{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:38:07Z","timestamp":1788871087277,"version":"build-2803163510"},"reference-count":71,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,2,20]],"date-time":"2015-02-20T00:00:00Z","timestamp":1424390400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["FOR1075 TP3"],"award-info":[{"award-number":["FOR1075 TP3"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2015,5]]},"abstract":"Abstract<\/jats:title>\n \n Connective tissue growth factor (\n CTGF<\/jats:styled-content>\n ) induces extracellular matrix (\n ECM<\/jats:styled-content>\n ) synthesis and contractility in human trabecular meshwork (\n HTM<\/jats:styled-content>\n ) cells. Both processes are involved in the pathogenesis of primary open\u2010angle glaucoma. To date, little is known about regulation and function of\n CTGF<\/jats:styled-content>\n expression in the trabecular meshwork (\n TM<\/jats:styled-content>\n ). Therefore, we analysed the effects of different aqueous humour proteins and stressors on\n CTGF<\/jats:styled-content>\n expression in\n HTM<\/jats:styled-content>\n cells.\n HTM<\/jats:styled-content>\n cells from three different donors were treated with endothelin\u20101, insulin\u2010like growth factor (\n IGF<\/jats:styled-content>\n )\u20101, angiotensin\u2010II,\n \n \n H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n <\/jats:roman>\n <\/jats:styled-content>\n and heat shock and were analysed by immunohistochemistry, real\u2010time\n RT<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n and Western blotting. Viability after\n \n \n H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n <\/jats:roman>\n <\/jats:styled-content>\n treatment was measured in\n CTGF<\/jats:styled-content>\n silenced\n HTM<\/jats:styled-content>\n \u2010N cells and their controls. Latrunculin A reduced expression of\n CTGF<\/jats:styled-content>\n by about 50% compared to untreated\n HTM<\/jats:styled-content>\n cells, whereas endothelin\u20101,\n IGF<\/jats:styled-content>\n \u20101, angiotensin\u2010II, heat shock and oxidative stress led to a significant increase. Silencing of\n CTGF<\/jats:styled-content>\n resulted in a delayed expression of \u03b1B\u2010crystallin and in reduced cell viability in comparison to the controls after oxidative stress. Conversely,\n CTGF<\/jats:styled-content>\n treatment led to a higher cell viability rate after\n \n \n H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n <\/jats:roman>\n <\/jats:styled-content>\n treatment.\n CTGF<\/jats:styled-content>\n expression is induced by factors that have been linked to glaucoma. An increased level of\n CTGF<\/jats:styled-content>\n appears to protect\n TM<\/jats:styled-content>\n cells against damage induced by stress. The beneficial effect of\n CTGF<\/jats:styled-content>\n for viability of\n TM<\/jats:styled-content>\n cells is likely associated with the effects on increased\n ECM<\/jats:styled-content>\n synthesis and higher contractility of the\n TM<\/jats:styled-content>\n , thereby contributing to reduced aqueous humour outflow facility causing increased intraocular pressure.\n <\/jats:p>","DOI":"10.1111\/jcmm.12492","type":"journal-article","created":{"date-parts":[[2015,2,20]],"date-time":"2015-02-20T05:55:39Z","timestamp":1424411739000},"page":"1010-1020","source":"Crossref","is-referenced-by-count":14,"title":["The regulation of connective tissue growth factor expression influences the viability of human trabecular meshwork cells"],"prefix":"10.1111","volume":"19","author":[{"given":"Sabrina","family":"Kuespert","sequence":"first","affiliation":[{"name":"Institute of Human Anatomy and Embryology University of Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Junglas","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy and Embryology University of Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara M.","family":"Braunger","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy and Embryology University of Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernst R.","family":"Tamm","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy and Embryology University of Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"Fuchshofer","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy and Embryology University of Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.80.5.389"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.120.6.714"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.121.1.48"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/S0002-9394(00)00538-9","article-title":"The Advanced Glaucoma Intervention Study (AGIS): 7. 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