{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T14:27:18Z","timestamp":1774103238577,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,6,28]],"date-time":"2014-06-28T00:00:00Z","timestamp":1403913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81370473"],"award-info":[{"award-number":["81370473"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Jiangsu Province of China","award":["BK20131388"],"award-info":[{"award-number":["BK20131388"]}]},{"name":"New Century Excellent Talents of MOE","award":["NCET-13-0870"],"award-info":[{"award-number":["NCET-13-0870"]}]},{"name":"The Scientific Research Project of Jiangsu Provincial Department of health","award":["H201342"],"award-info":[{"award-number":["H201342"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>irschsprung's disease (<jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>), a congenital gastrointestinal disorder, is one of the most common causes of neonatal bowel obstruction. Without an early screening and diagnosis, some patients develop serious complications, such as toxic megacolon or acute enterocolitis. We sought to identify specific serum micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) that can serve as novel early, non\u2010invasive screening signature and then to test their specificity and sensitivity in diagnosing <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>irschsprung's disease. We obtained serum samples from 95 <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> cases and 104 matched controls. An initial screening of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression was performed through TaqMan Low Density Array. The candidate mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were validated by individual reverse transcription quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> arranged in the training and a two\u2010stage validation set. Additional double\u2010blind testing was performed in 23 patients with clinically suspected <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> to evaluate the diagnostic value and accuracy of the serum mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> profile in predicting <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>. Following a multi\u2010stage evaluation approach, five mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were significantly increased in <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> cases compared with controls. The areas under the receiver operating characteristic (<jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content>) curve of this five\u2010serum mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> signature were 0.895, 0.893 and 0.925 in training set and two validation sets, respectively. The accuracy rate of the five\u2010mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> profile as <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> signature was 82.6%, which, in the double\u2010blind testing set, was markedly higher than that of contrast enema (70%), the most commonly used test performed to diagnose <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>. Our results indicate that a five\u2010serum mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> signature may be linked to <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>, representing a potential, novel, non\u2010invasive diagnostic approach for early screening of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jcmm.12348","type":"journal-article","created":{"date-parts":[[2014,6,28]],"date-time":"2014-06-28T07:31:31Z","timestamp":1403940691000},"page":"1580-1587","source":"Crossref","is-referenced-by-count":29,"title":["Specific serum micro<scp>RNA<\/scp> profile in the molecular diagnosis of <scp>H<\/scp>irschsprung's disease"],"prefix":"10.1111","volume":"18","author":[{"given":"Weibing","family":"Tang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology School of Public Health Nanjing Medical University Nanjing 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