{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T09:43:02Z","timestamp":1770889382892,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,3,30]],"date-time":"2015-03-30T00:00:00Z","timestamp":1427673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL089824"],"award-info":[{"award-number":["R01HL089824"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL110740"],"award-info":[{"award-number":["R01HL110740"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL105176"],"award-info":[{"award-number":["R01HL105176"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The chemokine (C\u2010X\u2010C motif) receptor 4 (<jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4) is expressed on native cardiomyocytes and can modulate isolated cardiomyocyte contractility. This study examines the role of <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 in cardiomyocyte response to ischaemia\u2010reperfusion (I\/R) injury. Isolated adult rat ventricular cardiomyocytes were subjected to hypoxia\/reoxygenation (H\/R) to simulate I\/R injury. In response to H\/R injury, the decrease in <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 expression was associated with dysfunctional energy metabolism indicated by an increased adenosine diphosphate\/adenosine triphosphate (ADP\/ATP) ratio. <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4\u2010overexpressing cardiomyocytes were used to determine whether such overexpression (<jats:styled-content style=\"fixed-case\">OE<\/jats:styled-content>) can prevent bio\u2010energetic disruption\u2010associated cell death. <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 <jats:styled-content style=\"fixed-case\">OE<\/jats:styled-content> was performed with adenoviral infection with <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 encoding\u2010gene or non\u2010translated nucleotide sequence (Control). The increased <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 expression was observed in cardiomyocytes post <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4\u2010adenovirus transduction and this <jats:styled-content style=\"fixed-case\">OE<\/jats:styled-content> significantly reduced the cardiomyocyte contractility under basal conditions. Although the same extent of H\/R\u2010provoked cytosolic calcium overload was measured, the hydrogen peroxide\u2010induced decay of mitochondrial membrane potential was suppressed in <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 <jats:styled-content style=\"fixed-case\">OE<\/jats:styled-content> group compared with control group, and the mitochondrial swelling was significantly attenuated in <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 group, implicating that <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 <jats:styled-content style=\"fixed-case\">OE<\/jats:styled-content> prevents permeability transition pore opening exposure to overload calcium. Interestingly, this <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4\u2010induced mitochondrial protective effect is associated with the enhanced signal transducer and activator of transcription 3 (expression in mitochondria. Consequently, in the presence of H\/R, mitochondrial dysfunction was mitigated and cardiomyocyte death was decreased to 65% in the <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 <jats:styled-content style=\"fixed-case\">OE<\/jats:styled-content> group as compared with the control group. I\/R injury leads to the reduction in <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 in cardiomyocytes associated with the dysfunctional energy metabolism, and <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 <jats:styled-content style=\"fixed-case\">OE<\/jats:styled-content> can alleviate mitochondrial dysfunction to improve cardiomyocyte survival.<\/jats:p>","DOI":"10.1111\/jcmm.12554","type":"journal-article","created":{"date-parts":[[2015,3,31]],"date-time":"2015-03-31T00:18:14Z","timestamp":1427761094000},"page":"1825-1835","source":"Crossref","is-referenced-by-count":17,"title":["<scp>CXCR<\/scp>4 attenuates cardiomyocytes mitochondrial dysfunction to resist ischaemia\u2010reperfusion injury"],"prefix":"10.1111","volume":"19","author":[{"given":"Wen\u2010Feng","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Pathology &amp; Lab Medicine College of Medicine University of Cincinnati Cincinnati OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Lab Medicine College of Medicine University of Cincinnati Cincinnati OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Lab Medicine College of Medicine University of Cincinnati Cincinnati OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia\u2010Liang","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Lab Medicine College of Medicine University of Cincinnati Cincinnati OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Liang","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Lab Medicine College of Medicine University of Cincinnati Cincinnati OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guan\u2010Sheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; Cell Biophysics College of Medicine University of Cincinnati Cincinnati OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"De\u2010Hua","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Lab Medicine College of Medicine University of Cincinnati Cincinnati OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Li","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Lab Medicine College of Medicine University of Cincinnati Cincinnati OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Paul","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Lab Medicine College of Medicine University of Cincinnati Cincinnati OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meifeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Lab Medicine College of Medicine University of Cincinnati Cincinnati OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald W.","family":"Millard","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; Cell Biophysics College of Medicine University of Cincinnati Cincinnati OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yigang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Lab Medicine College of Medicine University of Cincinnati Cincinnati OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,3,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra071667"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI62874"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvq129"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1194396"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200737118"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0b013e3181f713fe"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2006.08.008"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(00)00142-5"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.090451"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00365.2011"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2012.05.016"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.112.265660"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2007.11.010"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvs119"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.112.279935"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-010-0106-3"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2006.12.012"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046158"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-013-0377-6"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-013-0382-9"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.113.302095"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.761_9.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-014-0440-y"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.378984"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1164551"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-010-0124-1"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.255604"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161281939131127115940"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.164699"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2008.02.005"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.12554","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.12554","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.12554","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.12554","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T21:13:20Z","timestamp":1696281200000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.12554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,30]]},"references-count":30,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1111\/jcmm.12554"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.12554","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,30]]}}}