{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:38:13Z","timestamp":1773301093617,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T00:00:00Z","timestamp":1466035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004319","name":"Shin Kong Wu Ho-Su Memorial Hospital","doi-asserted-by":"publisher","award":["SKH\u20108302\u2010102\u20100501"],"award-info":[{"award-number":["SKH\u20108302\u2010102\u20100501"]}],"id":[{"id":"10.13039\/501100004319","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2016,11]]},"abstract":"Abstract<\/jats:title>Epigallocatechin\u20103\u2010O\u2010gallate (EGCG<\/jats:styled-content>), derived from green tea, has been studied extensively because of its diverse physiological and pharmacological properties. This study evaluates the protective effect of EGCG<\/jats:styled-content> on angiotensin II<\/jats:styled-content> (Ang II<\/jats:styled-content>)\u2010induced endoglin expression in vitro<\/jats:italic> and in vivo<\/jats:italic>. Cardiac fibroblasts (CF<\/jats:styled-content>s) from the thoracic aorta of adult Wistar rats were cultured and induced with Ang II<\/jats:styled-content>. Western blotting, Northern blotting, real\u2010time PCR<\/jats:styled-content> and promoter activity assay were performed. Ang II<\/jats:styled-content> increased endoglin expression significantly as compared with control cells. The specific extracellular signal\u2010regulated kinase inhibitor SP<\/jats:styled-content>600125 (JNK<\/jats:styled-content> inhibitor), EGCG<\/jats:styled-content> (100 \u03bcM) and c\u2010Jun N\u2010terminal kinase (JNK<\/jats:styled-content>) siRNA<\/jats:styled-content> attenuated endoglin proteins following Ang II<\/jats:styled-content> induction. In addition, pre\u2010treated Ang II<\/jats:styled-content>\u2010induced endoglin with EGCG<\/jats:styled-content> diminished the binding activity of AP<\/jats:styled-content>\u20101 by electrophoretic mobility shift assay. Moreover, the luciferase assay results revealed that EGCG<\/jats:styled-content> suppressed the endoglin promoter activity in Ang II<\/jats:styled-content>\u2010induced CF<\/jats:styled-content>s by AP<\/jats:styled-content>\u20101 binding. Finally, EGCG<\/jats:styled-content> and the JNK<\/jats:styled-content> inhibitor (SP<\/jats:styled-content>600125) were found to have attenuated endoglin expression significantly in Ang II<\/jats:styled-content>\u2010induced CF<\/jats:styled-content>s, as determined through confocal microscopy. Following in vivo<\/jats:italic> acute myocardial infarction (AMI<\/jats:styled-content>)\u2010related myocardial fibrosis study, as well as immunohistochemical and confocal analyses, after treatment with endoglin siRNA<\/jats:styled-content> and EGCG<\/jats:styled-content> (50 mg\/kg), the area of myocardial fibrosis reduced by 53.4% and 64.5% and attenuated the left ventricular end\u2010diastolic and systolic dimensions, and friction shortening in hemodynamic monitor. In conclusion, epigallocatechin\u20103\u2010O\u2010gallate (EGCG<\/jats:styled-content>) attenuated the endoglin expression and myocardial fibrosis by anti\u2010inflammatory effect in vitro<\/jats:italic> and in vivo<\/jats:italic>, the novel suppressive effect was mediated through JNK<\/jats:styled-content>\/AP<\/jats:styled-content>\u20101 pathway.<\/jats:p>","DOI":"10.1111\/jcmm.12895","type":"journal-article","created":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T10:23:28Z","timestamp":1466072608000},"page":"2045-2055","source":"Crossref","is-referenced-by-count":22,"title":["Suppressive effect of epigallocatechin\u20103\u2010O\u2010gallate on endoglin molecular regulation in myocardial fibrosis in vitro<\/i> and in vivo<\/i>"],"prefix":"10.1111","volume":"20","author":[{"given":"Chiu\u2010Mei","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Emergency Medicine Shin Kong Wu Ho\u2010Su Memorial Hospital Taipei Taiwan"},{"name":"Faculty of Medicine School of Medicine Fu Jen Catholic University Taipei Taiwan"},{"name":"Institute of Injury Prevention and Control College of Public Health Taipei Medical University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hang","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine Shin Kong Wu Ho\u2010Su Memorial Hospital Taipei Taiwan"},{"name":"Institute of Injury Prevention and Control College of Public Health Taipei Medical University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bao\u2010Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Central Laboratory Shin Kong Wu Ho\u2010Su Memorial Hospital Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kou\u2010Gi","family":"Shyu","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular diseases Department of Internal Medicine Shin Kong Wu Ho\u2010Su Memorial Hospital Taipei Taiwan"},{"name":"Institute of Clinical Medical Sciences College of Medicine Taipei Medical University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,6,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(99)00630-0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2012.07.003"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.303697"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.31.6.1324"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38892-1"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000150369.68826.2f"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084188"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.111.080002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu7075230"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-013-0516-8"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0028-1088387"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00698.2006"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.58.6.1470"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2004.06.433"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf404479x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2006.11.026"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.223925"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.104.003434"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cjca.2014.12.026"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu5103779"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000343371"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2015.02.006"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2015.05.015"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.2119\/2004-00032.Aneja"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/hy1101.092971"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00380-011-0163-z"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehr065"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.114.000965"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000095181"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2010.01.007"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/10286020.2010.550883"}],"container-title":["Journal 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