{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:27:55Z","timestamp":1791268075831,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,2,16]],"date-time":"2015-02-16T00:00:00Z","timestamp":1424044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"the Taiwan Ministry of Science and Technology","doi-asserted-by":"crossref","award":["MOST 103-2628-B-010-003-MY4"],"award-info":[{"award-number":["MOST 103-2628-B-010-003-MY4"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004663","name":"the Taiwan Ministry of Science and Technology","doi-asserted-by":"crossref","award":["MOST 100-2911-I-009-101"],"award-info":[{"award-number":["MOST 100-2911-I-009-101"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Yen Tjing Ling"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cancer metastasis occurs\n                    <jats:italic>via<\/jats:italic>\n                    a progress involving abnormal cell migration. Cell migration, a dynamic physical process, is controlled by the cytoskeletal system, which includes the dynamics of actin organization and cellular adhesive organelles, focal adhesions (\n                    <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content>\n                    s). However, it is not known whether the organization of actin cytoskeletal system has a regulatory role in the physiologically relevant aspects of cancer metastasis. In the present studies, it was found that lung adenocarcinoma cells isolated from the secondary lung cancer of the lymph nodes, H1299 cells, show specific dynamics in terms of the actin cytoskeleton and\n                    <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content>\n                    s. This results in a higher level of mobility and this is regulated by an immature\n                    <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content>\n                    component, \u03b2\u2010\n                    <jats:styled-content style=\"fixed-case\">PIX<\/jats:styled-content>\n                    (\n                    <jats:styled-content style=\"fixed-case\">PAK<\/jats:styled-content>\n                    \u2010interacting exchange factor\u2010\u03b2). In H1299 cells, \u03b2\u2010\n                    <jats:styled-content style=\"fixed-case\">PIX<\/jats:styled-content>\n                    's activity was found not to be down\u2010regulated by sequestration onto stress fibres, as the cells did not bundle actin filaments into stress fibres. Thus, \u03b2\u2010\n                    <jats:styled-content style=\"fixed-case\">PIX<\/jats:styled-content>\n                    mainly remained localized at\n                    <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content>\n                    s, which allowed maturation of nascent adhesions into focal complexes; this resulted in actin polymerization, increased actin network integrity, changes in the intracellular microrheology at the peripheral of the cell, and cell polarity, which in turn regulated cell migration. Perturbation of \u03b2\u2010\n                    <jats:styled-content style=\"fixed-case\">PIX<\/jats:styled-content>\n                    caused an inhibition of cell migration, including migration velocity, accumulated distance and directional persistence. Our results demonstrate the importance of \u03b2\u2010\n                    <jats:styled-content style=\"fixed-case\">PIX<\/jats:styled-content>\n                    to the regulation of high mobility of lung adenocarcinoma cell line H1299 and that this occurs\n                    <jats:italic>via<\/jats:italic>\n                    regulation of\n                    <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content>\n                    dynamics, changes in actin cytoskeleton organization and cell polarity.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.12441","type":"journal-article","created":{"date-parts":[[2015,2,16]],"date-time":"2015-02-16T03:11:49Z","timestamp":1424056309000},"page":"934-947","source":"Crossref","is-referenced-by-count":23,"title":["\u03b2\u2010\n                    <scp>PIX<\/scp>\n                    controls intracellular viscoelasticity to regulate lung cancer cell migration"],"prefix":"10.1111","volume":"19","author":[{"given":"Helen Wenshin","family":"Yu","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology National Yang\u2010Ming University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin\u2010Quan","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Biophotonics National Yang\u2010Ming University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi\u2010Ming","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology National Yang\u2010Ming University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching\u2010Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Basic Medical Sciences National Cheng Kung University Tainan Taiwan"},{"name":"Department of Physiology National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur","family":"Chiou","sequence":"additional","affiliation":[{"name":"Institute of Biophotonics National Yang\u2010Ming University Taipei Taiwan"},{"name":"Biophotonics &amp; Molecular Imaging Research Center National Yang\u2010Ming University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang\u2010Kao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Anatomy National Cheng Kung University Tainan Taiwan"},{"name":"Center for Neurotrauma and Neuroregeneration Taipei Medical University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010Jer","family":"Tang","sequence":"additional","affiliation":[{"name":"Institute of Basic Medical Sciences National Cheng Kung University Tainan Taiwan"},{"name":"Department of Physiology National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Cheng","family":"Kuo","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology National Yang\u2010Ming University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200209006"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb873"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(03)00397-6"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2012.02.007"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201307106"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00347.2003"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1075"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81280-5"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0402-e97"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.05.029"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.04.110188.002415"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00971-6"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.11.110195.002115"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.11.110195.003001"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0807-858"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12054"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2216"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0411-344"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107704200"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200509096"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201003091"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201003057"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3104"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(03)00004-1"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-005-1085-6"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2010.11.010"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.19.1.011008"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007054"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077384"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1155\/2004\/482680"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.07.078"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104101200"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.104.045476"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002490050213"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-4073"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046609"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0804573105"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1207326109"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.084988"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.050708.133724"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2011.436"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.6.2015"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E04-06-0485"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003970000094"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80019-2"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.10.10.3097"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025306"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042773"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.12441","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.12441","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.12441","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.12441","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T16:13:03Z","timestamp":1696263183000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.12441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,16]]},"references-count":48,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1111\/jcmm.12441"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.12441","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,16]]}}}