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Affected patients suffer from hepatic disorders and severe neurological defects. Experimental studies in mutant mice in which the copper\u2010transporting <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>ase gene (<jats:italic>Atp7b<\/jats:italic>) is disrupted revealed a drastic, time\u2010dependent accumulation of hepatic copper that is accompanied by formation of regenerative nodes resembling cirrhosis. Therefore, these mice represent an excellent exploratory model for <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>ilson's disease. However, the precise time course in hepatic copper accumulation and its impact on other trace metals within the liver is yet poorly understood. We have recently established novel laser ablation inductively coupled plasma mass spectrometry protocols allowing quantitative metal imaging in human and murine liver tissue with high sensitivity, spatial resolution, specificity and quantification ability. By use of these techniques, we here aimed to comparatively analyse hepatic metal content in wild\u2010type and <jats:italic>Atp7b<\/jats:italic> deficient mice during ageing. We demonstrate that the age\u2010dependent accumulation of hepatic copper is strictly associated with a simultaneous increase in iron and zinc, while the intrahepatic concentration and distribution of other metals or metalloids is not affected. The same findings were obtained in well\u2010defined human liver samples that were obtained from patients suffering from <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>ilson's disease. We conclude that in <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>ilson's disease the imbalances of hepatic copper during ageing are closely correlated with alterations in intrahepatic iron and zinc content.<\/jats:p>","DOI":"10.1111\/jcmm.12497","type":"journal-article","created":{"date-parts":[[2015,2,20]],"date-time":"2015-02-20T10:55:29Z","timestamp":1424429729000},"page":"806-814","source":"Crossref","is-referenced-by-count":48,"title":["Laser ablation inductively coupled plasma mass spectrometry imaging of metals in experimental and clinical Wilson's disease"],"prefix":"10.1111","volume":"19","author":[{"given":"Sorina Georgiana","family":"Boaru","sequence":"first","affiliation":[{"name":"Institute of Molecular Pathobiochemistry, Experimental Gene Therapie and Clinical Chemistry RWTH Aachen University Hospital Aachen Aachen 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