{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T01:22:41Z","timestamp":1773192161991,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,3,5]],"date-time":"2015-03-05T00:00:00Z","timestamp":1425513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To explore the impact of myocardial injection of mesenchymal stem cells (<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s) and specific recombinant human <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content><jats:sub>165<\/jats:sub> (<jats:styled-content style=\"fixed-case\">hVEGF<\/jats:styled-content><jats:sub>165<\/jats:sub>) plasmid on collagen remodelling in rats with furazolidone induced dilated cardiomyopathy (<jats:styled-content style=\"fixed-case\">DCM<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">DCM<\/jats:styled-content> was induced by furazolidone (0.3\u00a0mg\/bodyweight (g)\/day per gavage for 8\u00a0weeks). Rats were then divided into four groups: (<jats:italic>i<\/jats:italic>) <jats:styled-content style=\"fixed-case\">PBS<\/jats:styled-content> group (<jats:italic>n<\/jats:italic>\u00a0=\u00a018): rats received equal volume myocardial <jats:styled-content style=\"fixed-case\">PBS<\/jats:styled-content> injection; (<jats:italic>ii<\/jats:italic>) <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s group (<jats:italic>n<\/jats:italic>\u00a0=\u00a017): 100\u00a0\u03bcl culture medium containing 10<jats:sup>5<\/jats:sup> <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s were injected into four sites of left ventricular free wall (25\u00a0\u03bcl per site); (<jats:italic>iii<\/jats:italic>) <jats:styled-content style=\"fixed-case\">GENE<\/jats:styled-content> group (<jats:italic>n<\/jats:italic>\u00a0=\u00a018): <jats:styled-content style=\"fixed-case\">pCMV<\/jats:styled-content>en\u2010<jats:styled-content style=\"fixed-case\">MLC<\/jats:styled-content>2v\u2010<jats:styled-content style=\"fixed-case\">EGFP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content><jats:sub>165<\/jats:sub> plasmid [5\u00a0\u00d7\u00a0109\u00a0pfu (0.2\u00a0ml)] were injected into four sites of left ventricular free wall (0.05\u00a0ml per site)] and (<jats:italic>iv<\/jats:italic>) <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s+<jats:styled-content style=\"fixed-case\">GENE<\/jats:styled-content> group (<jats:italic>n<\/jats:italic>\u00a0=\u00a017): rats received both myocardial <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">pCMV<\/jats:styled-content>en\u2010<jats:styled-content style=\"fixed-case\">MLC<\/jats:styled-content>2v\u2010<jats:styled-content style=\"fixed-case\">EGFP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content><jats:sub>165<\/jats:sub> plasmid injections. After 4\u00a0weeks, cardiac function was evaluated by echocardiography. Myocardial <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expressions of type I, type III collagen and transforming growth factor (<jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>)\u2010\u03b21 were detected by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. The protein expression of <jats:styled-content style=\"fixed-case\">hVEGF<\/jats:styled-content><jats:sub>165<\/jats:sub> was determined by Western blot. Myocardial protein expression of <jats:styled-content style=\"fixed-case\">hVEGF<\/jats:styled-content><jats:sub>165<\/jats:sub> was demonstrated in <jats:styled-content style=\"fixed-case\">GENE<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s+<jats:styled-content style=\"fixed-case\">GENE<\/jats:styled-content> groups. Cardiac function was improved in <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s, <jats:styled-content style=\"fixed-case\">GENE<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s+<jats:styled-content style=\"fixed-case\">GENE<\/jats:styled-content> groups. Collagen volume fraction was significantly reduced and myocardial <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b21 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression significantly down\u2010regulated in both <jats:styled-content style=\"fixed-case\">GENE<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s+<jats:styled-content style=\"fixed-case\">GENE<\/jats:styled-content> groups, collagen type I\/III ratio reduction was more significant in <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s+<jats:styled-content style=\"fixed-case\">GENE<\/jats:styled-content> group than in <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s or <jats:styled-content style=\"fixed-case\">GENE<\/jats:styled-content> group. Myocardial <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">hVEGF<\/jats:styled-content><jats:sub>165<\/jats:sub> plasmid injection improves cardiac function possibly through down\u2010regulating myocardial <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b21 expression and reducing the type I\/III collagen ratio in this DCM rat model.<\/jats:p>","DOI":"10.1111\/jcmm.12558","type":"journal-article","created":{"date-parts":[[2015,3,5]],"date-time":"2015-03-05T12:04:11Z","timestamp":1425557051000},"page":"1868-1876","source":"Crossref","is-referenced-by-count":10,"title":["Beneficial effects of intramyocardial mesenchymal stem cells and <scp>VEGF<\/scp><sub>165<\/sub> plasmid injection in rats with furazolidone induced dilated cardiomyopathy"],"prefix":"10.1111","volume":"19","author":[{"given":"Qin","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Cardiology Affiliated 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