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Aspirin (acetylsalicylic acid, <jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content>) has antioxidant activity and has great preventive and therapeutical effect in many oxidative stress\u2010relevant diseases. Whether <jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content> can protect human melanocytes against oxidative stress needs to be further studied. Here, we investigated the potential protective effect and mechanisms of <jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content> against H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced oxidative injury in human melanocytes. Human melanocytes were pre\u2010treated with different concentrations of <jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content>, followed by exposure to 1.0 mM H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. Cell apoptosis, intracellular reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>) levels were evaluated by flow cytometry, and cell viability was determined by an Cell Counting Kit\u20108 assay. Total and phosphorylated <jats:styled-content style=\"fixed-case\">NRF<\/jats:styled-content>2 expression, <jats:styled-content style=\"fixed-case\">NRF<\/jats:styled-content>2 nuclear translocation and antioxidant response element (<jats:styled-content style=\"fixed-case\">ARE<\/jats:styled-content>) transcriptional activity were assayed with or without Nrf2\u2010si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> transfection to investigate the possible molecular mechanisms. Concomitant with an increase in viability, pre\u2010treatment of 10\u201090 \u03bcmol\/l <jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content> resulted in decreased rate of apoptotic cells, lactate dehydrogenase release and intracellular <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> levels in primary human melanocytes. Furthermore, we found <jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content> dramatically induced <jats:styled-content style=\"fixed-case\">NRF<\/jats:styled-content>2 nuclear translocation, enhanced <jats:styled-content style=\"fixed-case\">ARE<\/jats:styled-content>\u2010luciferase activity, increased both p\u2010 <jats:styled-content style=\"fixed-case\">NRF<\/jats:styled-content>2 and total <jats:styled-content style=\"fixed-case\">NRF<\/jats:styled-content>2 levels, and induced the expression of haem oxygenase\u20101 (<jats:styled-content style=\"fixed-case\">HO<\/jats:styled-content>\u20101) in human melanocytes. In addition, knockdown of Nrf2 expression or pharmacological inhibition of <jats:styled-content style=\"fixed-case\">HO<\/jats:styled-content>\u20101 abrogated the protective action of <jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content> on melanocytes against H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced cytotoxicity and apoptosis. These results suggest that <jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content> protects human melanocytes against H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced oxidative stress via Nrf2\u2010driven transcriptional activation of <jats:styled-content style=\"fixed-case\">HO<\/jats:styled-content>\u20101.<\/jats:p>","DOI":"10.1111\/jcmm.12812","type":"journal-article","created":{"date-parts":[[2016,3,12]],"date-time":"2016-03-12T07:39:19Z","timestamp":1457768359000},"page":"1307-1318","source":"Crossref","is-referenced-by-count":62,"title":["Aspirin induces Nrf2\u2010mediated transcriptional activation of haem oxygenase\u20101 in protection of human melanocytes from H<sub>2<\/sub>O<sub>2<\/sub>\u2010induced oxidative stress"],"prefix":"10.1111","volume":"20","author":[{"given":"Zhe","family":"Jian","sequence":"first","affiliation":[{"name":"Department of Dermatology Xijing Hospital Fourth Military Medical University Xi'an Shaanxi 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