{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:55:45Z","timestamp":1787241345524,"version":"build-2736575974"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,3,6]],"date-time":"2015-03-06T00:00:00Z","timestamp":1425600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81370255, 81070325, 81170799"],"award-info":[{"award-number":["81370255, 81070325, 81170799"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003399","name":"Shanghai Science and Technology Committee","doi-asserted-by":"crossref","award":["10DZ1976000"],"award-info":[{"award-number":["10DZ1976000"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Chemerin is a novel adipocyte\u2010derived factor that induces insulin resistance in skeletal muscle. However, the effect of chemerin on skeletal muscle mitochondrial function has received little attention. In the present study, we investigated whether mitochondrial dysfunction is involved in the pathogenesis of chemerin\u2010mediated insulin resistance. In this study, we used recombinant adenovirus to express murine chemerin in C57\n                    <jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\n                    \/6 mice. The mitochondrial function and structure were evaluated in isolated soleus muscles from mice. The oxidative mechanism of mitochondrial dysfunction in cultured C2C12 myotubes exposed to recombinant chemerin was analysed by western blotting, immunofluorescence and quantitative real\u2010time polymerase chain reaction. The overexpression of chemerin in mice reduced the muscle mitochondrial content and increased mitochondrial autophagy, as determined by the increased conversion of\n                    <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\n                    3\u2010I to\n                    <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\n                    3\u2010II and higher expression levels of Beclin1 and autophagy\u2010related protein\u20105 and 7. The chemerin treatment of C2C12 myotubes increased the generation of mitochondrial reactive oxygen species, concomitant with a reduced mitochondrial membrane potential and increased the occurrence of mitochondrial protein carbonyls and mitochondrial\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    deletions. Knockdown of the expression of chemokine\u2010like receptor 1 or the use of mitochondria\u2010targeting antioxidant Mito\u2010\n                    <jats:styled-content style=\"fixed-case\">TEMPO<\/jats:styled-content>\n                    restored the mitochondrial dysfunction induced by chemerin. Furthermore, chemerin exposure in C2C12 myotubes not only reduced the insulin\u2010stimulated phosphorylation of protein kinase B (\n                    <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>\n                    ) but also dephosphorylated forkhead box O3\u03b1 (FoxO3\u03b1). Chemerin\u2010induced mitochondrial autophagy likely through an\n                    <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>\n                    \u2010FoxO3\u03b1\u2010dependent signalling pathway. These findings provide direct evidence that chemerin may play an important role in regulating mitochondrial remodelling and function in skeletal muscle.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.12487","type":"journal-article","created":{"date-parts":[[2015,3,6]],"date-time":"2015-03-06T04:41:35Z","timestamp":1425616895000},"page":"986-995","source":"Crossref","is-referenced-by-count":38,"title":["Chemerin\u2010induced mitochondrial dysfunction in skeletal muscle"],"prefix":"10.1111","volume":"19","author":[{"given":"Qihai","family":"Xie","sequence":"first","affiliation":[{"name":"State Key Laboratory of Medical Genomics Shanghai Key Laboratory of Hypertension Department of Hypertension Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Endocrine and Metabolic Diseases Shanghai Institute of Endocrine and Metabolic Diseases Shanghai Clinical Center for Endocrine and Metabolic Diseases Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenglin","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medical Genomics Shanghai Key Laboratory of Hypertension Department of Hypertension Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penghao","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medical Genomics Shanghai Key Laboratory of Hypertension Department of Hypertension Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Endocrine and Metabolic Diseases Shanghai Institute of Endocrine and Metabolic Diseases Shanghai Clinical Center for Endocrine and Metabolic Diseases Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weili","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medical Genomics Shanghai Key Laboratory of Hypertension Department of Hypertension Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingjin","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medical Genomics Shanghai Key Laboratory of Hypertension Department of Hypertension Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai 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