{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:36:33Z","timestamp":1790026593682,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,3,14]],"date-time":"2016-03-14T00:00:00Z","timestamp":1457913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Periodontitis is a chronic inflammatory disease which is caused by bacterial infection and leads to the destruction of periodontal tissues and resorption of alveolar bone. Thus, special attention should be paid to the mechanism under lipopolysaccharide (<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>)\u2010induced periodontitis because <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content> is the major cause of periodontitis. However, to date, mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression in the <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced periodontitis has not been well characterized. In this study, we investigated mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression patterns in <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010treated <jats:styled-content style=\"fixed-case\">periodontal ligament cells (PDLC<\/jats:styled-content>s). Through mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> array and differential analysis, 22 up\u2010regulated mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and 28 down\u2010regulated mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010treated <jats:styled-content style=\"fixed-case\">PDLC<\/jats:styled-content>s were identified. Seven randomly selected up\u2010regulated (miR\u201021\u20105p, 498, 548a\u20105p) and down\u2010regulated (miR\u2010495\u20103p, 539\u20105p, 34c\u20103p and 7a\u20102\u20103p) mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were examined by <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, and the results proved the accuracy of the mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> array. Moreover, targets of these deregulated mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were analysed using the mi<jats:styled-content style=\"fixed-case\">RW<\/jats:styled-content>alk database. Database for Annotation, Visualization and Integration Discovery software were performed to analyse the Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes pathway of differential expression mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s, and the results shown that Toll\u2010like receptor signalling pathway, <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content> signalling pathway, transforming growth factor\u2010beta signalling pathway, mitogen\u2010activated protein kinase (<jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>) signalling pathway and other pathways were involved in the molecular mechanisms underlying <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced periodontitis. In conclusion, this study provides clues for enhancing our understanding of the mechanisms and roles of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s as key regulators of <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced periodontitis.<\/jats:p>","DOI":"10.1111\/jcmm.12819","type":"journal-article","created":{"date-parts":[[2016,3,18]],"date-time":"2016-03-18T11:48:56Z","timestamp":1458301736000},"page":"1329-1338","source":"Crossref","is-referenced-by-count":43,"title":["Micro<scp>RNA<\/scp> expression profile of human periodontal ligament cells under the influence of <i>Porphyromonas gingivalis <\/i><scp>LPS<\/scp>"],"prefix":"10.1111","volume":"20","author":[{"given":"Anqing","family":"Du","sequence":"first","affiliation":[{"name":"Department of Stomatology JinShan Hospital FuDan University JinShan District ShangHai 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