{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T21:35:59Z","timestamp":1792704959935,"version":"4.5.0"},"reference-count":23,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T00:00:00Z","timestamp":1438905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Liver fibrosis is characterized by an exacerbated accumulation of deposition of the extracellular matrix (<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>), and the activation of hepatic stellate cells (<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>) plays a pivotal role in the development of liver fibrosis. Periostin has been shown to regulate cell adhesion, proliferation, migration and apoptosis; however, the involvement of periostin and its role in transforming growth factor (<jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>)\u2010\u03b21\u2010induced <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content> activation remains unclear. We used <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and Western blot to evaluate the expression level of periostin in hepatic fibrosis tissues and <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s, respectively. Cell proliferation was determined using the Cell Proliferation <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> BrdU kit, cell cycle was analysed by flow cytometry. The expression of \u03b1\u2010smooth muscle actin (\u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>), collagen I, <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b21, p\u2010Smad2 and p\u2010Smad3 were determined by western blot. Our study found that periostin was up\u2010regulated in liver fibrotic tissues and activated <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s. In addition, si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010periostin suppressed <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b21\u2010induced <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content> proliferation. The <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content> transfected with si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010periostin significantly inhibited <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b21\u2010induced expression levels of \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> and collagen I. Furthermore, <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b21 stimulated the expression of periostin, and si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010periostin attenuated <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b21\u2010induced Smad2\/3 activation in <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s. These results suggest that periostin may function as a novel regulator to modulate <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content> activation, potentially by promoting the <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b21\/Smad signalling pathway, and propose a strategy to target periostin for the treatment of liver fibrosis.<\/jats:p>","DOI":"10.1111\/jcmm.12636","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T05:20:12Z","timestamp":1438924812000},"page":"2462-2468","source":"Crossref","is-referenced-by-count":31,"title":["Periostin down\u2010regulation attenuates the pro\u2010fibrogenic response of hepatic stellate cells induced by <scp>TGF<\/scp>\u2010\u03b21"],"prefix":"10.1111","volume":"19","author":[{"given":"Li","family":"Hong","sequence":"first","affiliation":[{"name":"Department of Gastroenterology The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi 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