{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:46:20Z","timestamp":1761489980269},"reference-count":55,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,4,12]],"date-time":"2016-04-12T00:00:00Z","timestamp":1460419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81171539","81371764","81571951"],"award-info":[{"award-number":["81171539","81371764","81571951"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>New vaccines are needed to combat <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (<jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content>) infections. The currently employed Bacillus Calmette\u2010Gu\u00e9rin vaccine is becoming ineffective, due in part to the emergence of multidrug\u2010resistant tuberculosis (<jats:styled-content style=\"fixed-case\">MDR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>) strains and the reduced immune capacity in cases of <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content> coinfection. <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T cells play an important role in the protective immunity against <jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content> infections, and the identification of immunogenic <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T cell epitopes specific for <jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content> is essential for the design of peptide\u2010based vaccines. To identify <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T cell epitopes of <jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content> proteins, we screened a set of 94 <jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content> antigens for <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> class I A*11:01\u2010binding motifs. <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010A*11:01 is one of the most prevalent <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> molecules in Southeast Asians, and definition of T cell epitopes it can restrict would provide significant coverage for the Asian population. Peptides that bound with high affinity to purified <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> molecules were subsequently evaluated in functional assays to detect interferon\u2010\u03b3 release and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T cell proliferation in active pulmonary <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> patients. We identified six novel epitopes, each derived from a unique <jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content> antigen, which were recognized by <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T cells from active pulmonary <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> patients. In addition, a significant level of epitope\u2010specific T cells could be detected <jats:italic>ex vivo<\/jats:italic> in peripheral blood mononuclear cells from active <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> patients by an <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010A*11:01 dextramer carrying the peptide Rv3130c<jats:sub>194\u2010204<\/jats:sub> (from the <jats:styled-content style=\"fixed-case\">MTB<\/jats:styled-content> triacylglycerol synthase Tgs1), which was the most frequently recognized epitope in our peptide library. In conclusion, this study identified six dominant <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T cell epitopes that may be considered potential targets for subunit vaccines or diagnostic strategies against <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jcmm.12867","type":"journal-article","created":{"date-parts":[[2016,4,13]],"date-time":"2016-04-13T05:26:53Z","timestamp":1460525213000},"page":"1718-1728","source":"Crossref","is-referenced-by-count":6,"title":["Identification of <scp>HLA<\/scp>\u2010A*11:01\u2010restricted <i>Mycobacterium tuberculosis <\/i><scp>CD<\/scp>8<sup>+<\/sup> T cell epitopes"],"prefix":"10.1111","volume":"20","author":[{"given":"Su\u2010Dong","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Molecular Immunology School of Biotechnology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Nanfang Hospital Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shi\u2010Meng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Molecular Immunology School of Biotechnology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai\u2010Ping","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Severe Tuberculosis Medicine Guangzhou Chest Hospital Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Molecular Immunology School of Biotechnology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute of Molecular Immunology School of Biotechnology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Wen","sequence":"additional","affiliation":[{"name":"Institute of Molecular Immunology School of Biotechnology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian\u2010Chun","family":"He","sequence":"additional","affiliation":[{"name":"Institute of Molecular Immunology School of Biotechnology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Molecular Immunology School of Biotechnology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Molecular Immunology School of Biotechnology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.282.7.677"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/152.3.247"},{"key":"e_1_2_7_4_1","unstructured":"WHO Report\u2014Global tuberculosis control.Geneva:World Health Organization;2014. 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