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Disadvantages showed in conventional lung cancer therapy probably because of its existence. In this study, lung cancer cell line A549 cells propagated as spheroid bodies (named as A549 sphere cells) in growth factors\u2010defined serum\u2010free medium. A549 sphere cells displayed <jats:styled-content style=\"fixed-case\">CSC<\/jats:styled-content> properties, including chemo\u2010resistance, increased proportion of G0\/G1 cells, slower proliferation rate, ability of differentiation and enhanced tumour formation ability <jats:italic>in vivo<\/jats:italic>. Oncolytic adenovirus <jats:styled-content style=\"fixed-case\">ZD<\/jats:styled-content>55 carrying <jats:styled-content style=\"fixed-case\">EGFP<\/jats:styled-content> gene, <jats:styled-content style=\"fixed-case\">ZD<\/jats:styled-content>55\u2010<jats:styled-content style=\"fixed-case\">EGFP<\/jats:styled-content>, infected A549 sphere cells and inhibited cell growth. Tumour necrosis factor\u2010related apoptosis\u2010inducing ligand (<jats:styled-content style=\"fixed-case\">TRAIL<\/jats:styled-content>) armed oncolytic adenovirus, <jats:styled-content style=\"fixed-case\">ZD<\/jats:styled-content>55\u2010<jats:styled-content style=\"fixed-case\">TRAIL<\/jats:styled-content>, exhibited enhanced cytotoxicity and induced A549 sphere cells apoptosis through mitochondrial pathway. Moreover, small molecules embelin, <jats:styled-content style=\"fixed-case\">LY<\/jats:styled-content>294002 and resveratrol improved the cytotoxicity of <jats:styled-content style=\"fixed-case\">ZD<\/jats:styled-content>55\u2010<jats:styled-content style=\"fixed-case\">TRAIL<\/jats:styled-content>. In the A549 sphere cells xenograft models, <jats:styled-content style=\"fixed-case\">ZD<\/jats:styled-content>55\u2010<jats:styled-content style=\"fixed-case\">TRAIL<\/jats:styled-content> significantly inhibited tumour growth and improved survival status of mice. These results suggested that gene armed oncolytic adenovirus is a potential approach for lung cancer therapy through targeting <jats:styled-content style=\"fixed-case\">LCSC<\/jats:styled-content>s.<\/jats:p>","DOI":"10.1111\/jcmm.12397","type":"journal-article","created":{"date-parts":[[2015,2,16]],"date-time":"2015-02-16T08:11:32Z","timestamp":1424074292000},"page":"915-923","source":"Crossref","is-referenced-by-count":39,"title":["Targeting lung cancer stem\u2010like cells with <scp>TRAIL<\/scp> gene armed oncolytic adenovirus"],"prefix":"10.1111","volume":"19","author":[{"given":"Yu","family":"Yang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haineng","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cell Biology 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