{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:58:57Z","timestamp":1792591137890,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,1,29]],"date-time":"2016-01-29T00:00:00Z","timestamp":1454025600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571875"],"award-info":[{"award-number":["81571875"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Idiopathic pulmonary fibrosis (<jats:styled-content style=\"fixed-case\">IPF<\/jats:styled-content>) is a chronic fatal lung disease characterized by aberrant accumulation of fibroblast population and deposition of extra cellular matrix. Increasing evidence support that epithelial\u2010mesenchymal transition (<jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content>) of alveolar epithelial cells is a critical process in the pathogenesis of <jats:styled-content style=\"fixed-case\">IPF<\/jats:styled-content>. Although delivery of bleomycin to induce acute lung injury is the most well\u2010studied animal model of pulmonary fibrosis, there is considerable interest to pursue other models to understand the common and\/or specific pathological mechanisms. In this study, we established a mouse model of pulmonary injury and progressive interstitial fibrosis <jats:italic>via<\/jats:italic> intraperitoneal injection of paraquat, a widely used herbicide known to cause pulmonary fibrosis in human. Using transcriptome sequencing and microarray analysis, we profiled expression of long non\u2010coding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) and identified 513 up\u2010regulated and 204 down\u2010regulated lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in paraquat\u2010induced fibrotic lung tissues. Gene ontology analysis revealed that the differentially expressed lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s are implicated in cell differentiation, epithelium morphogenesis and wound healing, pathways closely associated with <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content>. Furthermore, we identified the evolutionally conserved target genes of two up\u2010regulated lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s, uc.77 and 2700086A05Rik, as <jats:italic>Zeb2<\/jats:italic> and <jats:italic>Hoxa3<\/jats:italic>, respectively, both of which are important modulators of <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content>. Consistently, overexpression of uc.77 or 2700086A05Rik in human lung epithelial cells induced <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content> as demonstrated by changes in gene and protein expression of various <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content> markers and cell morphology. Collectively, our results uncovered a crucial role of lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in the regulation of <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content> during lung fibrosis and provide potential avenues for the discovery of novel molecular markers and therapeutic targets for <jats:styled-content style=\"fixed-case\">IPF<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jcmm.12783","type":"journal-article","created":{"date-parts":[[2016,1,29]],"date-time":"2016-01-29T21:22:58Z","timestamp":1454102578000},"page":"1234-1246","source":"Crossref","is-referenced-by-count":62,"title":["Integrated long non\u2010coding <scp>RNA<\/scp> analyses identify novel regulators of epithelial\u2010mesenchymal transition in the mouse model of pulmonary fibrosis"],"prefix":"10.1111","volume":"20","author":[{"given":"Hao","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Emergency The First Affiliated Hospital with Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Emergency The First Affiliated Hospital with Nanjing Medical University Nanjing China"},{"name":"Department of Intensive Care Unit Yixing People's Hospital Yixing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyi","family":"Qian","sequence":"additional","affiliation":[{"name":"Key Lab of Modern Toxicology Ministry of Education and Department of Toxicology School of Public Health Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Emergency The First Affiliated Hospital with Nanjing Medical University Nanjing 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