{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T23:21:14Z","timestamp":1778109674662,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,3,16]],"date-time":"2016-03-16T00:00:00Z","timestamp":1458086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC 81370473"],"award-info":[{"award-number":["NSFC 81370473"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2016,7]]},"abstract":"Abstract<\/jats:title>Hirschsprung disease (HSCR<\/jats:styled-content>) is a congenital disorder caused by the defective function of the embryonic enteric neural crest. The impaired migration of embryonic enteric neural crest plays an important role in the pathogenesis of this disease. Recent studies showed that the ARP<\/jats:styled-content>2\/3 complex and RAC<\/jats:styled-content> isoforms had effects on actin cytoskeleton remodelling, which contributes to migration. Moreover, some regulatory relationships were identified between ARP<\/jats:styled-content>2\/3 complex and RAC<\/jats:styled-content> isoforms. Although microRNA<\/jats:styled-content>s (miRNA<\/jats:styled-content>s) have been known to modulate target gene expression on the post\u2010transcriptional level, little is known about the regulation among miRNA<\/jats:styled-content>s, ARP<\/jats:styled-content>2\/3 complex and RAC<\/jats:styled-content> isoforms. Here, we report that down\u2010regulation of ARP<\/jats:styled-content>2 and ARP<\/jats:styled-content>3, two main subunits of ARP<\/jats:styled-content>2\/3 complex, suppressed migration and proliferation in 293T and SH<\/jats:styled-content>\u2010SY<\/jats:styled-content>5Y cell lines via<\/jats:italic> the inhibition of RAC<\/jats:styled-content>1 and RAC<\/jats:styled-content>2. Meanwhile, as the target genes, ARP<\/jats:styled-content>2 and ARP<\/jats:styled-content>3 are reduced by increased miR\u201024\u20101* and let\u20107a*, respectively, in 70 HSCR<\/jats:styled-content> samples as compared with 74 normal controls. Co\u2010immunoprecipitation showed that aberrant reduction in ARP<\/jats:styled-content>2 and ARP<\/jats:styled-content>3 could weaken the function of ARP<\/jats:styled-content>2\/3 complex. Our study demonstrates that the miR\u201024\u20101*\/let\u20107a*\u2010ARP<\/jats:styled-content>2\/3 complex\u2010RAC<\/jats:styled-content> isoforms pathway may represent a novel pathogenic mechanism for HSCR<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jcmm.12799","type":"journal-article","created":{"date-parts":[[2016,3,19]],"date-time":"2016-03-19T02:04:52Z","timestamp":1458353092000},"page":"1266-1275","source":"Crossref","is-referenced-by-count":29,"title":["Suppressive action of miRNA<\/scp>s to ARP<\/scp>2\/3 complex reduces cell migration and proliferation via <\/i>RAC<\/scp> isoforms in Hirschsprung disease"],"prefix":"10.1111","volume":"20","author":[{"given":"Weibing","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery Nanjing Children's Hospital Affiliated Nanjing Medical University Nanjing China"},{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology School of Public Health Nanjing Medical University 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