamp":1407196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SI 1281\/4-1"],"award-info":[{"award-number":["SI 1281\/4-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["HEALTH-F4-2011-282095"],"award-info":[{"award-number":["HEALTH-F4-2011-282095"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Epidermolysis bullosa acquisita (<jats:styled-content style=\"fixed-case\">EBA<\/jats:styled-content>) is an autoimmune subepidermal blistering disease of mucous membranes and the skin caused by autoantibodies against collagen VII. <jats:italic>In silico<\/jats:italic> and wet laboratory epitope mapping studies revealed numerous distinct epitopes recognized by <jats:styled-content style=\"fixed-case\">EBA<\/jats:styled-content> patients' autoantibodies within the non\u2010collagenous (<jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>)1 and <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>2 domains of collagen VII. However, the distribution of pathogenic epitopes on collagen VII has not yet been described. In this study, we therefore performed an <jats:italic>in vivo<\/jats:italic> functional epitope mapping of pathogenic autoantibodies in experimental <jats:styled-content style=\"fixed-case\">EBA<\/jats:styled-content>. Animals (<jats:italic>n<\/jats:italic>\u00a0=\u00a010\/group) immunized against fragments of the <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>2 domains of collagen VII or injected with antibodies generated against the same fragments developed to different extent experimental <jats:styled-content style=\"fixed-case\">EBA<\/jats:styled-content>. Our results demonstrate that antibodies targeting multiple, distinct epitopes distributed over the entire <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>1, but not <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>2 domain of collagen VII induce blistering skin disease <jats:italic>in vivo<\/jats:italic>. Our present findings have crucial implications for the development of antigen\u2010specific B\u2010 and T cell\u2010targeted therapies in <jats:styled-content style=\"fixed-case\">EBA<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jcmm.12338","type":"journal-article","created":{"date-parts":[[2014,8,5]],"date-time":"2014-08-05T11:02:30Z","timestamp":1407236550000},"page":"1727-1739","source":"Crossref","is-referenced-by-count":13,"title":["Blister\u2010inducing antibodies target multiple epitopes on collagen VII in mice"],"prefix":"10.1111","volume":"18","author":[{"given":"Kinga","family":"Csorba","sequence":"first","affiliation":[{"name":"Department of Dermatology University of Freiburg Freiburg Germany"},{"name":"Faculty of Biology University of Freiburg Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mircea Teodor","family":"Chiriac","sequence":"additional","affiliation":[{"name":"Molecular Biology Center Interdisciplinary Research Institute on Bio\u2010Nano\u2010Sciences and the Department of Biology Babes\u2010Bolyai University Cluj\u2010Napoca Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florina","family":"Florea","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Freiburg Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miruna Georgiana","family":"Ghinia","sequence":"additional","affiliation":[{"name":"Molecular Biology Center Interdisciplinary Research Institute on Bio\u2010Nano\u2010Sciences and the Department of Biology Babes\u2010Bolyai University Cluj\u2010Napoca Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilia","family":"Licarete","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Freiburg Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreea","family":"Rados","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Freiburg Freiburg Germany"},{"name":"Molecular Biology Center Interdisciplinary Research Institute on Bio\u2010Nano\u2010Sciences and the Department of Biology Babes\u2010Bolyai University Cluj\u2010Napoca Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Sas","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Freiburg Freiburg Germany"},{"name":"Molecular Biology Center Interdisciplinary Research Institute on Bio\u2010Nano\u2010Sciences and the Department of Biology Babes\u2010Bolyai University Cluj\u2010Napoca Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vlad","family":"Vuta","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Freiburg Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassian","family":"Sitaru","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Freiburg Freiburg Germany"},{"name":"Center for Biological Signalling Studies (BIOSS) University of Freiburg Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12365129"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12560795"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12277331"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0593-62"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.112.21.3641"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI34292"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198404193101602"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200521386"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2002.01772.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2004.11.010"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12272402"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-13-16"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.131.2.551"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100180200"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203247200"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)31984-1"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64182-X"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700254"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.5.3461"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901412"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2010.317"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1674"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.10.6514"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2007.08388.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2157"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4023"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2007.00564.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116774"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12374333"},{"key":"e_1_2_7_31_1","first-page":"623","article-title":"A novel variant of acquired epidermolysis bullosa with autoantibodies against the central triple\u2010helical domain of type VII collagen","volume":"77","author":"Tanaka H","year":"1997","journal-title":"Lab Investig J Tech Methods Pathol"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2004.05933.x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2011.03.003"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.061212"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-009-0256-3"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-227-8_25"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-008-0366-7"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2002.01720.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2857"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2009.00959.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100796"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2006.03115.x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0022-202x.2001.01683.x"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/35100573"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1838"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2009.12.010"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.12338","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.12338","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.12338","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.12338","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T20:33:51Z","timestamp":1696278831000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.12338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,5]]},"references-count":45,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["10.1111\/jcmm.12338"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.12338","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,5]]}}}