{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T13:42:41Z","timestamp":1740145361180,"version":"3.37.3"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,10,6]],"date-time":"2014-10-06T00:00:00Z","timestamp":1412553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81371601"],"award-info":[{"award-number":["81371601"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although <jats:sup>18<\/jats:sup>F\u2010fluorodeoxyglucose (<jats:sup>18<\/jats:sup>F\u2010<jats:styled-content style=\"fixed-case\">FDG<\/jats:styled-content>) uptake can be used for the non\u2010invasive detection and monitoring of allograft rejection by activated leucocytes, this non\u2010specific accumulation is easily impaired by immunosuppressants. Our aim was to evaluate a <jats:sup>131<\/jats:sup>I\u2010radiolabelled anti\u2010Toll\u2010like receptor 5 (<jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>5) mAb for non\u2010invasive <jats:italic>in vivo<\/jats:italic> graft visualization and quantification in allogeneic transplantation mice model, compared with the non\u2010specific radiotracer <jats:sup>18<\/jats:sup>F\u2010<jats:styled-content style=\"fixed-case\">FDG<\/jats:styled-content> under using of immunosuppressant. Labelling, binding, and stability studies were performed. <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice transplanted with C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 skin grafts, with or without rapamycin treatment (named as allo\u2010treated group or allo\u2010rejection group), were injected with <jats:sup>131<\/jats:sup>I\u2010anti\u2010<jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>5 mAb, <jats:sup>18<\/jats:sup>F\u2010<jats:styled-content style=\"fixed-case\">FDG<\/jats:styled-content>, or mouse isotype <jats:sup>131<\/jats:sup>I\u2010IgG, respectively. Whole\u2010body phosphor\u2010autoradiography and <jats:italic>ex vivo<\/jats:italic> biodistribution studies were obtained. Whole\u2010body phosphor\u2010autoradiography showed <jats:sup>131<\/jats:sup>I\u2010anti\u2010<jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>5 mAb uptake into organs that were well perfused with blood at 1\u00a0hr and showed clear graft images from 12\u00a0hrs onwards. The <jats:sup>131<\/jats:sup>I\u2010anti\u2010<jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>5 mAb had significantly higher graft uptake and target\u2010to\u2010non\u2010target ratio in the allo\u2010treated group, as determined by semi\u2010quantification of phosphor\u2010autoradiography images; these results were consistent with <jats:italic>ex vivo<\/jats:italic> biodistribution studies. However, high <jats:sup>18<\/jats:sup>F\u2010<jats:styled-content style=\"fixed-case\">FDG<\/jats:styled-content> uptake was not observed in the allo\u2010treated group. The highest allograft\u2010skin\u2010to\u2010native\u2010skin ratio (A:N) of <jats:sup>131<\/jats:sup>I\u2010anti\u2010<jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>5 mAb uptake was significantly higher than the ratio for <jats:sup>18<\/jats:sup>F\u2010<jats:styled-content style=\"fixed-case\">FDG<\/jats:styled-content> (7.68 <jats:italic>versus<\/jats:italic> 1.16, respectively). <jats:sup>131<\/jats:sup>I\u2010anti\u2010<jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>5 mAb uptake in the grafts significantly correlated with <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>5 expression in the allograft area. The accumulation of <jats:sup>131<\/jats:sup>I\u2010IgG was comparable in both groups. We conclude that radiolabelled anti\u2010<jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>5 mAb is capable of detecting allograft with high target specificity after treatment with the immunosuppressive drug rapamycin.<\/jats:p>","DOI":"10.1111\/jcmm.12423","type":"journal-article","created":{"date-parts":[[2014,10,6]],"date-time":"2014-10-06T09:19:25Z","timestamp":1412587165000},"page":"2437-2444","source":"Crossref","is-referenced-by-count":4,"title":["Non\u2010invasive imaging of allogeneic transplanted skin graft by <sup>131<\/sup>I\u2010anti\u2010TLR5 mAb"],"prefix":"10.1111","volume":"18","author":[{"given":"Hukui","family":"Sun","sequence":"first","affiliation":[{"name":"Key Laboratory for Experimental Teratology of the Ministry of Education and Institute of Experimental Nuclear Medicine School of Medicine Shandong University Ji'nan Shandong 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