{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T16:51:01Z","timestamp":1779123061337,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,3,27]],"date-time":"2015-03-27T00:00:00Z","timestamp":1427414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"crossref","award":["J3-2330"],"award-info":[{"award-number":["J3-2330"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adrenergic stimulation is important for osteoclast differentiation and bone resorption. Previous research shows that this happens through \u03b22\u2010adrenergic receptor (<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content>), but there are conflicting evidence on presence and role of \u03b12A\u2010<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> in bone. The aim of this study was to investigate the presence of \u03b12A\u2010<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> and its involvement in neuro\u2010endocrine signalling of bone remodelling in humans. Real\u2010time polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) and immunohistochemistry were used to investigate \u03b12A\u2010<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> receptor presence and localization in bone cells. Functionality of rs553668 and rs1800544 single nucleotide polymorphism <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>s located in \u03b12A\u2010<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> gene was analysed by <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> expression on bone samples and luciferase reporter assay in human osteosarcoma <jats:styled-content style=\"fixed-case\">HOS<\/jats:styled-content> cells. Using real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, genetic association study between rs553668 A&gt;G and rs1800544 C&gt;G <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>s and major bone markers was performed on 661 Slovenian patients with osteoporosis. \u03b12A\u2010<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> is expressed in osteoblasts and lining cells but not in osteocytes. <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content> rs553668 has a significant influence on \u03b12A\u2010<jats:styled-content style=\"fixed-case\">AR mRNA<\/jats:styled-content> level in human bone samples through the stability of <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>. \u03b12A\u2010<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> gene locus associates with important bone remodelling markers (<jats:styled-content style=\"fixed-case\">BMD<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> CTX<\/jats:styled-content>, Cathepsin K and <jats:styled-content style=\"fixed-case\">pOC<\/jats:styled-content>). The results of this study are providing comprehensive new evidence that \u03b12A\u2010<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> is involved in neuro\u2010endocrine signalling of bone turnover and development of osteoporosis. As shown by our results the neurological signalling is mediated through osteoblasts and result in bone resorption. Genetic study showed association of <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>s in \u03b12A\u2010<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> gene locus with bone remodelling markers, identifying the individuals with higher risk of development of osteoporosis.<\/jats:p>","DOI":"10.1111\/jcmm.12505","type":"journal-article","created":{"date-parts":[[2015,3,27]],"date-time":"2015-03-27T22:20:20Z","timestamp":1427494820000},"page":"1520-1529","source":"Crossref","is-referenced-by-count":24,"title":["<scp>ADRA<\/scp>2A is involved in neuro\u2010endocrine regulation of bone resorption"],"prefix":"10.1111","volume":"19","author":[{"given":"Vid","family":"Mlakar","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry Faculty of Pharmacy University of Ljubljana Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Jurkovic 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